Product Name	CD68
Chinese Name	CD68抗体
Alias	CD 68; CD68 antigen; CD68 molecule; DKFZp686M18236; GP110; Macrophage antigen CD68 (microsialin); macrosialin; SCARD1; Scavenger receptor class D member 1; macrosialin isoform A precursor; CD68_MOUSE; Macrosialin; Gp110; scavenger receptor class D, member 1; macrophage antigen CD68; LAMP4.
literatures	Specific References  (61)     |     SL0649R has been referenced in 61 publications. [IF=18.027] Yue Zhou. et al. Turning Hot into Cold: Immune Microenvironment Reshaping for Atherosclerosis Attenuation Based on pH-Responsive shSiglec-1 Delivery System. ACS NANO. 2022;XXXX(XXX):XXX-XXX  IF ;  Mouse.   PubMed:35762565 [IF=15.881] Tang Gao. et al. Biomimetic Glucan Particles with Aggregation-Induced Emission Characteristics for Noninvasive Monitoring of Transplant Immune Response. Acs Nano. 2021;XXXX(XXX):XXX-XXX  IF,IHC ;  Mouse.   PubMed:34264052 [IF=14.808] Xue Gong. et al. Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation. J Clin Invest. 2021 Dec 15;131(24):e147031  IF ;  Mouse.   PubMed:34907911 [IF=10.652] Zhuo Liang. et al. Exosome derived from mesenchymal stem cells mediates hypoxia-specific BMP2 gene delivery and enhances bone regeneration. Chem Eng J. 2021 Oct;422:130084  IF ;  Rabbit.   PubMed:10.1016/j.cej.2021.130084 [IF=8.947] Kailei Ding. et al. A PEGylation method of fabricating bioprosthetic heart valves based on glutaraldehyde and 2-amino-4-pentenoic acid co-crosslinking with improved antithrombogenicity and cytocompatibility. Acta Biomater. 2022 Mar;:  IHC ;  Rat.   PubMed:35365404 [IF=8.784] Wang et al. Programmed cell death-4 deficiency prevents diet-induced obesity, adipose tissue inflammation, and insulin resistance. (2013) Diabete. 62:4132-43  IHC-P ;  Mouse.   PubMed:23990362 [IF=8.322] Zhou, Hang. et al. AXL kinase-mediated astrocytic phagocytosis modulates outcomes of traumatic brain injury. J Neuroinflamm. 2021 Dec;18(1):1-17  WB ;  Mouse.   PubMed:34233703 [IF=7.78] Zhou,et al.Ultrasensitive label-free optical microfiber coupler biosensor for detection of cardiac troponin I based on interference turning point effect.(2018) Biosensors & Bioelectronics. 106:99-104.  Other ;   PubMed:29414096 [IF=7.419] Chihiro Kishi. et al. Inhaled volatile β-caryophyllene is incorporated into the aortic wall and attenuates nicotine-induced aorta degeneration via a CB2 receptor-dependent pathway. BIOMED PHARMACOTHER. 2022 Sep;153:113423  IHC ;  Mouse.   PubMed:10.1016/j.biopha.2022.113423 [IF=7.126] Vendrov AE et al.NOXA1-dependent NADPH oxidase regulates redox signaling and phenotype of vascular smooth muscle cell during atherogenesis.Redox Biol. (2018) Redox Biol. 21:101063.  WB ;  Mouse.   PubMed:30576919 [IF=7.038] Ivana Dokic. et al. Neuroprotective Effects of Ultra-High Dose Rate FLASH Bragg Peak Proton Irradiation. Int J Radiat Oncol. 2022 Feb;:  IF ;  Mouse.   PubMed:10.1016/j.ijrobp.2022.02.020 [IF=6.529] Hong Zhen. et al. VitA or VitD ameliorates bronchopulmonary dysplasia by regulating the balance between M1 and M2 macrophages. Biomed Pharmacother. 2021 Sep;141:111836  IHC ;  Rat.   PubMed:34214728 [IF=6.383] Guo G et al. Radical polymerization-crosslinking method for improving extracellular matrix stability in bioprosthetic heart valves with reduced potential for calcification and inflammatory response.(2018) Acta Biomater. Oct 14. pii: S1742-7061(18)30616-0.  IHC ;  Rat.   PubMed:30326277 [IF=6.29] Müller, Irene, et al. "Pathogenic Role of the Damage-Associated Molecular Patterns S100A8 and S100A9 in Coxsackievirus B3–Induced Myocarditis." Circulation: Heart Failure 10.11 (2017): e004125.  IHC-F ;  Mouse.   PubMed:29158436 [IF=6.208] Zheng Kou. et al. Mesenchymal Stem Cells Pretreated with Collagen Promote Skin Wound-Healing. INT J MOL SCI. 2023 Jan;24(10):8688  IF,IHC ;  Mouse.   PubMed:37240027 [IF=5.96] Savvatis, Konstantinos, et al. "Interleukin-6 receptor inhibition modulates the immune reaction and restores titin phosphorylation in experimental myocarditis." Basic Research in Cardiology 109.6 (2014): 1-14.  IHC-P ;  Mouse.   PubMed:25344085 [IF=5.714] Yani He. et al. Glycolytic reprogramming controls periodontitis-associated macrophage pyroptosis via AMPK/SIRT1/NF-κB signaling pathway. INT IMMUNOPHARMACOL. 2023 Jun;119:110192  IHC,IF ;  Human.   PubMed:37068341 [IF=5.546] Ye Qi. et al. A simple, quick, and cost-effective strategy to fabricate polycaprolactone/silk fibroin nanofiber yarns for biotextile-based tissue scaffold application. EUR POLYM J. 2023 Jan;:111863  IF ;  Mouse.   PubMed:10.1016/j.eurpolymj.2023.111863 [IF=5.31] Yibei Wang. et al. NOD2 deficiency confers a pro-tumorigenic macrophage phenotype to promote lung adenocarcinoma progression. 2021 Jul 16  IHC ;  Human.   PubMed:34268854 [IF=5.307] Juan Zhang. et al. The antimicrobial peptide LK2(6)A(L) exhibits anti-inflammatory activity by binding to the myeloid differentiation 2 domain and protects against LPS-induced acute lung injury in mice. BIOORG CHEM. 2023 Mar;132:106376  IHC ;  Mouse.   PubMed:36706531 [IF=5.23] Ganguly, Rituparna, et al. "Oral chromium picolinate impedes hyperglycemia-induced atherosclerosis and inhibits proatherogenic protein TSP-1 expression in STZ-induced type 1 diabetic ApoE−/− mice." Scientific Reports 7 (2017).  IHC-P ;  Mouse.   