Product Name	OPG
Chinese Name	骨保护蛋白/护骨素抗体
Alias	MGC29565; OCIF; OPG; Osteoclastogenesis Inhibitory Factor; TNFRSF 11B; TNFRSF11B; TR 1; TR1; Tumor Necrosis Factor Receptor Superfamily Member 11b; TR11B_HUMAN.
literatures	Specific References  (37)     |     SL0431R has been referenced in 37 publications. [IF=13.273] Xinkun Shen. et al. Improvement of aqueous stability and anti-osteoporosis properties of Zn-MOF coatings on titanium implants by hydrophobic raloxifene. Chem Eng J. 2021 Oct;:133094  WB ;  Mouse.   PubMed:10.1016/j.cej.2021.133094 [IF=8.755] Qianqian Guo. et al. Conditioned media of deer antler stem cells accelerate regeneration of alveolar bone defects in rats. CELL PROLIFERAT. 2023 Mar;:e13454  IHC ;  Deer,Rat.   PubMed:36929672 [IF=6.383] Orth M et al. Effects of locally applied adipose tissue-derived microvascular fragments by thermoresponsive hydrogel on bone healing.Acta Biomater. 2018 Sep 1;77:201-211.  WB ;  Mouse.   PubMed:30030175 [IF=6.291] Ruijun Su. et al. Rutin ameliorates the promotion effect of fine particulate matter on vascular calcification in calcifying vascular cells and ApoE-/- mice. Ecotox Environ Safe. 2022 Apr;234:113410  WB,IHC ;  Mouse,Human.   PubMed:35279519 [IF=6.289] Yutao Cui. et al. Functionalized anti-osteoporosis drug delivery system enhances osseointegration of an inorganic–organic bioactive interface in osteoporotic microenvironment. Mater Design. 2021 Aug;206:109753  IHC ;  Rabbit.   PubMed:10.1016/j.matdes.2021.109753 [IF=5.811] Lingli Ding. et al. Ginsenoside Compound K Enhances Fracture Healing via Promoting Osteogenesis and Angiogenesis.. FRONT PHARMACOL. 2022 Apr;13:855393-855393  IF ;  Rat.   PubMed:35462912 [IF=4.225] Yang Y et al. Ganoderma lucidum Immune Modulator Protein rLZ-8 Could Prevent and Reverse Bone Loss in Glucocorticoids-Induced Osteoporosis Rat Model. Front Pharmacol. 2020 May 19;11:731.  WB ;  Rat.   PubMed:32508652 [IF=4.061] Yang Man. et al. Therapeutic Potential of Liraglutide for Diabetes–Periodontitis Comorbidity: Killing Two Birds with One Stone. J DIABETES RES. 2022;2022:8260111  IHC ;  Rat.   PubMed:35845316 [IF=4] Wang, YunFei. et al. Inactivation of Ihh in Sp7-Expressing Cells Inhibits Osteoblast Proliferation, Differentiation, and Bone Formation, Resulting in a Dwarfism Phenotype with Severe Skeletal Dysplasia in Mice. CALCIFIED TISSUE INT. 2022 Jun;:1-16  IHC ;  Mouse.   PubMed:35731246 [IF=3.998] Daisuke Nishida. et al. RANKL/OPG ratio regulates odontoclastogenesis in damaged dental pulp. Sci Rep-Uk. 2021 Feb;11(1):1-12  IHC ;  Mouse.   PubMed:33633362 [IF=3.998] M M et al. Pantoprazole impairs fracture healing in aged miceSci Rep.2020 Dec 23;10(1):22376.  WB ;  Mouse.   PubMed:33361800 [IF=3.701] Shan Shan Qi. et al. Lycopene ameliorates diabetic osteoporosis via anti-inflammatory, anti-oxidation, and increasing Osteoprotegerin/RANKL expression ratio. J Funct Foods. 2021 Aug;83:104539  IHC ;  Rat.   PubMed:10.1016/j.jff.2021.104539 [IF=3.297] Hongyi Qu. et al. The effects of vasoactive intestinal peptide on RANKL-induced osteoclast formation. Ann Transl Med. 2021 Jan; 9(2): 127  WB ;  Rat.   PubMed:33569429 [IF=3.24] Hiroki Ehara. et al. Role of tissue factor in delayed bone repair induced by diabetic state in mice. Plos One. 2021 Dec;16(12):e0260754  WB ;  Mouse.   PubMed:34855855 [IF=3.08] Zhan, Fu‐Liang, Xin‐Yang Liu, and Xing‐Bo Wang. "The Role of MicroRNA‐143‐5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway." Journal of Cellular Biochemistry (2017).  WB ;  Human.   PubMed:28608628 [IF=2.99] Herath, Steven C., et al. "Stimulation of angiogenesis by cilostazol accelerates fracture healing in mice." Journal of Orthopaedic Research (2015).  WB ;  Mouse.   PubMed:26134894 [IF=2.959] Zhan et al. The Role of MicroRNA-143-5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway. (2018) J.Cell.Biochem. 119:536-546  WB ;  Human.   PubMed:28608628 [IF=2.68] Xiao, Wanan, et al. "Bone fracture healing is delayed in splenectomic rats." Life Sciences (2016).  WB ;  Rat.   PubMed:27956350 [IF=2.66] Yu, X., et al. "Denervation effectively aggravates rat experimental periodontitis." Journal of Periodontal Research (2017).  WB ;  Rat.   PubMed:28621056 [IF=2.62] Zhang, Xiaonan, et al. "Ginsenosides Rg3 attenuates glucocorticoid-induced osteoporosis through regulating BMP-2/BMPR1A/Runx2 signaling pathway."Chemico-Biological Interactions (2016).  WB ;  Rat.   PubMed:27387537 [IF=2.412] Yu X et al. CGRP gene-modified rBMSCs show better osteogenic differentiation capacity in vitro.J Mol Histol. 2018 Aug;49(4):357-367.  WB ;  Rat.   PubMed:29846865 [IF=2.31] Wei B et al. Osteoprotegerin/bone morphogenetic protein 2 combining with collagen sponges on tendon-bone healing in rabbits. J Bone Miner Metab. 