Product Name	AT2R
Chinese Name	血管紧张素Ⅱ受体1抗体
Alias	AG2S; Agtr 1; Agtr1; AGTR1_HUMAN; Agtr1a; AGTR1B; Ang II; Angiotensin II receptor type 1; Angiotensin II type 1 receptor; Angiotensin II type-1 receptor; Angiotensin receptor 1; Angiotensin receptor 1B; AT 1B; AT 1r; AT-1B; AT-1r; AT1; At1a; AT1AR; AT1B; AT1BR; AT1R; AT2R1; AT2R1A; AT2R1B; HAT1R; Type 1 angiotensin II receptor; Type 1B angiotensin II receptor; Type-1 angiotensin II receptor.
literatures	Specific References  (4)     |     SL0438R has been referenced in 4 publications. [IF=3.265] Zhu Y et al. The expressional disorder of the renal RAS mediates nephrotic syndrome of male rat offspring induced by prenatal ethanol exposure. Toxicology. 2018 May 1;400-401:9-19.  IHC-P ;  Rat.   PubMed:29548890 [IF=3.265] Li B et al. Decreased H3K9ac level of AT2R mediates the developmental origin of glomerulosclerosis induced by prenatal dexamethasone exposure in male offspring rats.(2018) Toxicology  WB ;  Rat.   PubMed:30359671 [IF=2.25] Yongtao, Zhang, et al. "Glucocorticoids activate the local renin–angiotensin system in bone: possible mechanism for glucocorticoid-induced osteoporosis."Endocrine (2014): 1-11.  ELISA ;  Rabbit.   PubMed:24519760 [IF=1.26] Jiang et al. Ginsenoside Rg3 induces ginsenoside Rb1-comparable cardioprotective effects independent of reducing blood pressure in spontaneously hypertensive rats. (2017) Exp.Ther.Med. 14:4977-4985  IHC-P ;  Rat.   PubMed:29201202
Research Area	Tumour  Cardiovascular  Cell biology  immunology  vascular endothelial cell
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	41kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human AT2R: 151-250/359 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Angiotensin II (Ang II) is an important physiological effector of blood pressure and volume regulation through vasoconstriction, aldosterone release, sodium uptake and thirst stimulation. Although Ang II interacts with two types of cell surface receptors, AT1 and AT2, most of the major cardiovascular effects seem to be mediated through AT1. Molecular cloning of the AT1 protein has shown it to be a member of the G protein-associated seven transmembrane protein receptor family. Ang II treatment of cells results in activation of several signal transduction pathways as evidenced by tyrosine phosphorylation of several proteins and induction of others. PLC?is phosphorylated after 30 seconds of treatment with Angiotensin II, indicating this as an early signal transduction event. Ang II treatment also stimulates phosphorylation of Shc, FAK and MAP kinases, and induces MKP-1, indicating stimulation of growth factor pathways. Ang II stimulation through AT1 has been shown to activate the JAK/Stat pathway involving a direct interaction between JAK2 and AT1 as demonstrated by coimmunoprecipitation. The AT1 receptor has no cytoplasmic kinase domain, but is able to function as a substrate for Src kinases and has several putative phosphorylation sites. Function: Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Subunit: Interacts with MAS1 (Probable). Interacts with ARRB1 (By similarity). Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Liver, lung, adrenal and adrenocortical adenomas. Post-translational modifications: C-terminal Ser or Thr residues may be phosphorylated. DISEASE: Defects in AGTR1 are a cause of renal tubular dysgenesis (RTD). RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P30556 Gene ID: 185 Database links: Entrez Gene: 185 Human Entrez Gene: 11607 Mouse Entrez Gene: 11608 Mouse Entrez Gene: 24180 Rat Entrez Gene: 81638 Rat Omim: 106165 Human SwissProt: P30556 Human SwissProt: P29754 Mouse SwissProt: P29755 Mouse SwissProt: P25095 Rat SwissProt: P29089 Rat Unigene: 477887 Human Unigene: 728754 Human 主要用于心、脑、血管病及Tumour的基础研究。
Product Picture	Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AT2R) Polyclonal Antibody, Unconjugated (SL0438R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AT2R/AT1 Polyclonal Antibody, Unconjugated(SL0438R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat colon tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AT2R/AT1 Polyclonal Antibody, Unconjugated(SL0438R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei Cell: (mo)Splenocytes( 2% Paraformaldehyde fixed for 10 minutes). Concentration:1:50. Incubation: 40 minutes. Host/Blank: (mo)Splenocytes. Flow cytometric analysis of Rabbit Anti-AT2R/PE-Cy5.5 Conjugated antibody (SL0438R-PE-Cy5.5)(green) compared with control in the absence of primary antibody (blue) followed by mouse spleen cells.

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