PubMed:28345659 [IF=5.116] Yu Satoet al. Prophylactic therapy with Human amniotic fluid stem cells improved survival in a rat model of lipopolysaccharide-induced neonatal sepsis through immunomodulation via aggregates with peritoneal macrophages. Stem Cell Res Ther . 2020 Jul 20;11(1):300.  other ;   PubMed:32690106 [IF=5.11] Vendrov, Aleksandr E., et al. "Attenuated Superoxide Dismutase 2 Activity Induces Atherosclerotic Plaque Instability During Aging in Hyperlipidemic Mice." Journal of the American Heart Association 6.11 (2017): e006775.  IHC-F ;  Mouse.   PubMed:29079564 [IF=5.047] Guo G et al. Hydrogel hybrid porcine pericardium for the fabrication of pre-mounted TAVI valve with improved biocompatibility. Journal of Materials Chemistry B. 2019.  IHC-P ;  Rat.   PubMed:doi:10.1039/c8tb02565g [IF=4.94] Xu, Xiaoyang, et al. "Simvastatin promotes NPC1‐mediated free cholesterol efflux from lysosomes through CYP7A1/LXRα signalling pathway in oxLDL‐loaded macrophages." Journal of Cellular and Molecular Medicine (2016).  IF(ICC) ;  Mouse.   PubMed:27629819 [IF=4.498] Wu et al. Chronic inflammation exacerbates glucose metabolism disorders in C57BL/6J mice fed with high-fat diet. (2013) J.Endocrino. 219:195-204  IHC-P ;  Mouse.   PubMed:24029730 [IF=4.396] Fan Yang. et al. Visible light–induced cross-linking of porcine pericardium for the improvement of endothelialization, anti-tearing, and anticalcification properties. 2021 Jul 09  IHC ;  Rat.   PubMed:34245103 [IF=4.35] Hong, Hye Kyoung, et al. "Neonatal systemic inflammation in rats alters retinal vessel development and simulates pathologic features of retinopathy of prematurity." Journal of Neuroinflammation 11.1 (2014): 87.  IHC-F ;  Rat.   PubMed:24886524 [IF=4.323] Nishikawa,et al.Highly Dispersible and Bioavailable Curcumin but not Native Curcumin Induces Brown-Like Adipocyte Formation in Mice.(2018) Molecular Nutrition & Food Research. 62:.  IF(IHC-P) ;  Mouse.   PubMed:29334590 [IF=4.26] Nishizawa et al. IGF-I induces senescence of hepatic stellate cells and limits fibrosis in a p53-dependent manner. (2016) Sci.Re. 6:34605  IHC-P ;  Mouse.   PubMed:27721459
Research Area	immunology  Cell Surface Molecule
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IF=1:200-800 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	37kDa
Detection molecular weight	110-140KDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from mouse CD68: 21-120/335 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008] Function: Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. Subcellular Location: Isoform Short: Cell membrane; Single-pass type I membrane protein. Isoform Long: Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Tissue Specificity: Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. Similarity: Belongs to the LAMP family. SWISS: P31996 Gene ID: 12514 Database links: Entrez Gene: 968 Human Entrez Gene: 12514 Mouse Omim: 153634 Human SwissProt: P34810 Human SwissProt: P31996 Mouse Unigene: 647419 Human Unigene: 15819 Mouse CD68为巨噬细胞抗原又称酸性溶霉菌glycoprotein，表达在单核、巨嗜、粒细胞和一些梭形细胞呈阳性表达。C该抗体对于鉴定组织切片中的巨噬细胞具有重要作用。它能够鉴定多种组织中的巨噬细胞，包括脾脏红髓、肠道固有层、肺泡、骨髓中的枯否细胞和巨噬细胞。也能够识别骨髓前体细胞，外周血粒细胞以及浆细胞样T细胞。
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Human ovarian cancer); Antigen retrieval by microwaving in sodium citrate buffer (pH6.0) for 5min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (SL0649R) at 1:500 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by microwaving in sodium citrate buffer (pH6.0) for 5min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (SL0649R) at 1:500 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by microwaving in sodium citrate buffer (pH6.0) for 5min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (SL0649R) at 1:500 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (SL0649R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (SL0649R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (SL0649R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by microwaving in sodium citrate buffer (pH6.0) for 5min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (SL0649R) at 1:500 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human lung); Antigen retrieval by microwaving in sodium citrate buffer (pH6.0) for 5min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (SL0649R) at 1:500 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (SL0649R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (SL0649R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (SL0649R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (SL0649R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat myocardium tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD68 Polyclonal Antibody, Unconjugated(SL0649R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (SL0649R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-FITC) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by microwaving in sodium citrate buffer (pH6.0) for 5min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (SL0649R) at 1:500 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-AF488) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (SL0649R) at 1:500 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-AF488) for 90 minutes, and DAPI for nuclei staining.

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