Product Name	IL-10
Chinese Name	白细胞介素10抗体
Alias	Interleukin-10 precursor; Cytokine synthesis inhibitory factor; CSIF; Cytokine Synthesis Inhibitory Factor; IL 10; IL10A; Interleukin 10; Interleukin10; MGC126450; MGC126451; TGIF; IL10_HUMAN.  白介素10
literatures	Specific References  (60)     |     SL0698R has been referenced in 60 publications. [IF=24.897] Yuan, Xulei. et al. Systemic antibiotics increase microbiota pathogenicity and oral bone loss. INT J ORAL SCI. 2023 Jan;15(1):1-14  IHC ;  Mouse.   PubMed:36631439 [IF=20.011] Pylayeva-Gupta, Yuliya, et al. "IL-35 producing B cells promote the development of pancreatic neoplasia." Cancer discovery (2015): CD-15.  IF(IHC-P) ;  Mouse.   PubMed:26715643 [IF=13.273] Fangyu Qiao. et al. 4-Octyl itaconate modified demineralized bone matrix scaffold improves bone repair by regulating early inflammation. Chem Eng J. 2021 Dec;425:131490  WB,IF ;  Rat.   PubMed:10.1016/j.cej.2021.131490 [IF=11.092] Fangyu Qiao. et al. Hybrid Cell Membrane-Functionalized Matrixes for Modulating Inflammatory Microenvironment and Improving Bone Defect Repair. ADV HEALTHC MATER. 2023 Apr;:2203047  IF ;  Rat.   PubMed:37059691 [IF=10.273] Hou X et al. A 2D-2D heterojunction Bi2WO6/WS2-x as a broad-spectrum bactericide: Sulfur vacancies mediate the interface interactions between biology and nanomaterials. Biomaterials. 2020 Jun;243:119937.  IHC ;  S. aureus.   PubMed:32172032 [IF=10.041] Xiuhong Huang. et al. Chitooligosaccharide-europium (III) functional micron complex with visualized inflammation monitoring, immunomodulation and pro-vascularization activities for effective wound healing of pressure ulcers injury. Appl Mater Today. 2022 Mar;26:101310  IHC ;  Mouse.   PubMed:10.1016/j.apmt.2021.101310 [IF=8.786] Xiaoyu Guo. et al. Effects of Boric Acid Gel on Vaginal Candida albicans Infections and the Local Immune System in Mice. FRONT IMMUNOL. 2022; 13: 950215  IHC ;  Mouse.   PubMed:35958550 [IF=8.352] Ye Hea et al. Improved osteointegration by SEW2871-encapsulated multilayers on micro-structured titanium via macrophages recruitment and immunomodulation. Applied Materials Today 20 (2020) 100673  IF ;  Mouse.   PubMed:10.1016/j.apmt.2020.100673 [IF=7.525] Shen, Tianyi. et al. Exosomal AP000439.2 from clear cell renal cell carcinoma induces M2 macrophage polarization to promote tumor progression through activation of STAT3. CELL COMMUN SIGNAL. 2022 Dec;20(1):1-17  WB ;  Human.   PubMed:36153596 [IF=7.367] Yitian Du. et al. Engineered Microglia Potentiate the Action of Drugs against Glioma Through Extracellular Vesicles and Tunneling Nanotubes. 2021 Feb 28  IF,IHC ;  Mouse.   PubMed:33644993 [IF=7.328] Dongdong Yao. et al. Matrix stiffness regulates bone repair by modulating 12-lipoxygenase-mediated early inflammation. Mat Sci Eng C-Mater. 2021 Sep;128:112359  WB,IF,IHC ;  Rat.   PubMed:34474906 [IF=7.238] Chinthalapally V. Rao. et al. GSK3‐ARC/Arg3.1 and GSK3‐Wnt signaling axes trigger amyloid‐β accumulation and neuroinflammation in middle‐aged Shugoshin 1 mice. Aging Cell. 2020 Oct;19(10):e13221  IHC ;  Mouse.   PubMed:32857910 [IF=7.171] Li-Yu Chen. et al. Caspase-Mediated Cleavage of the Transcription Factor Sp3: Possible Relevance to Cancer and the Lytic Cycle of Kaposi’s Sarcoma-Associated Herpesvirus | Microbiology Spectrum. Microbiol Spectr. 2022 Feb;:  WB ;  Human.   PubMed:35019687 [IF=6.876] Lunardon Guilherme. et al. Set7 deletion attenuates isoproterenol-induced cardiac fibrosis and delays cardiac dysfunction. CLIN SCI. 2022 Nov;136(21):CS20220466  WB ;  Mouse.   PubMed:36285636 [IF=6.832] Yun Yu. et al. Geniposide promotes splenic Treg differentiation to alleviate colonic inflammation and intestinal barrier injury in ulcerative colitis mice. BIOENGINEERED. 2022;13(6):14616-14631  IHC ;  Mouse.   PubMed:36694912 [IF=6.832] Yanhong Li. et al. Therapeutic potential of human umbilical cord mesenchymal stem cells on aortic atherosclerotic plaque in a high-fat diet rabbit model. Stem Cell Res Ther. 2021 Dec;12(1):1-14  IHC ;  Rabbit.   PubMed:34266502 [IF=6.291] Si-Cheng Zhao. et al. Nickel sulfate exposure induces ovarian inflammation and fibrosis and decreases oocyte quality in mice. Ecotox Environ Safe. 2021 Nov;224:112634  IHC ;  Mouse.   PubMed:34392153 [IF=6.208] Jacqueline T. Hecht. et al. CurQ+, a Next-Generation Formulation of Curcumin, Ameliorates Growth Plate Chondrocyte Stress and Increases Limb Growth in a Mouse Model of Pseudoachondroplasia. INT J MOL SCI. 2023 Jan;24(4):3845  IHC ;  Mouse.   