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Product Name IFN gamma Chinese Name Interferon-γ/IFN-γ抗体 Alias IFNG; IFG; IFI; IFN Gamma; IFN Immune; IFN-gamma; IFNG; IFNG_MOUSE; Immune Interferon; Interferon gamma; Interferon Gamma Precursor; Macrophage Activating Factor; MAF; T Cell Interferon; Type II Interferon. literatures Specific References (70) | SL0480R has been referenced in 70 publications.Research Area Tumour immunology Signal transduction Interferon Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 15kDa Cellular localization Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from mouse IFN gamma: 75-155/155 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.
Function:
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Subunit:
Homodimer.
Subcellular Location:
Secreted.
Tissue Specificity:
Released primarily from activated T lymphocytes.
Post-translational modifications:
Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
DISEASE:
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
Similarity:
Belongs to the type II (or gamma) interferon family.
SWISS:
P01580
Gene ID:
15978
Database links:
Entrez Gene: 3458 Human
Entrez Gene: 15978 Mouse
Omim: 147570 Human
SwissProt: P01579 Human
SwissProt: P01580 Mouse
Unigene: 856 Human
Unigene: 240327 Mouse
Product Picture Sample:
Disease Lung (Mouse) Lysate at 30 ug
Disease Spleen (Mouse) lysate at 30 ug
Primary: Anti- IFN gamma (SL0480R) at 1/200 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/3000 dilution
Predicted band size: 15 kD
Observed band size: 18 kD
Sample:
Lane 1: Recombinant human IFN Gamma protein, N-His Tag
Primary: Anti-IFN gamma (SL0480R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 16 kDa
Sample:
Lane 1: Mouse Raw264.7-PMA Lysates
Lane 2: Mouse Lymph Lysates
Lane 3: Mouse Spleen Lysates
Lane 4: Mouse Thymus Lysates
Lane 5: Rat Lymph Lysates
Lane 6: Rat Thymus Lysates
Lane 7: Human Jurkat-TPA cell Lysates
Lane 8: Human Jurkat cell Lysates
Lane 9: Human HepG2 cell Lysates
Lane 10: Recombinant human IFN gamma Protein (SL0388P)
Primary: Anti-IFN gamma (SL0480R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15kDa
Observed band size: 25kDa
Tissue/cell: Mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IFN gamma Polyclonal Antibody, Unconjugated(SL0480R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IFN-gamma Polyclonal Antibody, Unconjugated(SL0480R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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