Product Name	Integrin alpha 4
Chinese Name	整合素α4（CD49d）抗体
Alias	Alpha 4 subunit of VLA 4 receptor;ITG-α4: Antigen CD49D; CD 49d ; CD49d; CD49d antigen; CDw49d; IA4; Integrin alpha IV; ITGA 4; ITGA4; LPAM23; MGC90518; Very Late Activation Antigen 4; Very Late Activation Protein 4 Receptor Alpha 4 Subunit; VLA 4; VLA4; integrin alpha-4; ITA4_HUMAN; Integrin α4; Integrin α 4.
literatures	Specific References  (1)     |     SL0641R has been referenced in 1 publications. [IF=3.53] Lee, Tao-Chen, et al. "Comparison of Surface Markers between Human and Rabbit Mesenchymal Stem Cells." PLOS ONE 9.11 (2014): e111390.  Rabbit.   PubMed:25380245
Research Area	Cell biology  immunology  Signal transduction  Stem cells  Cell adhesion molecule
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1μg/Test  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	111kDa
Detection molecular weight	150 kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Integrin alpha 4: 551-650/1032 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015] Function: Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. Subunit: Heterodimer of an alpha and a beta subunit. Subcellular Location: Membrane; Single-pass type I membrane protein. Post-translational modifications: Phosphorylation on Ser-1027 inhibits PXN binding. Similarity: Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats. SWISS: P13612 Gene ID: 3676 Database links: Entrez Gene: 3676 Human Entrez Gene: 16401 Mouse Omim: 192975 Human SwissProt: P13612 Human SwissProt: Q00651 Mouse Unigene: 440955 Human Unigene: 31903 Mouse 整合素-α-4型 Integrin α4 (CD49d)整合素-α是介导细胞与Extracellular matrix粘附作用的主要因子。
Product Picture	Sample: Lane 1: Human A549 cell lysates Lane 2: Human MCF-7 cell lysates Primary: Anti-Integrin alpha 4 (SL0641R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 111 kDa Observed band size: 150 kDa Sample: Lane 1: Normal human Jurkat cell lysates Lane 2: Jurkat treated with PMA 125ng/ml 30min Primary: Anti-Integrin alpha 4 (SL0641R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 111 kDa Observed band size: 150 kDa Blank control: U937(blue). Primary Antibody: Rabbit Anti-Integrin alpha 4 antibody(SL0641R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (SL0641R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed. Blank control:THP-1. Primary Antibody (green line): Rabbit Anti-Integrin alpha 4 antibody (SL0641R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Black line : Positive blank control (U937); Negative blank control (MCF7） Green line : Primary Antibody (Rabbit Anti-Integrin alpha 4 antibody (SL0641R) ) Orange line：Isotype Control Antibody (Rabbit IgG) . Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF647) U937（Positive）and MCF7（Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Integrin alpha 4 Antibody(SL0641R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

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Totalgoods,subtotals:￥Checkout