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Product Name CCNA2 Chinese Name 周期素A2抗体 Alias CCNA2_HUMAN; Cyclin-A2; Cyclin-A; CCN1; CCN1; CCNA; cyclin A2; literatures Specific References (4) | SL0571R has been referenced in 4 publications.[IF=8.74] Chen et al. STAT1 inhibits human hepatocellular carcinoma cell growth through induction of p53 and Fbxw7. (2015) Cancer.Cell.Int. 15:111 WB ; Human.[IF=5.31] Meiju Zhang. et al. The role of E2A in ATPR-induced cell differentiation and cycle arrest in acute myeloid leukaemia cells. J Cell Mol Med. 2022 Feb;26(4):1128-1143 IHC ; Mouse.[IF=2.41] Chang et al. Analysis of the ways and methods of signaling pathways in regulating cell cycle of NIH3T3 at transcriptional level. (2015) BMC.Cell.Biol. 16:25 WB ; Mouse.[IF=2.08] Zhang, Jihong, et al. "Interleukin 18 augments growth ability via NF-κB and p38/ATF2 pathways by targeting cylin B1, cyclin B2, cylin A2, and Bcl-2 in BRL-3A rat liver cells." Gene (2015). WB ; Rat.Research Area Tumour Cardiovascular Cell biology Cyclin Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 49kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human CCNA2: 375-431/431 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene belongs to the highly conserved cyclin family, whose members function as regulators of the cell cycle. This protein binds and activates cyclin-dependent kinase 2 and thus promotes transition through G1/S and G2/M. [provided by RefSeq, Aug 2016]
Function:
Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
Subunit:
Interacts with the CDK1 and CDK2 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. When associated with CDK2 (but not with CDK1), interacts with SCAPER.
Subcellular Location:
Nucleus. Cytoplasm. Note=Cytoplasmic when associated with SCAPER.
Post-translational modifications:
Polyubiquitinated via 'Lys-11'-linked ubiquitin by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome. Deubiquitinated and stabilized by USP37 enables entry into S phase.
Similarity:
Belongs to the cyclin family. Cyclin AB subfamily.
SWISS:
P20248
Gene ID:
890
Database links:
Entrez Gene: 890 Human
Entrez Gene: 12428 Mouse
Omim: 123835 Human
SwissProt: P20248 Human
SwissProt: P51943 Mouse
Unigene: 58974 Human
Unigene: 4189 Mouse
Unigene: 13094 Rat
Cyclin A 可以出现在所有类型的细胞。各周期素可出现在不同的细胞增殖周期中。能结合和激活CDK2激酶,促进细胞G1/S和G2/M增殖周期的转化。Product Picture Tissue/cell: human bile duct cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cyclin A2 Polyclonal Antibody, Unconjugated(SL0571R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse lymphoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cyclin A2 Polyclonal Antibody, Unconjugated(SL0571R) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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