Product Name	PKA alpha + beta
Chinese Name	蛋白激酶A抗体
Alias	PRKACA/PRKACB; PRKACA + PRKACB; PKA alpha + beta; PKA alpha; PKA beta; cAMP dependent protein kinase alpha catalytic subunit; cAMP dependent protein kinase beta catalytic subunit; cAMP dependent protein kinase catalytic subunit alpha; cAMP dependent protein kinase catalytic subunit beta; DKFZp781I2452; MGC102831; MGC41879; MGC48865; MGC9320; PKA C alpha; PKA C beta; PKACA; PKACB; PRKACA; PRKACB; Protein kinase A catalytic subunit alpha; Protein kinase A catalytic subunit; Protein kinase A catalytic subunit beta; Protein kinase cAMP dependent catalytic alpha; Protein kinase cAMP dependent catalytic beta; cAMP dependent protein kinase catalytic subunit alpha isoform 1; cAMP-dependent protein kinase catalytic subunit alpha; KAPCA_HUMAN; KAPCB_HUMAN; PKA C alpha; PKA C-alpha; PKACA; PRKACA; Protein kinase A catalytic subunit; Protein kinase cAMP dependent catalytic alpha.
literatures	Specific References  (11)     |     SL0520R has been referenced in 11 publications. [IF=6.317] Zhongxin Li. et al. Duodenal infusion of soy protein hydrolysate activates cAMP signaling and hypothalamic neurotransmitter synthesis in pigs. FOOD FUNCT. 2022 Nov;:  WB ;  Pig.   PubMed:36345723 [IF=6.317] Shiqing Sun. et al. Pharmacodynamic structure of deer antler base protein and its mammary gland hyperplasia inhibition mechanism by mediating Raf-1/MEK/ERK signaling pathway activation. FOOD FUNCT. 2023 Mar;:  WB ;  Rat.   PubMed:36939833 [IF=5.893] Yongxiang Li. et al. Food reward depends on TLR4 activation in dopaminergic neurons. Pharmacol Res. 2021 Jul;169:105659  WB ;  Mouse.   PubMed:33971268 [IF=5.58] Zhou, Zhiwen, et al. "Photoactivated adenylyl cyclase (PAC) reveals novel mechanisms underlying cAMP-dependent axonal morphogenesis." Scientific Reports 5 (2016): 19679.  Rat.   PubMed:26795422 [IF=4.2] Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).  IHC-P ;  Mouse.   PubMed:29202267 [IF=3.95] Yao, Gaoyi, et al. "Chronic SO 2 inhalation above environmental standard impairs neuronal behavior and represses glutamate receptor gene expression and memory-related kinase activation via neuroinflammation in rats." Environmental Research 137 (2015): 85-93.  WB ;  Rat.   PubMed:25498917 [IF=3.73] Haolong, Du, et al. "Enterovirus 71 VP1 Activates Calmodulin-Dependent Protein Kinase II and Results in the Rearrangement of Vimentin in Human Astrocyte Cells." PLoS One 8(9): e73900  WB ;  Human.   PubMed:24073199 [IF=3.411] Tong Hongxuan. et al. Molecular Mechanism of Tetramethylpyrazine Ameliorating Neuroexcitotoxicity through Activating the PKA/CREB Signaling Pathway. Biomed Res Int. 2022;2022:2812839  WB ;  Rat.   PubMed:35097116 [IF=3.173] Zuo D et al. Alcohol aggravates ketamine-induced behavioral, morphological and neurochemical alterationsin adolescent rats: The involvement of CREB-related pathways.Behav Brain Res. 2018 Sep 3;349:80-90.  WB ;  Rat.   PubMed:29738804 [IF=2.65] Sitong Ming. et al. Protective Effect of Shengmaiyin in Myocardial Hypertrophy-Induced Rats: A Genomic Analysis by 16S rDNA. EVID-BASED COMPL ALT. 2022 Sep 07;2022:3188292  WB ;  Rat.   PubMed:36118100 [IF=1.785] Yang Liuet al. Lidocaine improves cerebral ischemia-reperfusion injury in rats through cAMP/PKA signaling pathway. Exp Ther Med . 2020 Jul;20(1):495-499.  WB ;  rat.   PubMed:32509019
Research Area	Cell biology  Signal transduction  Apoptosis  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	40kDa
Cellular localization	The nucleus cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PRKACA/PRKACB : 17-100/351
