Product Name	factor VIII
Chinese Name	凝血因子8/第八凝血因子/第八因子相关抗原抗体
Alias	coagulation factor VIII; Ahf; Antihemophilic factor; Coagulation factor VIII; Coagulation factor VIII procoagulent component; Coagulation factor VIIIc; Dna segment on chromosome x unique 1253 expressed sequence; Dxs1253e; F8; F8 protein;F8c; FactorVIII; FVIII; Hema coagulation factor VIIIc procoagulent component; Hemophilia a; Hemophilia classic; OTTHUMP00000061446; Procoagulant component; FⅧ-Ag; Factor VIIIa heavy chain, 200 kDa isoform; Factor VIIIa heavy chain, 92 kDa isoform; AHF; DXS1253E; F8C; FVIII; HEMA; FA8_HUMAN.
literatures	Specific References  (11)     |     SL0434R has been referenced in 11 publications. [IF=5.59] Liu, Chengli. et al. GLP-1R Agonist Exendin-4 Protects Against Hemorrhagic Transformation Induced by rtPA After Ischemic Stroke via the Wnt/β-Catenin Signaling Pathway. Mol Neurobiol. 2022 Mar;:1-16  IF ;  Rat.   PubMed:35359227 [IF=4.967] Weijie Jiao. et al. Construction and Evaluation of Small-Diameter Bioartificial Arteries Based on a Combined-Mold Technology. POLYMERS-BASEL. 2022 Jan;14(15):3089  IF ;  Rat.   PubMed:35956602 [IF=3.935] Dongmei He. et al. Cell membrane-coated biomimetic magnetic nanoparticles for the bio-specific extraction of components from Gualou Guizhi decoction exhibiting activities against oxygen-glucose deprivation/reperfusion injury. J Pharmaceut Biomed. 2022 Feb;209:114528  ICC ;  Rat.   PubMed:34915324 [IF=2.24] Lei, Jinghui, et al. "A simple and biosafe method for isolation of human umbilical vein endothelial cells." Analytical Biochemistry 508 (2016): 15-18.  IF(ICC) ;  Human.   PubMed:27335213 [IF=2.11] Hu et al. Heparanase mediates vascular endothelial growth factor gene transcription in high-glucose human retinal microvascular endothelial cells. (2017) Mol.Vi. 23:579-587  IF(ICC) ;  Human.   PubMed:28848320 [IF=1.56] Yu, Jun-Min, et al. "Astragalosides promote angiogenesis via vascular endothelial growth factor and basic fibroblast growth factor in a rat model of myocardial infarction." Molecular Medicine Reports 12.5 (2015): 6718-6726.  IHC-P ;  Rat.   PubMed:26352430 [IF=1.5] Li, Zhe, et al. "Tanshinone IIA and Astragaloside IV promote the angiogenesis of mesenchymal stem cell-derived endothelial cell-like cells via upregulation of Cx37, Cx40 and Cx43." Experimental and Therapeutic Medicine.  Rat.   PubMed:29434774 [IF=1.28] Song, Jian, Zonghuan Li, and Aixi Yu. "Effects of preserving different veins on flow-through flap survival: An experimental study." Experimental and therapeutic medicine 11.1 (2016): 318-324.  IHC-P ;  Rabbit.   PubMed:26889261 [IF=1.26] Li et al. Tanshinone IIA and Astragaloside IV promote the angiogenesis of mesenchymal stem cell-derived endothelial cell-like cells via upregulation ofCx37,Cx40andCx43. (2018) Exp.Ther.Med. 15:1847-1854  ICC ;  Rat.   PubMed:29434774 [IF=0.58] Zhou, Yan, et al. "Changes in number and biological function of endothelial progenitor cells in hypertension disorder complicating pregnancy." Journal of Huazhong University of Science and Technology, Medical Sciences 28.6 (2008): 670.  ICF ;  Human.   PubMed:19107363 [IF=0] Ren et al. Interferon-γ and celecoxib inhibit lung-tumor growth through modulating M2/M1 macrophage ratio in the tumor microenvironment. (2014) Drug.Des.Devel.Ther. 8:1527-38  IHC ;  Mouse.   PubMed:25284985
Research Area	Tumour  immunology  Growth factors and hormones
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Rabbit, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	267kDa
Cellular localization	Extracellular matrix Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from rat factor VIII: 401-500/2258
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq, Jul 2008]. Function: Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. Subunit: Interacts with vWF. vWF binding is essential for the stabilization of F8 in circulation. Subcellular Location: Secreted, extracellular space. Post-translational modifications: Sulfation on Tyr-1699 is essential for binding vWF. DISEASE: Hemophilia A (HEMA) [MIM:306700]: A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Note=The disease is caused by mutations affecting the gene represented in this entry. Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non-functional; i.e. the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein. Similarity: Belongs to the multicopper oxidase family. Contains 3 F5/8 type A domains. Contains 2 F5/8 type C domains. Contains 6 plastocyanin-like domains. SWISS: Q7TN96 Gene ID: 2157 Database links: Entrez Gene: 403875 Dog Entrez Gene: 2157 Human Entrez Gene: 14069 Mouse Entrez Gene: 397339 Pig Omim: 300841 Human SwissProt: O18806 Dog SwissProt: P00451 Human SwissProt: Q06194 Mouse SwissProt: P12263 Pig Unigene: 654450 Human Unigene: 1805 Mouse Antigen （又称：凝结因子VIII,抗血友病因子）第VIII因子抗体用于血管源性良性和恶性Tumour的诊断，也用于遗传性血友病的研究。在正常动脉、静脉、毛细血管及心脏内细胞的vascular endothelial cell上阳性表达。第Ⅷ因子相关抗原抗体在巨核细胞及血小板上也有表达。 第Ⅷ因子相关抗原:是一种glycoprotein，广泛存在于血管上皮、肝脏、脾窦上皮、及淋巴endothelial cells，是vascular endothelial cell及其内源性良恶性Tumour的特异性标记。主要用于血管原性良恶性Tumour和血管肉瘤的诊断。少数副睾、子宫和输卵管的腺癌样瘤也有表达.
Product Picture	Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Factor VIII Polyclonal Antibody, Unconjugated(SL0434R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (factor VIII) Polyclonal Antibody, Unconjugated (SL0434R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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