Product Name	Adiponectin
Chinese Name	脂联素抗体
Alias	30 kDa adipocyte complement related protein; 30 kDa adipocyte complement-related protein; ACDC; ACRP 30; ACRP30; ADIPO_HUMAN; Adipocyte; Adipocyte C1q and collagen domain containing protein; Adipocyte complement related 30 kDa protein; Adipocyte complement related protein of 30 kDa; Adipocyte complement-related 30 kDa protein; Adiponectin; AdipoQ; Adipose most abundant gene transcript 1; Adipose most abundant gene transcript 1 protein; Adipose specific collagen like factor; ADIPQTL1; ADPN; APM 1; apM-1; ApM1; C1q and collagen domain-containing protein; GBP 28; GBP28; Gelatin binding protein; Gelatin binding protein 28; Gelatin-binding protein.
literatures	Specific References  (11)     |     SL0471R has been referenced in 11 publications. [IF=5.116] Pengyu Hong. et al. Therapeutic potential of small extracellular vesicles derived from lipoma tissue in adipose tissue regeneration—an in vitro and in vivo study. Stem Cell Res Ther. 2021 Dec;12(1):1-13  IHC ;  Human.   PubMed:33789709 [IF=4.522] Khan R et al. Bta‐miR‐149‐5p inhibits proliferation and differentiation of bovine adipocytes through targeting CRTCs at both transcriptional and posttranscriptional levels. J Cell Physiol. 2020 Jan 31.  ICF ;  Bovine.   PubMed:32003022 [IF=3.752] Chungho Lee. et al. Disturbance of lipid metabolism in germ-free mice transplanted with gut microbiota of DSS-induced colitis mice. PLOS ONE. 2023 Feb;18(2):e0280850  WB ;  Mouse.   PubMed:36735734 [IF=3.701] Yanna Huanget al. Resveratrol increase the proportion of oxidative muscle fiber through the AdipoR1-AMPK-PGC-1α pathway in pigs. Journal of Functional Foods 73 (2020) 104090  WB ;  pig.   PubMed:10.1016/j.jff.2020.104090 [IF=3.68] Du et al. MicroRNA-125a-5p Affects Adipocytes Proliferation, Differentiation and Fatty Acid Composition of Porcine Intramuscular Fat. (2018) Int.J.Mol.Sci. 19  ICC ;  pig.   PubMed:29414921 [IF=3.553] Xiaoyu Wang. et al. RNA-Seq and lipidomics reveal different adipogenic processes between bovine perirenal and intramuscular adipocytes. ADIPOCYTE. 2022 Aug 08  IF ;  Bovine.   PubMed:35941812 [IF=2.498] Du J et al. MicroRNA-204-5p regulates 3T3-L1 preadipocyte proliferation, apoptosis and differentiation.Gene. 2018 Aug 20;668:1-7.  ICF ;  Mouse.   PubMed:29775748 [IF=2.23] Cao, Zhongzan, et al. "Molecular cloning and expression analysis of adiponectin and its receptors (AdipoR1 and AdipoR2) in the hypothalamus of the Huoyan goose during different stages of the egg-laying cycle."Reproductive Biology and Endocrinology 13.1 (2015): 87.  WB ;   PubMed:26251033 [IF=1.922] Wang F et al. Insulin‑like growth factor I promotes adipogenesis in hemangioma stem cells from infantile hemangiomas. Mol Med Rep. 2019 Jan 24.  WB ;  Human.   PubMed:30720075 [IF=1.448] Zhang K et al. Insulin‑like growth factor 2 promotes the adipogenesis of hemangioma‑derived stem cells. Exp Ther Med. 2019 Mar;17(3):1663-1669.  WB ;  Human.   PubMed:30867686 [IF=1.399] Haodong Liu. et al. miR‐340‐5p inhibits sheep adipocyte differentiation by targeting ATF7. Anim Sci J. 2020 Jan;91(1):e13462  WB ;  Sheep.   PubMed:33190272
Research Area	immunology  Diabetes  Endocrinopathy
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	25kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Adiponectin: 151-244/244
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Adiponectin: A protein hormone produced and secreted exclusively by adipocytes (fat cells) that regulates the metabolism of lipids and glucose. Adiponectin influences the body's response to insulin. Adiponectin also has antiinflammatory effects on the cells lining the walls of blood vessels. High blood levels of adiponectin are associated with a reduced risk of heart attack. Low levels of adiponectin are found in people who are obese (and who are at increased risk of a heart attack). Adipocytes produce and secrete a number of proteins, including leptin, adipsin, properdin, and tumor necrosis factor (TNF). An adipose tissue-specific factor was isolated and termed APM1 (AdiPose Most abundant gene transcript 1). The protein product of APM1 is adiponectin. The APM1 gene maps to chromosome 3q27. Function: Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. Subunit: Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2. Interacts with CTRP9A via the C1q domain (heterotrimeric complex). Subcellular Location: Secreted. Tissue Specificity: Synthesized exclusively by adipocytes and secreted into plasma. Post-translational modifications: Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting. HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes (By similarity). O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation. DISEASE: Adiponectin deficiency (ADPND) [MIM:612556]: A condition that results in very low concentrations of plasma adiponectin. Note=The disease is caused by mutations affecting the gene represented in this entry. Diabetes mellitus, non-insulin-dependent (NIDDM) [MIM:125853]: A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry. Similarity: Contains 1 C1q domain. Contains 1 collagen-like domain. SWISS: Q15848 Gene ID: 9370 Database links: Entrez Gene: 9370 Human Entrez Gene: 11450 Mouse Entrez Gene: 246253 Rat Omim: 605441 Human SwissProt: Q15848 Human SwissProt: Q60994 Mouse Unigene: 80485 Human Unigene: 3969 Mouse 脂联素又被称作Acrp30、apM1、AdipoQ、GBP28。 最初, 脂联素是在人体皮下脂肪组织、血浆和鼠科动物的脂肪细胞中被发现人体内的脂联素由244个氨基酸组成,分子量为27KD。 脂联素影响脂肪和糖类的代谢，还可以降低多种粘附分子(VCAM-1、ICAM-1、选择素-E) 在endothelial cells的表达。并影响着Tumour坏死因子（TNF）分泌，近年来主要用于Diabetes的研究。 脂联素作为一种胰岛素超敏化激素(An Insulin–sensitizing Hormone),可以增加促进骨骼肌细胞的脂肪酸氧化和糖吸收,明显加强胰岛素的糖元异生作用,抑制肝脏的糖生成,是机体的脂质代谢和血糖稳态的调控网络中的重要调节因子，在脂肪细胞分泌的具有生物活性的一类蛋白质因子中脂联素是脂肪组织基因表达最丰富的蛋白质产物之一,大量存在于血液循环中。在人体内以3-30ug/ml的浓度出现在循环血浆中。
Product Picture	Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- Adiponectin (SL0471R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution Predicted band size: 25 kD Observed band size: 27 kD Sample: Liver (Rat) Lysate at 40 ug Intestine (Mouse) Lysate at 40 ug Primary: Anti- Adiponectin (SL0471R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution Predicted band size: 25 kD Observed band size: 25 kD Sample: Lane 1: Mouse Breast tissue lysates Lane 2: Rat Breast tissue lysates Primary: Anti-Adiponectin (SL0471R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25 kD Observed band size: 36 kD Sample: muscle (Mouse) Lysate at 40 ug Primary: Anti-Adiponectin (SL0471R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kD Tissue/cell: mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Adiponectin Polyclonal Antibody, Unconjugated(SL0471R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Adiponectin Polyclonal Antibody, Unconjugated(SL0471R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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