2020 Jan 24.  ELISA ;  Rabbit.   PubMed:31980897 [IF=2.307] Xue Jiang. et al. Regulation of Wnt Signaling Pathway by Costic Acid Derivative, An Efficient Strategy for Treatment of Glucocorticoid-Induced Osteoporosis in Rat Model. CHEMISTRYSELECT. 2023 Apr;8(13):e202204912  WB ;  Rat.   PubMed:10.1002/slct.202204912 [IF=2.304] Teng X et al. Effects of Low Dietary Phosphorus on Tibia Quality and Metabolism in Caged Laying Hens. Prev Vet Med . 2020 Aug;181:105049.  WB ;  Hen.   PubMed:32526547 [IF=2.147] Huijuan Shen. et al. microRNA-146a mediates distraction osteogenesis via bone mesenchymal stem cell inflammatory response. ACTA HISTOCHEM. 2022 Aug;124:151913  IHC, WB ;  Dog.   PubMed:35759812 [IF=2.09] Chen, Zhiguang, et al. "Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathway."International Journal of Molecular Medicine (2016).  WB ;  Rat.   PubMed:26677102 [IF=2.082] Baixiang Wang. et al. Osteogenic effects of antihypertensive drug benidipine on mouse MC3T3-E1 cells in vitro. J Zhejiang Univ-Sc B. 2021 May;22(5):410-420  WB ;  Mouse.   PubMed:33973422 [IF=2.048] Yu X et al. Substance P aggravates periodontitis by upregulating HIF-1α and RANKL/OPG ratio. BMC Oral Health. 2019.  WB ;  Rat&Mouse.   PubMed:doi:10.21203/rs.2.16317/v1 [IF=2.048] Yan KX et al. Substance P participates in periodontitis by upregulating HIF-1α and RANKL/OPG ratio. BMC Oral Health. 2019 Dec.  WB ;  Rat.   PubMed:DOI:10.21203/rs.2.16317/v2 [IF=1.98] Yu, Xijiao, et al. "Expression of neuropeptides and bone remodeling-related factors during periodontal tissue regeneration in denervated rats." Journal of Molecular Histology: 1-9.  WB ;  Rat.   PubMed:25663522
Research Area	Tumour  Cell biology  immunology  Signal transduction  marrow cells
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	43kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human OPG: 221-300/401
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Osteoprotegerin (OPG, or osteoclastogenesis inhibitory factor) is a secretory glycoprotein belonging to TNF receptor superfamily. Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis. OPG acts as a soluble factor in the regulation of bone mass and may be beneficial in the treatment of osteoporosis with increased osteoclast activity. OPG consists of 401 amino acids with a molecular weight of 44 kDa as a monomer and 90 kDa as a disulphide-linked dimer. Function: Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis. Subunit: Homodimer. Subcellular Location: Secreted. Tissue Specificity: Highly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow. Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung. Post-translational modifications: N-glycosylated. Contains sialic acid residues. The N-terminus is blocked. DISEASE: Defects in TNFRSF11B are the cause of juvenile Paget disease (JPD) [MIM:239000]; also known as hyperostosis corticalis deformans juvenilis or hereditary hyperphosphatasia or chronic congenital idiopathic hyperphosphatasia. JPD is a rare autosomal recessive osteopathy that presents in infancy or early childhood. The disorder is characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures, and deformities due to a markedly accelerated rate of bone remodeling throughout the skeleton. Approximately 40 cases of JPD have been reported worldwide. Unless it is treated with drugs that block osteoclast-mediated skeletal resorption, the disease can be fatal. Similarity: Contains 2 death domains. Contains 4 TNFR-Cys repeats. SWISS: O08712 Gene ID: 4982 Database links: Entrez Gene: 4982 Human Omim: 602643 Human SwissProt: O00300 Human Unigene: 81791 Human 护骨素又称护骨因子(osteorotegenin,OPG)、骨保护蛋白、破骨细胞生成抑制因子、骨保护素、破骨细胞抑制生成因子（osteoclastogenesis inhibitory factor，OCIF） OPG属于TNF受体超家族，主要功能是抑制破骨细胞的分化、成熟破骨细胞的骨吸收活性，并诱导其凋亡。
Product Picture	Sample: Heart (Mouse) Lysate at 40 ug Kidney (Mouse) Lysate at 40 ug Liver (Mouse) Lysate at 40 ug Primary: Anti-OPG(SL0431R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43kD Observed band size: 50kD Sample: Placenta (Mouse) Lysate at 40 ug Liver (Mouse) Lysate at 40 ug Primary: Anti-OPG (SL 0431R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 60 kD Sample: Stomach (Mouse) Lysate at 40 ug Primary: Anti-OPG (SL 0431R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 60 kD Sample: Lane 1: Raji (Human) Cell Lysate at 30 ug Lane 2: U251 (Human) Cell Lysate at 30 ug Primary: Anti-OPG (SL0431R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD Tissue/cell: rat tibia tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Osteoprotegerin Polyclonal Antibody, Unconjugated(SL0431R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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