Product Name	MDR1
Chinese Name	多药耐药蛋白/P-glycoprotein抗体
Alias	P-Glycoprotein; Multi Drug Resistance Associated Protein, ABCB1; ATP-binding cassette sub-family B (MDR/TAP), member 1; ABC20; CD243; CD243 antigen; CLCS; Multidrug resistance protein 1; P glycoprotein 1; p-GP; gp170; P gp; PGY1; MDR1_HUMAN.
literatures	Specific References  (13)     |     SL0563R has been referenced in 13 publications. [IF=9.727] Hiroshi Kikuchiet al. Chemotherapy-Induced IL8 Upregulates MDR1/ABCB1 in Tumor Blood Vessels and Results in Unfavorable Outcome. Cancer Res . 2020 Jul 15;80(14):2996-3008.  other ;   PubMed:32536602 [IF=7.149] Yuan P et al. WS2 Nanosheets at Noncytotoxic Concentrations Enhance the Cytotoxicity of Organic Pollutants by Disturbing the Plasma Membrane and Efflux Pumps. Environ Sci Technol. 2020 Feb 4;54(3):1698-1709.  WB ;  Mouse&Human.   PubMed:31916439 [IF=6.93] Peng, Lei, et al. "Development of a novel orthotopic non-small cell lung cancer model and therapeutic benefit of 2’-(2-bromohexadecanoyl)-docetaxel conjugate nanoparticles." Nanomedicine: Nanotechnology, Biology and Medicine (2014).  IHC-P ;  Mouse.   PubMed:24709328 [IF=4.357] Wei et al. The impact of insulin on chemotherapeutic sensitivity to 5-fluorouracil in gastric cancer cell lines SGC7901, MKN45 and MKN28. (2015) J.Exp.Clin.Cancer.Re. 34:64  WB ;  Human.   PubMed:26084465 [IF=3.779] Kai-Fu Chang. et al. Cedrol, a Sesquiterpene Alcohol, Enhances the Anticancer Efficacy of Temozolomide in Attenuating Drug Resistance via Regulation of the DNA Damage Response and MGMT Expression. J Nat Prod. 2020;83(10):3021–3029  WB ;  Rat.   PubMed:32960603 [IF=3.33] Chen et al. Timosaponin A-III reverses multi-drug resistance in human chronic myelogenous leukemia K562/ADM cells via downregulation of MDR1 and MRP1 expression by inhibiting PI3K/Akt signaling pathway. (2016) Int.J.Oncol. 48:2063-70  WB ;  Human.   PubMed:26984633 [IF=3.223] Chi-Yen Liang. et al. Patchouli alcohol induces G0/G1 cell cycle arrest and apoptosis in vincristine-resistant non-small cell lung cancer through ROS-mediated DNA damage. THORAC CANCER. 2023 May;:  ICC,WB ;  Human.   PubMed:37249164 [IF=3.07] Wen-Qing, Hu, Peng Chun-Wei, and Li Yan. "The expression and significance of P-glycoprotein, lung resistance protein and multidrug resistance-associated protein in gastric cancer." Journal of Experimental & Clinical Cancer Research 28 (2009).  Human.   PubMed:19930704 [IF=2.969]  Xue Zhou . et al. Let-7b regulates the adriamycin resistance of chronic myelogenous leukemia by targeting AURKB in K562/ADM cells. Leukemia Lymphoma. 2020;61(14):3451-3459  WB ;  Human.   PubMed:32856506 [IF=2.663] Liu QH et al. Reduced expression of annexin A1 promotes gemcitabine and 5-fluorouracil drug resistance of human pancreatic cancer. Invest New Drugs. 2019 May 23.  WB ;  Human.   PubMed:31124054 [IF=2.502] Sun et al. MicroRNA-186 induces sensitivity of ovarian cancer cells to paclitaxel and cisplatin by targeting ABCB1. (2015) J.Ovarian.Re. 8:80  WB ;  Human.   PubMed:26626440 [IF=2.389] Huang Weizhen. et al. LncRNA SNHG11 enhances bevacizumab resistance in colorectal cancer by mediating miR-1207-5p/ABCC1 axis. ANTI-CANCER DRUG. 2022 Jul;33(6):575-586  WB ;  Human.   PubMed:35324517 [IF=2.311] Haruna Makita. et al. Xenografts derived from patients with head and neck cancer recapitulate patient tumour properties. Oncol Lett. 2021 May;21(5):1-10  IHC ;  Human.   PubMed:33777208
Research Area	Tumour  Cardiovascular  Cell biology  immunology  Signal transduction  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	141kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human MDR1: 21-100/1272 <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	P Glycoprotein, the product of the MDR1 gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of P Glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P Glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P Glycoprotein have also been reported in multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas and leukemias / lymphomas. Function: Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. Subunit: Interacts with PSMB5. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in liver, kidney, small intestine and brain. DISEASE: Genetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases. Similarity: Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains. SWISS: P21447 Gene ID: 5243 Database links: Entrez Gene: 5243 Human Entrez Gene: 18669 Mouse Entrez Gene: 170913 Rat Entrez Gene: 24646 Rat Omim: 171050 Human SwissProt: P08183 Human SwissProt: P06795 Mouse SwissProt: P43245 Rat
Product Picture	Sample: Liver (mouse) Lysate at 40 ug Primary: Anti- MDR1 (SL0563R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 141kD Observed band size: 150kD Sample: Lane 1: Liver (Mouse) Lysate at 40 ug Lane 2: SH-SY5Y (Human) Cell Lysate at 30 ug Lane 3: HepG2 (Human) Cell Lysate at 30 ug Lane 4: 293T (Human) Cell Lysate at 30 ug Primary: Anti-MDR1 (SL0563R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 150-180 kD Observed band size: 150 kD Tissue/cell:SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (MDR1) polyclonal Antibody, Unconjugated (SL0563R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Tissue/cell:SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (MDR1) polyclonal Antibody, Unconjugated (SL0563R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control (blue line): Hela (fixed with 70% methanol (Overnight at -20℃) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-MDR1 antibody (SL0563R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.

Cartpieces

• 
• 1

  1Delete

• 
• 5

  5Delete

• 
• S

  SDelete

• 
• R

  RDelete

• 
• +

  +Delete

• 
• 7

  7Delete

• Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• P

  PDelete

• 
• P

  PDelete

• 

• Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• 
• t

  tDelete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• R

  RDelete

• 
• 5

  5Delete

• 
• A

  ADelete

Totalgoods,subtotals:￥Checkout