PubMed:36835255 [IF=5.568] Darius Häusler. et al. CNS inflammation after natalizumab therapy for multiple sclerosis: A retrospective histopathological and CSF cohort study. 2021 May 06  IHC ;  Human.   PubMed:33955606 [IF=5.561] Hui Li. et al. Blueberry–Mulberry Extract Alleviates Cognitive Impairment, Regulates Gut Metabolites, and Inhibits Inflammation in Aged Mice. FOODS. 2023 Jan;12(4):860  IHC ;  Mouse.   PubMed:36832936 [IF=5.396] Yan Zhang. et al. Polysaccharide from Ganoderma lucidum Alleviates Congnitive Impairment in a Mice Model of Chronic Cerebral Hypoperfusion by Regulating CD4+CD25+Foxp3+ regulatory T cells. Food Funct. 2022 Jan;:  WB ;  Mouse.   PubMed:35088782 [IF=5.296] Cao Y et al. Regulatory role of IKKɑ in myocardial ischemia/reperfusion injury by the determination of M1 versus M2 polarization of macrophages. J Mol Cell Cardiol. 2018 Oct;123:1-12.   IHC-P ;  Mouse.   PubMed:30153439 [IF=5.279] Yan Zhang. et al. Ganoderic Acid A To Alleviate Neuroinflammation of Alzheimer’s Disease in Mice by Regulating the Imbalance of the Th17/Tregs Axis. J Agr Food Chem. 2021;69(47):14204–14214  WB ;  Mouse.   PubMed:34798773 [IF=5.19] Han, Sheng, et al. "LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4." Journal of Experimental & Clinical Cancer Research 36.1 (2017): 83.  WB ;  Human.   PubMed:28641579 [IF=5.039] Wang Y et al. Aspirin inhibits inflammation and scar formation in the injury tendon healing through regulating JNK/STAT‐3 signalling pathway. Cell Prolif. 2019 Jun 21:e12650.  WB ;  Rat.   PubMed:31225686 [IF=4.996] Ren, Xiaoli. et al. Expression and significance of IL-6 and IL-8 in canine mammary gland tumors. SCI REP-UK. 2023 Jan;13(1):1-9  IHC,WB ;  Dog.   PubMed:36693957 [IF=4.996] Tripson Mark. et al. Cannabidiol inhibits neuroinflammatory responses and circuit-associated synaptic loss following damage to a songbird vocal pre-motor cortical-like region. SCI REP-UK. 2023 May;13(1):1-18  IF,WB ;  Zebra finches.   PubMed:37193782 [IF=4.932] Xueli Bai. et al. Regulatory role of methionine enkephalin in myeloid-derived suppressor cells and macrophages in human cutaneous squamous cell carcinoma. Int Immunopharmacol. 2021 Oct;99:107996  IHC ;  Mouse.   PubMed:34311187 [IF=4.831] Lushuang Xie. et al. Inflammatory factors and amyloid β-induced microglial polarization promote inflammatory crosstalk with astrocytes.. Aging-Us. 2020 Nov 30; 12(22): 22538–22549  IHC ;  Rat.   PubMed:33196457 [IF=4.486] Shanshan Zhao. et al. IL‐27 Rα+ cells promoted allorejection via enhancing STAT1/3/5 phosphorylation. J Cell Mol Med. 2020 Sep;24(18):10756-10767  IF,IHC ;  Mouse.   PubMed:32761753
Research Area	Cell biology  immunology  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Chicken, Pig, Rabbit, Sheep, Guinea Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	18kDa
Detection molecular weight	21 kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human IL-10: 101-178/178
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. [provided by RefSeq, May 2020] Function: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Subunit: Homodimer. Subcellular Location: Secreted. Tissue Specificity: Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. Similarity: Belongs to the IL-10 family. SWISS: P22301 Gene ID: 3586 Database links: Entrez Gene: 3586 Human Entrez Gene: 16153 Mouse Entrez Gene: 25325 Rat Omim: 124092 Human SwissProt: P22301 Human SwissProt: P18893 Mouse SwissProt: P29456 Rat Unigene: 193717 Human Unigene: 874 Mouse Unigene: 9868 Rat 白细胞介素-10(IL-10)是主要由Th2细胞所产生的一种cell factor，活化的B细胞、单核巨噬细胞和枯否氏细胞（Kupffer's cell）等也可产生IL-10。它是以抑制Th细胞克隆cell factor合成为特点的多效免疫调节因子。 白介素-10是维护cell factor网络平衡的重要负调节因子。其作用机制可能是降低抗原递呈细胞MHCⅡ类抗原表达，或诱导抗原递呈细胞产生另一种cell factor，改变细胞内信号传递途径，从而选择性抑制某些cell factormRNA转录，并与Th2细胞产生的IL-4、IL-5有协同作用。
Product Picture	Sample: Lane 1: THP-1 (Human) Cell Lysate at 30 ug Lane 2: THP-1-LPS (Human) Cell Lysate at 30 ug Primary: Anti-IL-10 (SL0698R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18.6 kD Observed band size: 17 kD Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-IL-10 (SL0698R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 18 kD Paraformaldehyde-fixed, paraffin embedded (Human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-10) Polyclonal Antibody, Unconjugated (SL0698R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IL-10 Polyclonal Antibody, Unconjugated(SL0698R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IL-10 Polyclonal Antibody, Unconjugated(SL0698R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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