Product Name	CD147
Chinese Name	Extracellular matrix金属蛋白酶诱导因子抗体
Alias	TCSF; Emmprin; 5A11 antigen; 5F7; Basigin (Ok blood group); Basigin; Blood brain barrier HT7 antigen; BSG; CD 147; CD147 antigen; Collagenase stimulatory factor; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M6; M6 leukocyte activation antigen; neurothelin; OK; OK blood group; OK blood group antigen; TCSF; Tumor cell derived collagenase stimulatory factor; BASI_HUMAN.
literatures	Specific References  (4)     |     SL0684R has been referenced in 4 publications. [IF=4.872] Muhammad Fakhar-e-Alam Kulyar. et al. Cluster of differentiation 147 (CD147) expression is linked with thiram induced chondrocyte’s apoptosis via Bcl-2/Bax/Caspase-3 signalling in tibial growth plate under chlorogenic acid repercussion. Ecotox Environ Safe. 2021 Apr;213:112059  WB,IF ;  Chicken.   PubMed:33647747 [IF=3.174] Xiaole Tong et al. LPS-induced inflammation disorders bone modeling and remodeling by inhibiting angiogenesis and disordering osteogenesis in chickens. Inflamm Res. 2020 Aug;69(8):765-777.  WB ;  Chicken.   PubMed:32444883 [IF=3.12] Tsai, Wen-Chiuan, et al. "EMMPRIN expression positively correlates with WHO grades of astrocytomas and meningiomas." Journal of Neuro-Oncology (2013): 1-10.  Human.   PubMed:23817808 [IF=2.19] Cui, K., et al. "Transplantation of Autologous Bone Marrow Mononuclear Cells Regulates Inflammation in a Rabbit Model of Carotid Artery Atherosclerosis." Journal of Vascular Research 53.3-4 (2016): 196-205.  WB ;  Rabbit.   PubMed:27788500
Research Area	Tumour  immunology  Synthesis and Degradation  Cell Surface Molecule
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Rabbit, Guinea Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	40kDa
Detection molecular weight	55-65 kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human TCSF: 301-385/385 <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Function: Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. Subunit: Forms homooligomers in a cis-dependent manner on the plasma membrane. Forms heterooligomers of isoform 2 and isoform 3. Forms a complex with MMP1 at the tumor cell surface. Interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. Interacts with ATP1B2, MAG and L1CAM. Interacts with AJAP1. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Melanosome. Tissue Specificity: Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas. Post-translational modifications: N-glycosylated. Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: P35613 Gene ID: 682 Database links: Entrez Gene: 682 Human Entrez Gene: 12215 Mouse Entrez Gene: 25246 Rat Omim: 109480 Human SwissProt: P35613 Human SwissProt: P18572 Mouse SwissProt: P26453 Rat Unigene: 501293 Human Synthesis and Degradation（Synthesis and Degradation） 主要由Tumour细胞合成，刺激纤维母细胞产生MMP
Product Picture	Sample: Liver(Mouse) Lysate at 40 ug Placenta(Mouse) Lysate at 40 ug HepG2 Cell Lysate at 40 ug Primary: Anti-CD147 (SL0684R) at 1/300 dilution  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution  Predicted band size: 40 kD Observed band size: 60 kD Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-CD147 (Bs-0684R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 50 kD Sample: Cerebellum (Mouse) Lysate at 40 ug Primary: Anti-CD147 (Bs-0684R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 50 kD Sample: Lane 1: Liver (Mouse) Lysate at 40 ug Lane 2: Liver (Rat) Lysate at 40 ug Lane 3: HepG2 (Human) Cell Lysate at 30 ug Lane 4: U251 (Human) Cell Lysate at 30 ug Lane 5: MCF-7 (Human) Cell Lysate at 30 ug Primary: Anti-CD147 (SL0684R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50-60 kD Observed band size: 60 kD Paraformaldehyde-fixed, paraffin embedded (Mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD147) Polyclonal Antibody, Unconjugated (SL0684R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD147) Polyclonal Antibody, Unconjugated (SL0684R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD147/TCSF/Emmprin Polyclonal Antibody, Unconjugated(SL0684R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control (blue line): MCF7 (blue). Primary Antibody (green line):Rabbit Anti-CD147 antibody(SL0684R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 2% paraformaldehyde for 10 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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