Product Name	IL18
Chinese Name	白细胞介素-18/Interferonγ诱导因子抗体
Alias	Interleukin-18; interferon gamma-inducing factor IGIF; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; Iboctadekin; IFN gamma inducing factor; IGIF; IL 1 gamma; IL 18; IL-18; IL 1g; IL1 gamma; IL18 protein; IL1F4; IL1g; IL1gamma; ILIF4; Interferon gamma inducing factor; Interleukin 1 gamma; Interleukin18; MGC12320; IL18_MOUSE.
literatures	Specific References  (26)     |     SL0529R has been referenced in 26 publications. [IF=16.907] Yunxiang Sun. et al. Spontaneous formation of β-sheet nano-barrels during the early aggregation of Alzheimer’s amyloid beta. Nano Today. 2021 Jun;38:101125  WB ;  Mouse.   PubMed:10.1016/j.nantod.2021.101125 [IF=9.811] Nicholas Andrikopoulos. et al. Inhibition of Amyloid Aggregation and Toxicity with Janus Iron Oxide Nanoparticles. Chem Mater. 2021;XXXX(XXX):XXX-XXX  WB ;  Mouse.   PubMed:10.1021/acs.chemmater.1c01947 [IF=9.043] Jiayu Yu. et al. Disruption of the Intestinal Mucosal Barrier Induced by High Fructose and Restraint Stress Is Regulated by the Intestinal Microbiota and Microbiota Metabolites. MICROBIOL SPECTR. 2023 Jan 31  WB ;  Mouse.   PubMed:36719201 [IF=8.025] Lihui Ni. et al. The combination of insulin and linezolid ameliorates Staphylococcus aureus pneumonia in individuals with diabetes via the TLR2/MAPKs/NLRP3 pathway. INT J BIOL MACROMOL. 2023 Jul;242:124750  WB ;  Mouse.   PubMed:37160172 [IF=7.129] Ruxue Jia. et al. PM2.5 induce myocardial injury in hyperlipidemic mice through ROS-pyroptosis signaling pathway. ECOTOX ENVIRON SAFE. 2023 Apr;254:114699  WB ;  Mouse.   PubMed:36889212 [IF=6.291] Shaofeng Wu. et al. The neuroprotective effect of curcumin against ATO triggered neurotoxicity through Nrf2 and NF-κB signaling pathway in the brain of ducks. Ecotox Environ Safe. 2021 Dec;228:112965  WB ;  Duck.   PubMed:34775344 [IF=5.793] Yuan Changet al. NLRP3 inflammasome-mediated microglial pyroptosis is critically involved in the development of post-cardiac arrest brain injury. J Neuroinflammation . 2020 Jul 23;17(1):219.  WB ;  rat.   PubMed:32703306 [IF=5.62] Weijian Fan. et al. RGD1564534 represses NLRP3 inflammasome activity in cerebral injury following ischemia-reperfusion by impairing miR-101a-3p-mediated Dusp1 inhibition. EXP NEUROL. 2022 Nov;:114266  WB ;  Rat.   PubMed:36336032 [IF=4.691] Xiaoli Liu. et al. Bone marrow mesenchymal stem cell-derived exosomes attenuate cerebral ischemia-reperfusion injury-induced neuroinflammation and pyroptosis by modulating microglia M1/M2 phenotypes. Exp Neurol. 2021 Mar;:113700  WB ;  Rat.   PubMed:33741350 [IF=4.527] Gao M et al. Is NLRP3 or NLRP6 inflammasome activation associated with inflammation-related lung tumorigenesis induced by benzo (a) pyrene and lipopolysaccharide? Ecotoxicol Environ Saf. 2019 Sep 24;185:109687.  IHC ;  Mouse.   PubMed:31561077 [IF=3.943] Mengli Cui et al. NLRP3 inflammasome is involved in nerve recovery after sciatic nerve injury. Int Immunopharmacol. 2020 Jul;84:106492.  WB ;  Mouse.   PubMed:32402947 [IF=3.921] Meiyun Cai. et al. Kaemperfol alleviates pyroptosis and microglia-mediated neuroinflammation in Parkinson's disease via inhibiting p38MAPK/NF-κB signaling pathway. Neurochem Int. 2021 Nov;:105221  WB ;  Rat.   PubMed:34780806 [IF=3.869] Kong Dejun. et al. IL-37 Gene Modification Enhances the Protective Effects of Mesenchymal Stromal Cells on Intestinal Ischemia Reperfusion Injury. Stem Cells Int. 2020;2020:8883636  WB ;  Rat.   PubMed:32849879 [IF=3.758] Lei Xianghong. et al. Micheliolide Attenuates Lipopolysaccharide-Induced Inflammation by Modulating the mROS/NF-κB/NLRP3 Axis in Renal Tubular Epithelial Cells. Mediat Inflamm. 2020;2020:3934769  WB ;  Rat.   PubMed:32879619 [IF=3.553] Wu Z et al. Pyroptosis engagement and bladder urothelial cells derived exosomes recruit mast cells and induce barrier dysfunction of bladder urothelium after UPEC infection. Am J Physiol Cell Physiol. 2019 Jun 26.  WB ;  Mouse.   PubMed:31241987 [IF=3.188] Bian X et al. Periostin contributes to renal and cardiac dysfunction in rats with chronic kidney disease: reduction of PPARα.Biochimie. 2019 May;160:172-182.  WB ;  Rat.   PubMed:30890453 [IF=3.105] Xiao-ling Zhang. et al. Anti-inflammatory and neuroprotective effects of natural cordycepin in rotenone-induced PD models through inhibiting Drp1-mediated mitochondrial fission. Neurotoxicology. 2021 May;84:1  WB ;  Rat.   