Product Name	SMAD7
Chinese Name	Smad7抗体
Alias	MAD (mothers against decapentaplegic Drosophila) homolog 7; MAD; Mad homolog 7; MAD mothers against decapentaplegic homolog 7; MADH 7; MADH 8; MADH8; Mothers Against Decapentaplegic Drosophila Homolog of 7; Mothers against decapentaplegic homolog 7; Mothers against decapentaplegic homolog 8; Mothers against DPP homolog 7; Mothers against DPP homolog 8; SMA- AND MAD-RELATED PROTEIN 7; SMAD 7; SMAD; SMAD family member 7; SMAD, mothers against DPP homolog 7 (Drosophila); SMAD, mothers against DPP homolog 7; SMAD7_HUMAN.
literatures	Specific References  (18)     |     SL0566R has been referenced in 18 publications. [IF=5.893] Ying Wang. et al. Discovery of a novel short peptide with efficacy in accelerating the healing of skin wounds. Pharmacol Res. 2021 Jan;163:105296  WB ;  Mouse.   PubMed:33220421 [IF=4.784] Zheng Wu. et al. FOXD3 suppresses epithelial–mesenchymal transition through direct transcriptional promotion of SMAD7 in esophageal squamous cell carcinoma. 2021 Sep 22  WB ;  human.   PubMed:34551139 [IF=4.171] Yi Chen. et al. The essential oil from the raw and vinegar processed Rhizoma Curcumae ameliorate CCl4-incuded liver fibrosis: integrating network pharmacology and molecular mechanism evaluation. 2021 Mar 17  WB ;  Rat.   PubMed:33870974 [IF=4.101] Yu Guo. et al. RepSox effectively promotes the induced differentiation of sheep fibroblasts into adipocytes via the inhibition of the TGF‑β1/Smad pathway. Int J Mol Med. 2021 Aug;48(2):1-13  WB ;  Sheep.   PubMed:34132357 [IF=3.887] Chengshan JIN. et al. Qianjin Wenwu decoction suppresses renal interstitial fibrosis by enhancing the degradation of extracellular matrix in mice with unilateral ureteral obstruction. CHIN J NAT MEDICINES. 2023 Apr;21:253  WB ;  Mouse.   PubMed:37120244 [IF=3.66] Wang et al. Effects of Tongxinluo on myocardial fibrosis in diabetic rats. (2016) J.Chin.Med.Assoc. 79:130-6  IHC ;  Rat.   PubMed:26775604 [IF=3.571] Zheng HX et al. Cyanidin-3-glucoside from Black Rice Ameliorates Diabetic Nephropathy via Reducing Blood Glucose, Suppressing Oxidative Stress and Inflammation, and Regulating Transforming Growth Factor β1/Smad Expression. J Agric Food Chem. 2020 Apr 15;68(15):4399-4410.  IHC-P ;  Rat.   PubMed:32192334 [IF=3.457] Gao H et al.Salvanic acid B inhibits myocardial fibrosis through regulating TGF-β1/Smad signaling pathway.(2018) Biomed. Pharmacother 110  IHC-P&WB ;  Mouse.   PubMed:30553195 [IF=3.204] Chi Li. et al. Schisantherin D from Schisandra chinensis (Turcz.) Baill. exhibits anti-liver fibrosis capacity via modulating ETBR involved signaling, an in vitro and in vivo study. FITOTERAPIA. 2022 Oct;162:105290  WB ;  Mouse, Human.   PubMed:36064152 [IF=2.923] Taojie Zhang. et al. MEL regulates miR-21 and let-7b through the STAT3 cascade in the follicular granulosa cells of Tibetan sheep. THERIOGENOLOGY. 2023 Apr;:  WB ;  Sheep.   PubMed:37120893 [IF=2.31] Liu, Wei, et al. "Synchronous alteration pattern between Serine‐Threonine kinase receptor associated protein and Smad7 in pilocarpine‐induced rats of epilepsy." Synapse (2014).  WB ;  Rat.   PubMed:24577865 [IF=2.067] H-J Wang. et al. MiR-32-5p knockdown inhibits epithelial to mesenchymal transition and renal fibrosis by targeting SMAD7 in diabetic nephropathy:. Hum Exp Toxicol. 0;():  WB ;  Human.   PubMed:32959695 [IF=2.014] Sheng-Nan ZHOU. et al. Early intervention with Di-Dang Decoction prevents macrovascular fibrosis in diabetic rats by regulating the TGF-β1/Smad signalling pathway. Chin J Nat Medicines. 2020 Aug;18:612  WB ;  Rat.   PubMed:10.1016/S1875-5364(20)30073-X [IF=1.974] Taojie Zhang. et al. miR-21, miR-125b, and let-7b contribute to the involution of atretic follicles and corpus lutea in Tibetan sheep ovaries. ANIM SCI J. 2022 Jan;93(1):e13756  WB ;  Sheep.   PubMed:35822516 [IF=1.96] Kou, Wei, et al. "Transforming growth factor-β1 promotes Treg commitment in nasal polyposis after intranasal steroid treatment." Inflammation Research (2013): 1-7.  ELISA ;  Human.   PubMed:23178794 [IF=1.66] Xiaodong Zhang. et al. MiR-21-5p actions at the Smad7 gene during pig ovarian granulosa cell apoptosis. Anim Reprod Sci. 2020 Dec;223:106645  WB,IF ;  Pig.   PubMed:33217624 [IF=1.56] Yang, Yang, et al. "MicroRNA-15a inhibition protects against hypoxia/reoxygenation-induced apoptosis of cardiomyocytes by targeting mothers against decapentaplegic homolog 7." Molecular Medicine Reports (2017).  WB ;  Rat.   PubMed:28440490 [IF=1.396] Zhang Z et al. Centella asiatica inhibits renal interstitial fibrosis by regulating Smad3 and Smad7 expression in the TGFβ signaling pathway.  IHC-P&WB ;  Rat.   PubMed:ISSN:1936-2625/IJCEP0071460
