Product Name	NIS
Chinese Name	钠碘转运体蛋白抗体
Alias	Na(+)/I(-) cotransporter; Na(+)/I(-) symporter; Na(+)/I(-) cotransporter; Na(+)/I(-) symporter; Na+/I- cotransporter; Na+/I-symporter; NIS; SC5A5_HUMAN; SLC5A5; sodium iodide symporter; Sodium-iodide symporter; Sodium/iodide cotransporter; Solute carrier family 5 (sodium iodide symporter) member 5; Solute carrier family 5; Solute carrier family 5 member 5.
literatures	Specific References  (11)     |     SL0448R has been referenced in 11 publications. [IF=6.291] Siying Tang. et al. GLIS3 mediated by the Rap1/PI3K/AKT signal pathway facilitates real-ambient PM2.5 exposure disturbed thyroid hormone homeostasis regulation. Ecotox Environ Safe. 2022 Mar;232:113248  WB ;  Mouse.   PubMed:35093813 [IF=5.467] Jiang B.. et al. Primary human thyrocytes maintained the function of thyroid hormone production and secretion in vitro. J ENDOCRINOL INVEST. 2023 May;:1-12  ICC ;  Human.   PubMed:37133653 [IF=4.872] Dong X et al. PM2.5 disrupts thyroid hormone homeostasis through activation of the hypothalamic-pituitary-thyroid (HPT) axis and induction of hepatic transthyretin in female rats 2.5Ecotoxicol Environ Saf.2021 Jan 15;208:111720.  IHC、WB ;  Rat.   PubMed:33396051 [IF=4.848] Shasha Hou. et al. Downregulation of miR-146b-3p Inhibits Proliferation and Migration and Modulates the Expression and Location of Sodium/Iodide Symporter in Dedifferentiated Thyroid Cancer by Potentially Targeting MUC20. Front Oncol. 2020; 10: 566365  WB,IF ;  Human.   PubMed:33489878 [IF=4.488] Niu Mengda. et al. Evaluation of [18F]tetrafluoroborate as a Potential PET Imaging Agent in a Sodium Iodide Symporter-Transfected Cell Line A549 and Endogenous NIS-Expressing Cell Lines MKN45 and K1. Mol Imaging. 2022;2022:2679260  WB ;  Human.   PubMed:10.1155/2022/2679260 [IF=4.223] Dong, Xinwen. et al. Protective effects of curcumin against thyroid hormone imbalance after gas explosion-induced traumatic brain injury via activation of the hypothalamic-pituitary-thyroid axis in male rats. ENVIRON SCI POLLUT R. 2022 May;:1-13  WB,IHC ;  Rat.   PubMed:35641736 [IF=3.565] Le Xiong. et al. Biological implications of PTEN upregulation and altered sodium/iodide symporter intracellular distribution in resveratrol-suppressed anaplastic thyroid cancer cells. J Cancer. 2020; 11(23): 6883–6891  IHC ;  Human.   PubMed:33123279 [IF=3.3] Yang H, Chen H, Guo H, Li W, Tang J, et al. (2015) Molecular Mechanisms of 2, 3′, 4, 4′, 5-Pentachlorobiphenyl-Induced Thyroid Dysfunction in FRTL-5 Cells. PLoS ONE 10(3): e0120133.  WB ;  Rat.   PubMed:25789747 [IF=3.17] Guo, Hongwei, et al. "Molecular mechanisms of human thyrocyte dysfunction induced by low concentrations of polychlorinated biphenyl 118 through the Akt/FoxO3a/NIS pathway." Journal of Applied Toxicology (2015).  WB ;  Human.   PubMed:25644787 [IF=2.19] Yao, Chen, et al. "Effect of sodium/iodide symporter (NIS)-mediated radioiodine therapy on estrogen receptor-negative breast cancer." Oncology Reports.  other ;   PubMed:25955347 [IF=1.92] Huang et al. Intracellular iodinated compounds affect sodium iodide symporter expression through TSH-mediated signaling pathways. (2011) Mol.Med.Rep. 4:77-80  WB ;  Porcine.   PubMed:21461567
Research Area	immunology  Channel protein  Transporter
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	68kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human NIS: 525-618/618 <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	catalyzes Na+/I- symporter activity plays a role in iodide transport and thyroid hormone generation. Human Sodium Iodide Symporter (hNIS) is responsible for iodide concentrating ability within thyroid follicular cells. It is a membrane bound glycoprotein with 13 membrane spanning domains and 14 extramembranous domains. It may represent an autoantigen in thyroid. Function: Mediates iodide uptake in the thyroid gland. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Expression is primarily in thyroid tissue, but also to a lower extent in mammary gland and ovary. Expression is reduced in tumors. DISEASE: Thyroid dyshormonogenesis 1 (TDH1) [MIM:274400]: A disorder characterized by the inability of the thyroid to maintain a concentration difference of readily exchangeable iodine between the plasma and the thyroid gland, leading to congenital hypothyroidism. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. SWISS: Q99PN0 Gene ID: 6528 Database links: Entrez Gene: 6528 Human Entrez Gene: 114613 Rat Omim: 601843 Human SwissProt: Q92911 Human SwissProt: Q63008 Rat Unigene: 103983 Human Unigene: 584804 Human Unigene: 10505 Rat 钠/碘转运体蛋白-NIS，是位于甲状腺细胞基底膜上的一类膜抗原，在甲状腺对碘的主动转运过程中发挥重要作用。 钠/碘Transporter受甲状腺刺激素(TSH)、碘、cell factor和生长因子等多种因素的广泛调节。自身免疫性甲状腺疾病时NIS的表达发生了明显变化，患者体内出现抗NIS自身抗体。 此外，NIS还与甲状腺冷结节和先天性甲状腺机能减退等甲状腺疾病的发病机理密切相关，近年来得到了足够的重视。
Product Picture	Sample: Lane 1: Mouse Stomach tissue lysates Lane 2: Rat Stomach tissue lysates Primary: Anti-NIS (SL0448R) at 1/200 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kDa Observed band size: 48 kDa Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NIS Polyclonal Antibody, Unconjugated(SL0448R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat thyroid gland; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NIS Polyclonal Antibody, Unconjugated(SL0448R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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