Product Name	MMP9
Chinese Name	基质金属蛋白酶-9抗体
Alias	Matrix metalloproteinase-9 precursor; MMP-9; MMP9; MMP 9; 92 kDa type IV; Collagenase; 92 kDa gelatinase; Gelatinase B; GELB; MMP9_HUMAN; 82 kDa matrix metalloproteinase-9; 92 kDa type IV collagenase; CLG 4B; CLG-4B; CLG4B; Collagenase Type 4 beta; Collagenase Type-4 beta; Collagenase type IV 92 KD; Collagenase type IV 92 KD; EC 3.4.24.35; Gelatinase 92 KD; Gelatinase 92 KD; Gelatinase beta; Gelatinase-beta; GelatinaseB; GELB; Macrophage gelatinase; MANDP2; Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); Matrix Metalloproteinase 9; Type V collagenase.
literatures	Specific References  (36)     |     SL0397R has been referenced in 36 publications. [IF=17.521] Huan Lei. et al. A Combination Therapy Using Electrical Stimulation and Adaptive, Conductive Hydrogels Loaded with Self-Assembled Nanogels Incorporating Short Interfering RNA Promotes the Repair of Diabetic Chronic Wounds. Advanced Science. 2022 Sep;:2201425  IF ;  Mouse.   PubMed:36064844 [IF=8.537] Yao Y et al. Flufenamic acid inhibits secondary hemorrhage and BSCB disruption after spinal cord injury. Theranostics. 2018 Jul 30;8(15):4181-4198.   WB ;  Mouse.   PubMed:30128046 [IF=6.17] Tong Xu. et al. Lithium chloride represses abdominal aortic aneurysm via regulating GSK3β/SIRT1/NF-κB signaling pathway. Free Radical Bio Med. 2021 Apr;166:1  WB ;  Rat.   PubMed:33588051 [IF=6.155] Jialin Meng. et al. Macrophages and monocytes mediated activation of oxidative phosphorylation implicated the prognosis and clinical therapeutic strategy of Wilms tumour. COMPUT STRUCT BIOTEC. 2022 Jun;:  IHC ;  Human.   PubMed:35832632 [IF=5.777] Takahashi,et al.Novel phytopeptide osmotin mimics preventive effects of adiponectin on vascular inflammation and atherosclerosis.(2018) Metabolism: Clinical and Experimental. 83:128-138.  WB + IHC ;  Mouse.   PubMed:29410350 [IF=5.606] Luo Ping. et al. IL-37 inhibits M1-like macrophage activation to ameliorate temporomandibular joint inflammation through the NLRP3 pathway. Rheumatology. 2020 Oct;59(10):3070-3080  WB,IHC ;  Human.   PubMed:32417913 [IF=5.195] Shan Luo. et al. Application of omics technology to investigate the mechanism underlying the role of San Hua Tang in regulating microglia polarization and blood-brain barrier protection following ischemic stroke. J ETHNOPHARMACOL. 2023 May;:116640  WB ;  Rat.   PubMed:37196812 [IF=4.522] Chang J et al. miR‐363‐3p inhibits migration, invasion, and epithelial–mesenchymal transition by targeting NEDD9 and SOX4 in non‐small‐cell lung cancer. J Cell Physiol. 2019 Jul 22.  WB ;  Human.   PubMed:31332786 [IF=4.46] Xinghe Chen. et al. Cysteine and glycine rich protein 2 exacerbates vascular fibrosis in pulmonary hypertension through the nuclear translocation of yes-associated protein and transcriptional coactivator with PDZ-binding motif. TOXICOL APPL PHARM. 2022 Dec;457:116319  WB ;  Rat.   PubMed:36414118 [IF=4.162] Wenwen Chen. et al. Identification of Active Compounds and Mechanism of Huangtu Decoction for the Treatment of Ulcerative Colitis by Network Pharmacology Combined with Experimental Verification. Drug Des Dev Ther. 2021; 15: 4125–4140  IHC ;  mouse.   PubMed:34616145 [IF=3.457] Zhang J et al. Hydroxysafflor yellow A suppresses angiogenesis of hepatocellular carcinoma through inhibition of p38 MAPK phosphorylation. (2019)Biomedicine & Pharmacotherapy, 109, 806–814.  