Product Name	IL2RA/CD25
Chinese Name	白介素2受体a链/IL-2RA抗体
Alias	Interleukin 2 receptor alpha chain; CD 25; CD25 antigen; IDDM10; IL 2 RA; IL 2 receptor alpha subunit; IL2 RA; IL-2RA; IL2R; IL2RAC; Interleukin 2 Receptor Alpha; Interleukin 2 receptor alpha chain; Interleukin 2 receptor; Ly43; p55; T Cell Growth Factor Receptor; TAC Antigen; TCGFR; IL2RA_MOUSE.
literatures	Specific References  (4)     |     SL0577R has been referenced in 4 publications. [IF=6.073] Wen Long. et al. Gut Microbiota Protected Against pseudomonas aeruginosa Pneumonia via Restoring Treg/Th17 Balance and Metabolism. FRONT CELL INFECT MI. 2022 Jun;0:751  IF ;  Mouse.   PubMed:35782123 [IF=4.718] Arumugam P et al. Expression of a Functional IL-2 Receptor in Vascular Smooth Muscle Cells. The Journal of Immunology,2018 ji1701151.  WB ;  Human.   PubMed:doi:10.4049/jimmunol.1701151 [IF=4.372] Zhang et al. Sonodynamic therapy-assisted immunotherapy: A novel modality for cancer treatment. (2018) Cancer.Sci. 109:1330-1345  IHC ;  mouse.   PubMed:29575297 [IF=1.396] Xiong Y et al. Functions of T-cell subsets and their related cytokines in the pathological processes of autoimmune encephalomyelitic mice.(2018) Int J Clin Exp Pathol;11(10):4817-4826.  FC&IHC ;  Mouse.   PubMed:ISSN:1936-2625/IJCEP0080924
Research Area	Tumour  immunology  The cell membrane受体  lymphocyte  t-lymphocyte  b-lymphocyte
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=0.2ug /test  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	28kDa
Detection molecular weight	55kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from mouse CD25: 201-268/268
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]. Function: Receptor for interleukin-2. Subunit: Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit. Subcellular Location: Membrane; Single-pass type I membrane protein. Similarity: Contains 2 Sushi (CCP/SCR) domains. SWISS: P01590 Gene ID: 16184 Database links: Entrez Gene: 3559 Human Entrez Gene: 16184 Mouse SwissProt: P01589 Human SwissProt: P01590 Mouse Unigene: 231367 Human Unigene: 915 Mouse Omim: 147730 Human CD25抗原是分子量为55KD的单链glycoprotein，故又称白介素2受体。主要用于与淋巴样Tumour、自身免疫性疾病及同种异体移植排斥反应等疾病的研究。CD25是白介素受体的α亚单位，主要表达于活化的T细胞，在活化的B细胞、NK细胞和巨噬细胞也有一定量的表达。该抗体主要用于标记活化的lymphocyte。
Product Picture	Sample: Lymph node (Mouse) Lysate at 40 ug Primary: Anti-IL2RA (SL0577R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 28 kD Observed band size: 55 kD Blank control (Black line): Jurkat (Black). Primary Antibody (green line): Rabbit Anti-IL2RA/CD25 antibody (SL3152R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 15 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control: U937 (blue). Primary Antibody:Rabbit Anti- CD25 antibody(SL0577R), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (SL0577R, 0.2μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

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