Product Name	Annexin V
Chinese Name	膜粘连蛋白5抗体
Alias	Anchorin CI; Annexin 5; Annexin A5; Annexin V; Annexin5; AnnexinA5; AnnexinV; ANX 5; ANX A5; ANX5; ANXA5; Calphobindin I; CBP I; CBP-I; Endonexin II; ENX 2; ENX2; Lipocortin V; PAP I; Placental anticoagulant protein I; PAP-I; Placental anticoagulant protein 4; PP 4; PP4; Thromboplastin inhibitor; VAC alpha; Vascular anticoagulant alpha; Vascular anticoagulant-alpha; ANXA5_HUMAN; Anchorin CII; Annexin-5; VAC-alpha.
literatures	Specific References  (2)     |     SL0450R has been referenced in 2 publications. [IF=15.84] Yulian Wang. et al. Delivering Antisense Oligonucleotides across the Blood‐Brain Barrier by Tumor Cell‐Derived Small Apoptotic Bodies. 2021 May 04  FC ;  Mouse.   PubMed:10.1002/advs.202004929 [IF=9.988] Jing-zhi Li. et al. RNA binding protein YTHDF1 mediates bisphenol S-induced Leydig cell damage by regulating the mitochondrial pathway of BCL2 and the expression of CDK2-CyclinE1. ENVIRON POLLUT. 2023 Jan;:121144  FCM ;  Mouse.   PubMed:36702435
Research Area	Apoptosis
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	36kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	recombind human Annexin V
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq, Jul 2008]. Function: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. Subunit: Interacts with PDCD6. Post-translational modifications: S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. DISEASE: Defects in ANXA5 are associated with susceptibility to pregnancy loss, recurrent, type 3 (RPRGL3) [MIM:614391]. A common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions. Similarity: Belongs to the annexin family. Contains 4 annexin repeats. SWISS: P08758 Gene ID: 308 Database links: Entrez Gene: 308 Human Entrez Gene: 11747 Mouse Entrez Gene: 25673 Rat Omim: 131230 Human SwissProt: P08758 Human SwissProt: P48036 Mouse SwissProt: P14668 Rat Unigene: 480653 Human Unigene: 1620 Mouse Unigene: 3318 Rat
Product Picture	Sample: Lane 1: Mouse Lung tissue lysates Lane 2: Mouse Placenta tissue lysates Lane 3: Mouse Uterus tissue lysates Lane 4: Mouse Spleen tissue lysates Lane 5: Mouse Stomach tissue lysates Lane 6: Rat Lung tissue lysates Lane 7: Rat Placenta tissue lysates Lane 8: Rat Spleen tissue lysates Lane 9: Human HepG2 cell lysates Lane 10: Human Panc-1 cell lysates Primary: Anti-Annexin V (SL0450R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD Sample: NIH/3T3(Mouse) Cell Lysate at 30 ug Lung (Mouse) Lysate at 40 ug Primary: Anti-Annexin V  (SL0450R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 35 kD Sample: PANC-1(Human) Cell Lysate at 30 ug Primary: Anti-Annexin V  (SL0450R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 35 kD Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti- Annexin V (SL0450R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37← for 20 min; Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated(SL0450R) 1:500, overnight at 4⒉C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human Neurological glioblastoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37← for 20 min; Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated(SL0450R) 1:500, overnight at 4⒉C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37← for 20 min; Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated(SL0450R) 1:500, overnight at 4⒉C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37← for 20 min; Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated(SL0450R) 1:500, overnight at 4⒉C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37← for 20 min; Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated(SL0450R) 1:500, overnight at 4⒉C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: RSC96 (blue). Primary Antibody:Rabbit Anti-Annexin V antibody(SL0450R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Antibody (SL0450R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of SL0450R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

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