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] Function: Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA, TRPC1 and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca(2+), leading to reduced amplitude and increased frequency of store overload-induced Ca(2+) release (SOICR) characterized by an increased rate of Ca(2+) release and propagation velocity of spontaneous Ca(2+) waves, despite reduced wave amplitude and resting cytosolic Ca(2+). TRPC1 activation by phosphorylation promotes Ca(2+) influx, essential for the increase in permeability induced by thrombin in confluent endothelial monolayers. PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Regulates negatively tight junction (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Isoform 2 phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT). Phosphorylates APOBEC3G and AICDA. Subunit: A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. The cAMP-dependent protein kinase catalytic subunit binds PJA2. Both isoforms 1 and 2 forms activate cAMP-sensitive PKAI and PKAII holoenzymes by interacting with regulatory subunit (R) of PKA, PRKAR1A/PKR1 and PRKAR2A/PKR2, respectively. Interacts with NFKB1, NFKB2 and NFKBIA in platelets; these interactions are disrupted by thrombin and collagen. Binds to ABL1 in spermatozoa and with CDC25B in oocytes. Interacts with APOBEC3G and AICDA. Subcellular Location: Cytoplasm. Cell membrane. Nucleus. Mitochondrion. Note=Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm. Distributed throughout the cytoplasm in meiotically incompetent oocytes. Associated to mitochondrion as meiotic competence is acquired. Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes. Tissue Specificity: Isoform 1 is ubiquitous. Isoform 2 is sperm specific. Post-translational modifications: Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively. Autophosphorylated. Phosphorylation is enhanced by vitamin K(2). Phosphorylated on threonine and serine residues. Phosphorylation on Thr-198 is required for full activity. Phosphorylated at Tyr-331 by activated receptor tyrosine kinases EGFR and PDGFR; this increases catalytic efficienncy. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. SWISS: P17612 Gene ID: 5566 Database links: Entrez Gene: 5566 Human Entrez Gene: 5567 Human Entrez Gene: 18747 Mouse Entrez Gene: 18749 Mouse Entrez Gene: 25636 Rat Omim: 176892 Human Omim: 601639 Human SwissProt: P17612 Human SwissProt: P22694 Human SwissProt: P05132 Mouse SwissProt: P05206 Mouse SwissProt: P27791 Rat Unigene: 487325 Human Unigene: 631630 Human Unigene: 16766 Mouse Unigene: 19111 Mouse Unigene: 103828 Rat Kinases and Phosphatases（Kinases and Phosphatases） 蛋白激酶 A (protein kinase A，PKA) 又称依赖于cAMP的蛋白激酶A (cyclic-AMP dependent protein kinase A)，是一种结构最简单、生化特性最清楚的蛋白激酶。 蛋白激酶A属于多功能丝氨酸和苏氨酸激酶,可使蛋白磷酸化，也可自身磷酸化。在调节细胞存活中起重要作用。
Product Picture	Sample: Jurkat(Human) Cell Lysate at 40 ug Primary: Anti-PKA alpha + beta (SL0520R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 40 kD Sample: Lane 1: Mouse Cerebellum tissue lysates Lane 2: Mouse Heart tissue lysates Primary: Anti-PKA alpha + beta (SL0520R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti- Rabbit IgG at 1/20000 dilution Predicted band size: 40 kDa Observed band size: 45 kDa Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PKA alpha + beta) polyclonal Antibody, Unconjugated (SL0520R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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