Product Name	Aquaporin 4
Chinese Name	水Channel protein4抗体
Alias	aquaporin 4; WCH4; Mercurial-insensitive water channel; MIWC; AQP 4; AQP4; Aquaporin4; HMIWC 2; HMIWC2; Mercurial insensitive water channel; MGC22454; MIWC; AQP4_HUMAN; Aquaporin-4; AQP-4.
literatures	Specific References  (9)     |     SL0634R has been referenced in 9 publications. [IF=7.675] Yimeng Fan. et al. Pingwei San Ameliorates Spleen Deficiency-Induced Diarrhea through Intestinal Barrier Protection and Gut Microbiota Modulation. ANTIOXIDANTS-BASEL. 2023 May;12(5):1122  IHC ;  Rat.   PubMed:37237988 [IF=6.42] Cao et al. Hypertonic saline reduces lipopolysaccharide-induced mouse brain edema through inhibiting aquaporin 4 expression. (2012) Crit.Care. 16:R186  WB/FC ;  Mouse.   PubMed:23036239 [IF=3.887] Huijun LI. et al. Screening the effective components in treating dampness stagnancy due to spleen deficiency syndrome and elucidating the potential mechanism of Poria water extract. CHIN J NAT MEDICINES. 2023 Feb;21:83  WB ;  Rat.   PubMed:36871985 [IF=3.252] Ganggang Li. et al. Baicalin suppresses neuron autophagy and apoptosis by regulating astrocyte polarization in pentylenetetrazol-induced epileptic rats and PC12 cells. Brain Res. 2022 Jan;1774:147723  WB ;  Rat.   PubMed:34780748 [IF=2.826] Takanori Watanabe et al. Aquaporin 3 Expression in Endometrioid Carcinoma of the Uterine Body Correlated With Early Stage and Lower Grade. Pathol Oncol Res. 2020 Oct;26(4):2247-2253.  IHC ;  Human.   PubMed:32382899 [IF=2.51] Gang, Zhang, et al. "Detection of hypoxic-ischemic brain injury with 3D-enhanced T2* weighted angiography (ESWAN) imaging." European Journal of Radiology (2013).  IHC-P ;  Pig.   PubMed:23777745 [IF=2.02] Duan, Hubin, et al. "The role of neuropeptide Y and aquaporin 4 in the pathogenesis of intestinal dysfunction caused by traumatic brain injury." Journal of Surgical Research (2013).  IHC-P ;  Rat.   PubMed:23622727 [IF=0.64] Rajesh, Kumar, Li Xiaojie, and Kong Xiangying. "The Effect of Early Intervention and Rehabilitation in the Expression of Aquaporin-4; and Ultrastructure Changes on Rats Offsprings Damaged Brain Caused by Intrauterine Infection." Journal of Korean Neurosurgical Society 58.1 (2015): 14-21.  WB ;  Rat.   PubMed:26279808 [IF=0.23] Li, Cheng, et al. "Melatonin lowers edema after spinal cord injury." Neural Regeneration Research 9.24 (2014): 2205.  IHC-P ;  Rat.   PubMed:25657743
Research Area	Tumour  Signal transduction  Channel protein  The new supersedes the old
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Pig, Cow, Rabbit, Sheep, )
Applications	WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	36kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human AQP4: 271-323/323 <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Function: Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. Subunit: Homotetramer. Part of a complex containing MLC1, TRPV4, HEPACAM and ATP1B1. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Brain, muscle, heart, kidney, lung, and trachea. Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family. SWISS: P55087 Gene ID: 361 Database links: Entrez Gene: 281008 Cow Entrez Gene: 361 Human Entrez Gene: 11829 Mouse Entrez Gene: 25293 Rat Omim: 600308 Human SwissProt: O77750 Cow SwissProt: P55087 Human SwissProt: P55088 Mouse SwissProt: P47863 Rat Unigene: 281008 Cow Unigene: 315369 Human Unigene: 250786 Mouse Unigene: 90091 Rat Channel protein（Channel Protein） 水Channel protein(AQPs)广泛分布于机体不同组织器官中,脑组织中的水Channel protein主要为AQP4. AQP-4蛋白主要在星型胶质细胞和室管膜细胞内表达,尤其在于毛细血管和软脑膜直接接触的胶质细胞上及血管周表达丰富. 水Channel protein4的分布特点与其功能密切相关,可能是脑脊液重吸收、渗透压调节、脑水肿形成等生理、病理过程的分子生物学基础.研究水Channel protein4可为脑水肿及水代谢疾病提供分子水平的理论依据,同时为临床治疗脑水肿及水代谢疾病提供一种新途径.
Product Picture	Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AQP4 Polyclonal Antibody, Unconjugated(SL0634R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Aquaporin 4) Polyclonal Antibody, Unconjugated (SL0634R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AQP4 Polyclonal Antibody, Unconjugated(SL0634R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AQP4 Polyclonal Antibody, Unconjugated(SL0634R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (human kidney carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Aquaporin 4) Polyclonal Antibody, Unconjugated (SL0634R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control: K562 (blue). Primary Antibody:Rabbit Anti-Aquaporin 4 antibody (SL0634R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody (SL0634R, 1μg /1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min at room temperature. Acquisition of 20,000 events was performed. Blank control:Molt4. Primary Antibody (green line): Rabbit Anti-AQP 4 antibody (SL0634R-A488) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

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