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Product Name TNFRSF10A Chinese Name 死亡受体4抗体 Alias TR10A_HUMAN; Tumor necrosis factor receptor superfamily member 10A; APO2; DR4; TRAILR1; Death receptor 4; TNF-related apoptosis-inducing ligand receptor 1 (TRAIL receptor 1; TRAIL-R1); CD261; literatures Specific References (2) | SL0591R has been referenced in 2 publications.[IF=7.129] Furui Han. et al. In vivo and in vitro study on hepatotoxicity of Tris-(2, 3-dibromopropyl) isocyanurate exposure via mitochondrial and death receptor pathway. ECOTOX ENVIRON SAFE. 2022 Nov;246:114186 WB ; Rat, Human.[IF=3.752] Zhao Zhang. et al. Effect of matrine in MAC-T cells and their transcriptome analysis: A basic study. PLOS ONE. 2023 Jan;18(1):e0280905 WB ; Bovine.Research Area Cell biology immunology Immunogen Species Rabbit Clonality Polyclonal React Species Human, Applications WB=1:200-500 ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 50kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human TNFRSF10A: 401-468/468 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. [provided by RefSeq, Jul 2008]
Function:
Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B.
Subunit:
Can interact with TRADD and RIPK1. Interacts with ARAP1.
Subcellular Location:
Membrane; Single-pass type I membrane protein.
Tissue Specificity:
Widely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K562 erythroleukemia cells, MCF7 breast carcinoma cells and activated T-cells.
Similarity:
Contains 1 death domain.
Contains 3 TNFR-Cys repeats.
SWISS:
O00220
Gene ID:
8797
Database links:Entrez Gene: 8797 Human
SwissProt: O00220 Human
Product Picture Sample:
Lane 1: Human Jurkat cell lysates
Lane 2: Human A549 cell lysates
Lane 3: Human HUVEC cell lysates
Lane 4: Human HeLa cell lysates
Primary: Anti-TNFRSF10A (SL0591R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 60 kDa
Sample:
K562(Human) Cell Lysate at 40 ug
Primary: Anti- DR4 (SL0591R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 50 kD
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DR4 Polyclonal Antibody, Unconjugated(SL0591R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TRAILR1 Polyclonal Antibody, Unconjugated(SL0591R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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