Product Name	MRP1/ABCC1
Chinese Name	多药耐药相关蛋白1抗体
Alias	Multidrug Resistanec-Associated Protein 1; ABC 29; ABC29; ABCC 1; ABCC; ABCC1; ATP binding cassette sub family C (CFTR/MRP) member 1; ATP binding cassette sub-family C member 1; DKFZp686N04233; DKFZp781G125; GS X; GSX; Leukotriene C(4) transporter; LTC4 transporter; MRP 1; MRP; Multidrug resistance associated protein 1; Multidrug resistance protein; Multiple drug resistance associated protein; Multiple drug resistance protein 1; MRP1_HUMAN.
literatures	Specific References  (8)     |     SL0657R has been referenced in 8 publications. [IF=6.93] Peng, Lei, et al. "Development of a novel orthotopic non-small cell lung cancer model and therapeutic benefit of 2’-(2-bromohexadecanoyl)-docetaxel conjugate nanoparticles." Nanomedicine: Nanotechnology, Biology and Medicine (2014).  IHC-P ;  Mouse.   PubMed:24709328 [IF=5.988] Zipeng Gong. et al. Pharmacokinetic Differences of Wuji Pill Components in Normal and Chronic Visceral Hypersensitivity Irritable Bowel Syndrome Rats Attributable to Changes in Tight Junction and Transporters. FRONT PHARMACOL. 2022; 13: 948678  IHC, IF ;  Rat.   PubMed:35873589 [IF=4.192] Peng Yang. et al. Cucurbitacin E Chemosensitizes Colorectal Cancer Cells via Mitigating TFAP4/Wnt/β-Catenin Signaling. J Agr Food Chem. 2020;68(48):14148–14160  WB ;  Human.   PubMed:33205649 [IF=3.33] Chen et al. Timosaponin A-III reverses multi-drug resistance in human chronic myelogenous leukemia K562/ADM cells via downregulation of MDR1 and MRP1 expression by inhibiting PI3K/Akt signaling pathway. (2016) Int.J.Oncol. 48:2063-70  FC/FACS,WB ;  Human.   PubMed:26984633 [IF=3.07] Wen-Qing, Hu, Peng Chun-Wei, and Li Yan. "The expression and significance of P-glycoprotein, lung resistance protein and multidrug resistance-associated protein in gastric cancer." Journal of Experimental & Clinical Cancer Research 28 (2009).  Human.   PubMed:19930704 [IF=2.248] Wei Shunying. et al. Overexpression of circ_CELSR1 facilitates paclitaxel resistance of ovarian cancer by regulating miR-149-5p/SIK2 axis. Anti-Cancer Drug. 2021 Jun;32(5):496-507  WB ;  Human.   PubMed:33735118 [IF=1.91] Yan, Yong‐Rong, et al. "Epithelial‐to‐mesenchymal transition is involved in BCNU resistance in human glioma cells." Neuropathology (2014).  WB ;  Human.   PubMed:19930704 [IF=1.664] Yi et al. Solanine reverses multidrug resistance in human myelogenous leukemia K562/ADM cells by downregulating MRP1 expression. (2018) Oncol.Lett. 15:10070-10076  WB ;   PubMed:29928376
Research Area	Tumour  Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Chicken, Cow, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	170kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human MRP1: 501-600/1531 <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a multispecific organic anion transporter, with oxidized glutatione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Alternatively spliced variants of this gene have been described but their full-length nature is unknown. [provided by RefSeq, Apr 2012] Function: Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Lung, testis and peripheral blood mononuclear cells. Similarity: Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains. SWISS: O35379 Gene ID: 17250 Database links: Entrez Gene: 4363 Human Entrez Gene: 17250 Mouse Entrez Gene: 24565 Rat Omim: 158343 Human SwissProt: P33527 Human SwissProt: O35379 Mouse SwissProt: Q8CG09 Rat Unigene: 391464 Human Unigene: 196634 Mouse Unigene: 10495 Rat 多药耐药相关蛋白1(MRP1)是MRP家族中的一个成员之一，基因的染色体位点是16p13.1，它编码分子量是170-190kDa的MRP1蛋白。 MRP1又称ABCC1主要表达在消化系统中的肝脏、结肠和胰腺组织中，常用于多种Tumour的多药耐药机制的研究。
Product Picture	Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MRP1 Polyclonal Antibody, Unconjugated(SL0657R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MRP1 Polyclonal Antibody, Unconjugated(SL0657R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MRP1 Polyclonal Antibody, Unconjugated(SL0657R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: K562. Primary Antibody (green line): Rabbit Anti-MRP1/ABCC1 antibody (SL0657R) Dilution: 2ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control: A549. Primary Antibody (green line): Rabbit Anti-ABCC1 antibody (SL0657R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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