PubMed:33549657 [IF=3.063] Liu T et al. MicroRNA-193b-3p regulates hepatocyte apoptosis in selenium-deficient broilers by targeting MAML1.J Inorg Biochem. 2018 Sep;186:235-245.  WB ;  broiler chick.   PubMed:29990747 [IF=2.649] Shao H et al. Glyburide attenuates ozone‐induced pulmonary inflammation and injury by blocking the NLRP3 inflammasome. Environ Toxicol. 2020 Mar 13.  IHC ;  mouse.   PubMed:32167222 [IF=2.649] Duan S et al. NLRP3 inflammasome activation is associated with PM2. 5‐induced cardiac functional and pathological injury in mice. Environ Toxicol. 2019 Jul 16.  IHC ;  Mouse.   PubMed:31313453 [IF=2.615] Wei Yu. et al. Curcumin suppresses doxorubicin-induced cardiomyocyte pyroptosis via a PI3K/Akt/mTOR-dependent manner. Cardiovasc Diagn The. 2020 Aug; 10(4): 752–769  WB ;  Mouse.   PubMed:32968631 [IF=2.479] Wangyu Wu. et al. Cholecalciferol pretreatment ameliorates ischemia/reperfusion-induced acute kidney injury through inhibiting ROS production, NF-κB pathway and pyroptosis. Acta Histochem. 2022 May;124:151875  IF ;  Mouse.   PubMed:35334282 [IF=1.034] Yuyang Li. et al. Porphyromonas gingivalis lipopolysaccharide affects oral epithelial connections via pyroptosis. J Dent Sci. 2021 Jan;:  IHC ;  Human.   PubMed:10.1016/j.jds.2021.01.003 [IF=0.737] Yang S. Y.. et al. Exosomes Derived from Endothelial Cells Inhibit Neointimal Hyperplasia Induced by Carotid Artery Injury in Rats via ROS-NLRP3 Inflammasome Pathway. B EXP BIOL MED+. 2023 May;:1-6  WB ;  Rat.   PubMed:37162629 [IF=0.439] Chen JW et al. Zhihuang Tongfeng decoction ameliorates gouty arthritis via inhibition of NLRP3 inflammasome in rats. Tropical Journal of Pharmaceutical Research December 2019; 18 (12): 2577-2582.  WB ;  Rat.   PubMed:doi:10.4314/tjpr.v18i12.17 [IF=0] Kong D et al. IL-37 Gene Modification Enhances the Protective Effects of Mesenchymal Stromal Cells on Intestinal Ischemia Reperfusion Injury. Research Square. 17 February 2020.  WB ;  Rat.   PubMed:DOI:10.21203/rs.2.23662/v1
Research Area	immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	22kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from mouse IL-18: 51-120/192
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	IL18 (IFN-gamma-inducing factor, IGIF) is a novel pro-inflammatory cytokine that augments natural killer (NK) activity in spleen cells. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL18 also has other biological properties such as stimulation of Fas ligand expression, and induction of CC and CXC chemokines. IL18 increases production of competent HIV. IL18 is related to the IL1 family. One of the IL18 receptor chains is the IL1 receptor-related protein, a member of the IL 1R family. Like IL1 , IL18 is first synthesized as a leaderless precursor requiring the IL1 converting enzyme (ICE) for cleavage into an active molecule. IL18 may play a role in tissue injury in inflammatory reactions. Function: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. Subcellular Location: Secreted. Similarity: Belongs to the IL-1 family. SWISS: P70380 Gene ID: 16173 Database links: Entrez Gene: 3606 Human Entrez Gene: 16173 Mouse Omim: 600953 Human SwissProt: Q14116 Human SwissProt: P70380 Mouse Unigene: 83077 Human Unigene: 1410 Mouse IL-18是巨噬细胞分泌的一个重要因子，它具有多种免疫功能，能诱导产生ＩＦＮγ，无论在病毒感染早期或特异性细胞免疫应答效应阶段都会出现，因此IL-18主要参与免疫应答及炎性细胞浸润的改变。Interleukin-18 又称inteferon-gamma inducing factor(IGIF)，在辅助T细胞的应答反应中起重要作用。IL-18和IL-1一起，既参与先天免疫，又是后天免疫的构成因素。
Product Picture	Sample:Eye (Mouse) Lysate at 40 ug Primary: Anti-IL-18 (SL0529R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 18 kD Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-18) Polyclonal Antibody, Unconjugated (SL0529R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-18) Polyclonal Antibody, Unconjugated (SL0529R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: rat retina tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IL-18 Polyclonal Antibody, Unconjugated(SL0529R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IL-18 Polyclonal Antibody, Unconjugated(SL0529R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Cartpieces

• 
• 1

  1Delete

• 
• 5

  5Delete

• 
• S

  SDelete

• 
• R

  RDelete

• 
• +

  +Delete

• 
• 9

  9Delete

• Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• I

  IDelete

• 
• I

  IDelete

• 

• Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• 
• t

  tDelete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• R

  RDelete

• 
• 5

  5Delete

• 
• A

  ADelete

Totalgoods,subtotals:￥Checkout