Product Name	Adiponectin receptor 2
Chinese Name	脂联素受体2抗体
Alias	Adipo R2; Progestin and adipoQ receptor family member II; Adipor2; PAQR2; ADR2_HUMAN.
literatures	Specific References  (8)     |     SL0611R has been referenced in 8 publications. [IF=6.43] Morad, Vivian, Annelie Abrahamsson, and Charlotta Dabrosin. "Estradiol affects extracellular leptin: adiponectin ratio in human breast tissue in vivo." The Journal of Clinical Endocrinology & Metabolism (2014).  IHC-P ;  Human.   PubMed:24796929 [IF=5.037] Annamária Schaffer. et al. The ontogenies of endometrial and myometrial leptin and adiponectin receptors in pregnant rats: Their putative impact on uterine contractility. Life Sci. 2022 May;297:120465  WB ;  Rat.   PubMed:35271883 [IF=4.75] Guo et al. Effect of telmisartan on the expression of adiponectin receptors and nicotinamide adenine dinucleotide phosphate oxidase in the heart and aorta in type 2 diabetic rats. (2012) Cardiovasc.Diabetol. 11:94  IHC ;  Rat.   PubMed:22873349 [IF=3.776] Guoying Zhu. et al. Celecoxib-mediated attenuation of non-alcoholic steatohepatitis is potentially relevant to redistributing the expression of adiponectin receptors in rats. HELIYON. 2022 Jul;8:e09872  WB ;  Rat.   PubMed:35832345 [IF=3.54] Hsu, Ying-Jung, et al. "Effects of Fenofibrate on Adiponectin Expression in Retinas of Streptozotocin-Induced Diabetic Rats." Journal of diabetes research 2014 (2014).  WB ;   PubMed:25525608 [IF=2.59] Liu, Su, et al. "The ameliorating effect of rosiglitazone on experimental nonalcoholic steatohepatitis is associated with regulating adiponectin receptor expression in rats." European journal of pharmacology 650.1 (2011): 384.  WB ;  Rat.   PubMed:20965162 [IF=2.51] Ji, Hongfei, et al. "The effect of resveratrol on the expression of AdipoR1 in kidneys of diabetic nephropathy." Molecular Biology Reports: 1-9.  IHC-P ;  Rat.   PubMed:24413998 [IF=1.69] Zhou et al. Associations of adiponectin receptor 2 (AdipoR2) gene polymorphisms and AdipoR2 protein expression levels with the risk of colorectal cancer: A case-control study. (2017) Mol.Med.Rep. 16:3983-3993  IHC-P ;  Human.   PubMed:28765899
Research Area	Cell biology  immunology  Growth factors and hormones  The cell membrane受体  Diabetes  Endocrinopathy
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	44kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Adiponectin receptor 2: 315-340/386
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The adiponectin receptors, ADIPOR1 (MIM 607945) and ADIPOR2, serve as receptors for globular and full-length adiponectin (MIM 605441) and mediate increased AMPK (see MIM 602739) and PPAR-alpha (PPARA; MIM 170998) ligand activities, as well as fatty acid oxidation and glucose uptake by adiponectin (Yamauchi et al., 2003 [PubMed 12802337]).[supplied by OMIM, Mar 2008] Function: Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some intermediate-affinity receptor activity for both globular and full-length adiponectin. Subunit: May form homo and heteromultimers. Subcellular Location: Membrane; Multi-pass membrane protein. Note=Localized to the cell membrane and intracellular organelles. Tissue Specificity: Highly expressed in skeletal muscle, liver and placenta. Weakly expressed in brain, heart, colon, spleen, kidney, thymus, small intestine, peripheral blood leukocytes and lung. Similarity: Belongs to the ADIPOR family. SWISS: Q86V24 Gene ID: 79602 Database links: Entrez Gene: 79602 Human Entrez Gene: 68465 Mouse Entrez Gene: 404694 Pig Entrez Gene: 312670 Rat Omim: 607946 Human SwissProt: Q86V24 Human   脂联素与受体结合后具有增强胰岛素敏感性，抗高血糖，抗动脉粥样硬化等生物学效应，任何增加或减少脂联素及其受体表达的方法都影响这些疾病的发生和发展。 脂联素受体(AdipoR1和AdipoR2)同属于PAQR家族，具有七次Transmembrane protein的特有结构.
Product Picture	Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti- Adiponectin Receptor 2 (SL0611R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 44 kD Sample: Lane 1: Heart (Mouse) Lysate at 40 ug Lane 2: Skeletal muscle (Mouse) Lysate at 40 ug Lane 3: Heart (Rat) Lysate at 40 ug Lane 4: Skeletal muscle (Rat) Lysate at 40 ug Lane 5: 293T (Human) Cell Lysate at 30 ug Primary: Anti-Adiponectin receptor 2 (SL0611R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 43 kD Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti-Adiponectin receptor 2 (SL0611R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 48 kD Sample: muscle (Mouse) Lysate at 40 ug Primary: Anti-Adiponectin receptor 2 (SL0611R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 44 kD Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Adiponectin receptor 2) Polyclonal Antibody, Unconjugated (SL0611R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: human rectal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Adiponectin Receptor 2 Polyclonal Antibody, Unconjugated(SL0611R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Adiponectin Receptor 2 Polyclonal Antibody, Unconjugated(SL0611R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: HepG2(blue). Primary Antibody:Rabbit Anti-Adiponectin receptor 2 antibody (SL0611R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody (SL0611R, 0.2μg /1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. Acquisition of 20,000 events was performed.

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