Research Area	Tumour  Cell biology  Signal transduction  Apoptosis  Growth factors and hormones  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Pig, Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	46kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Smad7: 1-100/426
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] Function: Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. Subunit: Interacts with WWP1. Interacts with COPS5. Interacts with NEDD4L. Interacts with STAMBP. Interacts with RNF111, AXIN1 and AXIN2. Interacts with PPP1R15A. Interacts (via MH2 domain) with EP300. Interacts with ACVR1B, SMURF1, SMURF2 and TGFBR1; SMAD7 recruits SMURF1 and SMURF2 to the TGF-beta receptor and regulates its degradation. Interacts with PDPK1 (via PH domain). Subcellular Location: Nucleus. Cytoplasm. Note=Interaction with NEDD4L or RNF111 or induces translocation from the nucleus to the cytoplasm. TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta. Tissue Specificity: Ubiquitous with higher expression in the lung and vascular endothelium. Post-translational modifications: Phosphorylation on Ser-249 does not affect its stability, nuclear localization or inhibitory function in TGFB signaling; however it affects its ability to regulate transcription. Phosphorylated by PDPK1. Ubiquitinated by WWP1 (By similarity). Polyubiquitinated by RNF111, which is enhanced by AXIN1 and promotes proteasomal degradation. In response to TGF-beta, ubiquitinated by SMURF1; which promotes its degradation. Acetylation prevents ubiquitination and degradation mediated by SMURF1. DISEASE: Genetic variations in SMAD7 influence susceptibility to colorectal cancer type 3 (CRCS3) [MIM:612229]. Colorectal cancer consists of tumors or cancer of either the colon or rectum or both. Cancers of the large intestine are the second most common form of cancer found in males and females. Symptoms include rectal bleeding, occult blood in stools, bowel obstruction and weight loss. Treatment is based largely on the extent of cancer penetration into the intestinal wall. Surgical cures are possible if the malignancy is confined to the intestine. Risk can be reduced when following a diet which is low in fat and high in fiber. Similarity: Belongs to the dwarfin/SMAD family. Contains 1 MH1 (MAD homology 1) domain. Contains 1 MH2 (MAD homology 2) domain. SWISS: O15105 Gene ID: 4092 Database links: Entrez Gene: 4092 Human Entrez Gene: 17131 Mouse Entrez Gene: 81516 Rat Omim: 602932 Human SwissProt: O15105 Human SwissProt: O35253 Mouse SwissProt: O88406 Rat Unigene: 465087 Human transcriptional regulatory factor（Transcriptin Regulators） Smad7是转化生长因子(TGF-β)信号通路的抑制分子，Smad7可干预MAPK信号通路,使ERK和JNK磷酸化活性的平衡失调,导致促增殖作用强于生长抑制作用,从而有助于细胞向恶性方向发展。
Product Picture	Sample: Lung(Mouse) Lysate at 30 ug Placenta(Mouse) Lysate at 30 ug Large intestine(Mouse) Lysate at 30 ug Primary: Anti- MADH7/Smad7 (SL0566R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 50 kD Sample: Lane 1: Stomach (Mouse) Lysate at 40 ug Lane 2: Spleen (Mouse) Lysate at 40 ug Lane 3: Lung (Mouse) Lysate at 40 ug Primary: Anti-MADH7/Smad7 (SL0566R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Cerebrum (Rat) Lysate at 40 ug Lane 3: Stomach (Mouse) Lysate at 40 ug Lane 4: Lung (Mouse) Lysate at 40 ug Lane 5: Kidney (Mouse) Lysate at 40 ug Primary: Anti-MADH7/Smad7 (SL0566R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MADH7) Polyclonal Antibody, Unconjugated (SL0566R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MADH7) Polyclonal Antibody, Unconjugated (SL0566R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MADH7) Polyclonal Antibody, Unconjugated (SL0566R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Smad7) Polyclonal Antibody, Unconjugated (SL0566R) at 1:600 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Smad7/Smad6 Polyclonal Antibody, Unconjugated(SL0071R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining U-2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (MADH7/Smad7) polyclonal Antibody, Unconjugated (SL0566R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control: SH-SY5Y. Primary Antibody (green line): Rabbit Anti-MADH7/Smad7 antibody (SL0566R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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