IHC ;  Mouse.   PubMed:30551534 [IF=3.405] Pallavi Shyam Kaparekar. et al. In vitro and in vivo effect of novel GA-CSNPs loaded col-fibrin nanocomposite scaffold on diabetic wound healing. J BIOMED MATER RES B. 2022 Dec;:  IHC ;  Rat.   PubMed:36583262 [IF=3.337] Hongzhong Cheng. et al. Circular RNA circLPAR3 Facilitates Esophageal Squamous Cell Carcinoma Progression Through Upregulating HMGB1 via Sponging miR-375/miR-433. Oncotargets Ther. 2020; 13: 7759–7771  WB ;  Human.   PubMed:32801782 [IF=3.269] Guansheng Liao. et al. Long non-coding RNA nuclear enriched abundant transcript 1 (NEAT1) modulates inhibitor of DNA binding 1 (ID1) to facilitate papillary thyroid carcinoma development by sponging microRNA-524-5p. BIOENGINEERED. 2022;13(5):13201-13212  WB ;  Human.   PubMed:35635748 [IF=3.23] Borin, Thaiz F., et al. "HET0016 decreases lung metastasis from breast cancer in immune-competent mouse model." PLoS One 12.6 (2017).  IHC-P ;  Mouse.   PubMed:28609459 [IF=3.216] Yanhong Wang. et al. Intermedin Alleviates Renal Ischemia-Reperfusion Injury and Enhances Neovascularization in Wistar Rats. Drug Des Dev Ther. 2020; 14: 4825–4834  IHC ;  Rat.   PubMed:33204068 [IF=3.061] Zhao Q et al. Anticancer effects of plant derived Anacardic acid on human breast cancer MDA-MB-231 cells.(2018) Am J Transl Res.Aug 15;10(8):2424-2434.  WB ;  Human.   PubMed:30210681 [IF=3.06] Jin, Meiyuan. et al. Role of ARID1A in the Regulation of Human Trophoblast Migration and Invasion. 2021 Jul 13  WB ;  Human.   PubMed:34255312 [IF=3.03] Zhao, Zhe, et al. "Inhibition of NF-kappaB activation by Pyrrolidine dithiocarbamate partially attenuates hippocampal MMP-9 activation and improves cognitive deficits in streptozotocin-induced diabetic rats."Behavioural brain research 238 (2013): 44-47.  WB ;  Rat.   PubMed:23089644 [IF=2.79] Qin, YuanHua, et al. "Recombinant human CXCL8 (3‐72) K11R/G31P regulates smooth muscle cell proliferation and migration through blockage of interleukin‐8 receptor." IUBMB life 65.1 (2013): 67-75.  Mouse.   PubMed:23281038 [IF=2.766] Li et al. Visfatin Destabilizes Atherosclerotic Plaques in Apolipoprotein E-Deficient Mice. (2016) PLoS.One. 11:e0148273  WB ;  Rat.   PubMed:26848572 [IF=2.766] Yan et al. Zinc Prevents Abdominal Aortic Aneurysm Formation by Induction of A20-Mediated Suppression of NF-κB Pathway. (2016) PLoS.One. 11:e0148536  WB ;  Rat.   PubMed:26918963 [IF=2.74] Hirokazu Konishi. et al. Effect of pemafibrate (K-877), a novel selective peroxisome proliferator-activated receptor α modular (SPPARMα), in atherosclerosis model using low density lipoprotein receptor knock-out swine with balloon injury. Plos One. 2020 Nov;15(11):e0241195  IHC ;  Pig.   PubMed:33201888 [IF=2.535] Lei J et al. LncRNA SNHG1 Alleviates IL-1β-induced Osteoarthritis by Inhibiting miR-16-5p-mediated p38 MAPK and NF-κB Signaling Pathways. Biosci Rep. 2019 Aug 5. pii: BSR20191523.  WB ;  Human.   PubMed:31383786 [IF=2.39] Basak, Sanjay, et al. "The interplay between glucose and fatty acids on tube formation and fatty acid uptake in the first trimester trophoblast cells, HTR8/SVneo." Molecular and Cellular Biochemistry (2014): 1-9.  WB ;  Human.   PubMed:25413343 [IF=2.336] Han N et al. MiR-203a-3p inhibits retinal angiogenesis and alleviates proliferative diabetic retinopathy in oxygen-induced retinopathy (OIR) rat model via targeting VEGFA and HIF-1α. Clin Exp Pharmacol Physiol. 