Product Name	Growth hormone receptor
Chinese Name	生长激素受体抗体
Alias	GH receptor; GHBP; GHR; Growth hormone receptor precursor; Serum binding protein; Somatotropin receptor; GHR_HUMAN.
literatures	Specific References  (12)     |     SL0654R has been referenced in 12 publications. [IF=5.738] Ahmed O. Kaseb. et al. Blockade of growth hormone receptor signaling by using pegvisomant: A functional therapeutic strategy in hepatocellular carcinoma.. FRONT ONCOL. 2022 Oct;12:986305-986305  IHC, IF ;  Mouse, Human.   PubMed:36276070 [IF=4.24] Hetz, Jennifer A., et al. "Growth axis maturation is linked to nutrition, growth and developmental rate." Molecular and Cellular Endocrinology (2015).  WB ;   PubMed:25896544 [IF=4.12] Cui et al. Generation of a miniature pig disease model for human Laron syndrome. (2015) Sci.Rep. 5:15603  WB ;  Porcine.   PubMed:26511035 [IF=3.86] Chu, Meiqiang, et al. "MicroRNA-126 participates in lipid metabolism in mammary epithelial cells." Molecular and Cellular Endocrinology (2017).  WB ;  Human.   PubMed:28599789 [IF=3.471] Fengyang Wu. et al. Effects of zearalenone on ovarian development of prepubertal gilts through growth hormone axis. FRONT VET SCI. 2022; 9: 950063  IHC ;  Pig.   PubMed:35990263 [IF=2.51] Yang, Hai Li, et al. "Effect of suppressor of cytokine signaling 2 (SOCS2) on fat metabolism induced by growth hormone (GH) in porcine primary adipocyte." Molecular biology reports 39.9 (2012): 9113-9122.  WB ;  Pig.   PubMed:22729878 [IF=2.445] Xie W et al. Seasonal expressions of growth hormone receptor, insulin-like growth factor 1 and insulin-likegrowth factor 1 receptor in the scented glands of the muskrats (Ondatra zibethicus). Gen Comp Endocrinol. 2019 May 20;281:58-66.  IHC-P&WB ;  muskrat.   PubMed:31121166 [IF=2.23] Chen et al. Effect of sericin on diabetic hippocampal growth hormone/insulin-like growth factor 1 axis. (2013) Neural.Regen.Res. 8:1756-64  WB ;  Rat.   PubMed:25206472 [IF=2.02] Wang, Zheng, et al. "RhGH attenuates ischemia injury of intrahepatic bile ducts relating to liver transplantation." Journal of Surgical Research 171.1 (2011): 300-310.  IHC-P ;  Rat.   PubMed:20462597 [IF=1.84] Zhao, Yong, et al. "Inhibition of peripubertal sheep mammary gland development by cysteamine through reducing progesterone and growth factor production." Theriogenology (2016).  WB ;  Sheep.   PubMed:28043364 [IF=1.27] Song, Cheng-Jun, et al. "Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes." International Journal of Clinical and Experimental Medicine 8.7 (2015): 10411-10419.  WB ;  Rat.   PubMed:26379831 [IF=0.86] Zhou et al. Effects of zearalenone on the localization and expression of the growth hormone receptor gene in the uteri of post-weaning piglets. (2018) Asian-Australas.J.Anim.Sci. 31:32-39  IHC-P ;  Pig.   PubMed:28920404
Research Area	Cell biology  Developmental biology  Signal transduction  Growth factors and hormones  Cell differentiation
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	68kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human GHR: 101-200/451 <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]. Function: Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling. Isoform 2 up-regulates the production of GHBP and acts as a negative inhibitor of GH signaling. Subunit: On growth hormone (GH) binding, forms homodimers and binds JAK2 via a box 1-containing domain. Binding to SOCS3 inhibits JAK2 activation, binding to CIS and SOCS2 inhibits STAT5 activation. Interacts with ADAM17. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Note=On growth hormone binding, GHR is ubiquitinated, internalized, down-regulated and transported into a degradative or non-degradative pathway. Isoform 2: Cell membrane; Single-pass type I membrane protein. Note=Remains fixed to the cell membrane and is not internalized. Growth hormone-binding protein: Secreted. Note=Complexed to a substantial fraction of circulating GH. Tissue Specificity: Expressed in various tissues with high expression in liver and skeletal muscle. Isoform 4 is predominantly expressed in kidney, bladder, adrenal gland and brain stem. Isoform 1 expression in placenta is predominant in chorion and decidua. Isoform 4 is highly expressed in placental villi. Isoform 2 is expressed in lung, stomach and muscle. Low levels in liver. Post-translational modifications: The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. Shedding is inhibited by growth hormone (GH) binding to the receptor probably due to a conformational change in GHR rendering the receptor inaccessible to ADAM17. On GH binding, phosphorylated on tyrosine residues in the cytoplasmic domain by JAK2. On ligand binding, ubiquitinated on lysine residues in the cytoplasmic domain. This ubiquitination is not sufficient for GHR internalization. DISEASE: Laron syndrome (LARS) [MIM:262500]: A severe form of growth hormone insensitivity characterized by growth impairment, short stature, dysfunctional growth hormone receptor, and failure to generate insulin-like growth factor I in response to growth hormone. Note=The disease is caused by mutations affecting the gene represented in this entry. Idiopathic short stature autosomal (ISSA) [MIM:604271]: Short stature is defined by a subnormal rate of growth. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the type I cytokine receptor family. Type 1 subfamily. Contains 1 fibronectin type-III domain. SWISS: P10912 Gene ID: 2690 Database links: Entrez Gene: 2690 Human Entrez Gene: 14600 Mouse Entrez Gene: 25235 Rat Omim: 600946 Human SwissProt: P10912 Human SwissProt: P16882 Mouse SwissProt: P16310 Rat Unigene: 125180 Human Unigene: 684631 Human Unigene: 3986 Mouse Unigene: 2178 Rat 生长激素受体(growth hormone receptor，GHR)是cell factor/造血因子受体超级家族成员之一。 生长激素受体通过二聚体的形式和生长激素(growth hormone, GH)相结合，然后诱发Janus 激酶2 (Janus kinase 2，JAK2：是很多cell factor、生长因子及Interferon的重要信号传感器)等cell factor酪氨酸磷酸化并通过4条不同的途径将信号传入细胞内从而产生一系列的生理效应。
Product Picture	Sample: Lane 1: Testis (Mouse) Lysate at 40 ug Lane 2: Cerebrum (Mouse) Lysate at 40 ug Lane 3: Testis (Rat) Lysate at 40 ug Lane 4: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-Growth hormone receptor (SL0654R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130/110 kD Observed band size: 130 kD Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GHR Polyclonal Antibody, Unconjugated(SL0654R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining This image was generously provided by Brandon Menzies, PhD, from The University of Melbourne. Paraffin embedded tammar wallaby (Macropus eugenii) liver labeled with Rabbit Anti-GHR Polyclonal Antibody, Unconjugated (SL0654R) at 1:300 followed by conjugation to a secondary antibody and staining. Positive control: A549 cells Concebtration: 1μg/10^6 cells Incubation conditions: Avoid light , 30 minutes on the ice. Blank control: A549(blue), the cells were fixed with 2% paraformaldehyde (10 min) . Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 5μl in 100 μL1X PBS containing 0.5% BSA(green).

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