Product Name	GAD65
Chinese Name	谷氨酸脱羧酶-65抗体(C端）
Alias	65 kDa glutamic acid decarboxylase; DCE 2; DCE2; GAD 2; GAD 65; GAD-2; GAD-65;GAD 65, GAD2; Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa); Glutamate Decarboxylase 2; Glutamate Decarboxylase 65; Glutamate decarboxylase 65 kDa isoform; Glutamic Acid Decarboxylase 2; Glutamic Acid Decarboxylase 65.
literatures	Specific References  (1)     |     SL0400R has been referenced in 1 publications. [IF=3.57] Wen et al. Striatal and Tegmental Neurons Code Critical Signals for Temporal-Difference Learning of State Value in Domestic Chicks. (2016) Front.Neurosci. 10:476  IF(IHC-F) ;  Chicken.   PubMed:27877100
Research Area	Neurobiology  The new supersedes the old
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1ug/Test ICC=1:100  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	65kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human GAD65: 501-585/585
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008] Function: Catalyzes the production of GABA. Subunit: Homodimer. Subcellular Location: Cytoplasm, cytosol. Cytoplasmic vesicle. Cell junction, synapse, presynaptic cell membrane; Lipid-anchor. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters. Post-translational modifications: Phosphorylated; which does not affect kinetic parameters or subcellular location. Palmitoylated; which is required for presynaptic clustering. Similarity: Belongs to the group II decarboxylase family. SWISS: Q05329 Gene ID: 2572 Database links: Entrez Gene: 2572 Human Entrez Gene: 14417 Mouse Entrez Gene: 24380 Rat SwissProt: Q05329 Human SwissProt: P48320 Mouse SwissProt: Q05683 Rat Unigene: 231829 Human Unigene: 4784 Mouse Unigene: 29951 Rat 谷氨酸脱羧酶-65用于I II型Diabetes研究的很重要的蛋白。
Product Picture	Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Cerebellum tissue lysates Lane 3: Mouse Hippocampus tissue lysates Lane 4: Rat Cerebrum tissue lysates Lane 5: Rat Cerebellum tissue lysates Lane 6: Rat Hippocampus tissue lysates Lane 7: Human SH-SY5Y cell lysates Lane 8: Human U87MG cell lysates Lane 9: Human HeLa cell lysates Lane 10: Human Jurkat cell lysates Primary: Anti-GAD65 (SL0400R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kDa Observed band size: 62 kDa SHSY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GAD65) polyclonal Antibody, Unconjugated (SL0400R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Molt-4 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GAD65 Antibody(SL0400R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange). Blank control (Black line): Molt4 (Black). Primary Antibody (green line):Rabbit Anti-GAD65 antibody (SL0400R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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