2019 Aug 13.  WB ;  Rat&Human.   PubMed:31408201 [IF=2.247] Tort S et al. The effect of a new wound dressing on wound healing: Biochemical and histopathological evaluation. Burns. 2019 Dec 17. pii: S0305-4179(18)30976-8.  IHC-P ;  Rat.   PubMed:31862280 [IF=2.147] Gurur Garip. et al. Effect of endoplasmic reticulum stress on human trophoblast cells: Survival triggering or catastrophe resulting in death. ACTA HISTOCHEM. 2022 Oct;124:151951  IF ;  Human.   PubMed:35998395 [IF=2.014] Han S et al. The long non-coding RNA HOTTIP promotes breast cancer cell migration, invasion, and epithelial-mesenchymal transition via Wnt/β-catenin pathway. Biochem Cell Biol. 2019 Jan 24.  WB ;  Human.   PubMed:30676763 [IF=1.834] Fang Dai. et al. Effects of long term diabetogenic high fat diet on bone in ovariectomized female rats. BIOTECH HISTOCHEM. 2022 Jun 28  IHC ;  Rat.   PubMed:35762155
Research Area	Tumour  Cell biology  Neurobiology  Signal transduction  Apoptosis
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	78kDa
Cellular localization	Extracellular matrix Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human MMP9: 611-707/707
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008] Function: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Subunit: Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1. Subcellular Location: Secreted; extracellular space; extracellular matrix. Tissue Specificity: Produced by normal alveolar macrophages and granulocytes. Post-translational modifications: Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9. DISEASE: Defects in MMP9 are the cause of metaphyseal anadysplasia type 2 (MANDP2) [MIM:613073]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia. Similarity: Belongs to the peptidase M10A family. Contains 3 fibronectin type-II domains. Contains 4 hemopexin-like domains. SWISS: P14780 Gene ID: 4318 Database links: Entrez Gene: 403885 Dog Entrez Gene: 4318 Human Entrez Gene: 17395 Mouse Entrez Gene: 81687 Rat Omim: 120361 Human SwissProt: O18733 Dog SwissProt: P14780 Human SwissProt: P41245 Mouse Synthesis and Degradation（Synthesis and Degradation） MMP9亦称IV型胶原酶或明胶酶B，其主要功能为降解IV型胶原。因而它在Tumour细胞突破基底膜屏障和浸润转移中起重要作用。 目前主要用于各种恶性Tumour(如乳腺癌、胃肠道癌、卵巢癌、膀胱癌等)中的基底膜检测与转移浸润的研究。Extracellular matrix在维持正常组织结构与功能以及细胞生长和分化过程中起重要作用。Extracellular matrix动态平衡的失调与Tumour细胞侵袭、转移和复发密切相关，基质金属蛋白酶(MMP-9)是Extracellular matrix的降解酶，可降解Ⅳ、Ⅴ、Ⅸ、Ⅺ型胶原，在Tumour的浸润、转移过程中起重要作用，近年为Tumour研究的热点。 Kiss-1与MMP-9、MMP-2 是转移浸润研究的热门课题。
Product Picture	Sample: Lane 1: Human U2OS(TPA) cell lysates Primary: Anti-MMP9 (SL0397R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kDa Observed band size: 85 kDa Tissue/cell: Human laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MMP9 Polyclonal Antibody, Unconjugated(SL0397R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: Human lung cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citr Human lung cancerate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MMP9 Polyclonal Antibody, Unconjugated(SL0397R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MMP-9 Polyclonal Antibody, Unconjugated(SL0397R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat ovary carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MMP-9 Polyclonal Antibody, Unconjugated(SL0397R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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