Product Name	GLUT4
Chinese Name	葡萄糖Transporter4抗体
Alias	insulin-responsive; Glucose transporter GLUT 4; Glucose Transporter GLUT4; Glucose transporter type 4; Glucose transporter type 4 insulin responsive; GLUT 4; GLUT-4; GTR4_HUMAN; kug; SLC 2A4; SLC2A4; solute carrier family 2 (facilitated glucose transporter) member 4; Solute carrier family 2, facilitated glucose transporter member 4.
literatures	Specific References  (29)     |     SL0384R has been referenced in 29 publications. [IF=8.025] Yao Li. et al. Sodium alginate and galactooligosaccharides ameliorate metabolic disorders and alter the composition of the gut microbiota in mice with high-fat diet-induced obesity. INT J BIOL MACROMOL. 2022 Aug;215:113  WB ;  Mouse.   PubMed:35718141 [IF=6.78] Zhe Jiang. et al. RSL1D1 modulates cell senescence and proliferation via regulation of PPARγ mRNA stability. LIFE SCI. 2022 Aug;:120848  WB ;  Human.   PubMed:35940221 [IF=6.59] Yuge Jiang. et al. Eugenol improves high-fat diet/streptomycin-induced type 2 diabetes mellitus (T2DM) mice muscle dysfunction by alleviating inflammation and increasing muscle glucose uptake.. FRONT NUTR. 2022 Nov;9:1039753-1039753  WB ;  Mouse.   PubMed:36424928 [IF=6.317] Zheng Ran. et al. Microbiome–metabolomics analysis reveals the potential effect of verbascoside in alleviating cognitive impairment in db/db mice. FOOD FUNCT. 2023 Mar;:  WB ;  Mouse.   PubMed:37000613 [IF=5.988] Fu-Rong Yang. et al. Xiaoyaosan Exerts Antidepressant-Like Effect by Regulating Autophagy Involves the Expression of GLUT4 in the Mice Hypothalamic Neurons. FRONT PHARMACOL. 2022; 13: 873646  IF ;  Mouse.   PubMed:35784760 [IF=5.62] Ying-Ying Yang. et al. The human islet amyloid polypeptide reduces hippocampal tauopathy and behavioral impairments in P301S mice without inducing neurotoxicity or seeding amyloid aggregation. EXP NEUROL. 2023 Apr;362:114346  WB ;  Mouse.   PubMed:36750170 [IF=5.5] Sikder et al. High Fat Diet Upregulates Fatty Acid Oxidation and Ketogenesis via Intervention of PPAR-γ. (2018) Cell.Physiol.Biochem. 48:1317-1331  WB ;  Mouse.   PubMed:30048968 [IF=5.279] Shuhua Tian. et al. Sulforaphane Regulates Glucose and Lipid Metabolisms in Obese Mice by Restraining JNK and Activating Insulin and FGF21 Signal Pathways. J Agr Food Chem. 2021;XXXX(XXX):XXX-XXX  WB ;  Mouse.   PubMed:34706542 [IF=5.279] Shuhua Tian. et al. Sulforaphane Regulates Glucose and Lipid Metabolisms in Obese Mice by Restraining JNK and Activating Insulin and FGF21 Signal Pathways. J Agr Food Chem. 2021;XXXX(XXX):XXX-XXX  WB ;  Mouse.   PubMed:34706542 [IF=4.636] Cheng Wanpeng. et al. Transcriptomic analysis reveals the effects of maternal selenium deficiency on placental transport, hormone synthesis, and immune response in mice. METALLOMICS. 2022 Aug;:  WB ;  Mouse.   PubMed:36002020 [IF=4.362]   WB, IHC ;  Mouse.   PubMed:32973490 [IF=4.362] Zhang Yan-hui. et al. α-Lipoic Acid Maintains Brain Glucose Metabolism via BDNF/TrkB/HIF-1α Signaling Pathway in P301S Mice. Front Aging Neurosci. 2020 Aug;12:262  WB ;  Mouse.   PubMed:32973490 [IF=4.221] Huimin Wang. et al. Chronic exposure of bisphenol-A impairs cognitive function and disrupts hippocampal insulin signaling pathway in male mice. TOXICOLOGY. 2022 Apr;472:153192  WB ;  Mouse.   PubMed:35489422 [IF=4.221] Huimin Wang. et al. Chronic exposure of bisphenol-A impairs cognitive function and disrupts hippocampal insulin signaling pathway in male mice. TOXICOLOGY. 2022 Apr;472:153192  WB ;  Mouse.   PubMed:35489422 [IF=3.998] Liza D. Morales. et al. Further evidence supporting a potential role for ADH1B in obesity. Sci Rep-Uk. 2021 Jan;11(1):1-14  WB ;  Human.   PubMed:33479282 [IF=3.871] Pengli Wang. et al. The AT1 receptor autoantibody causes hypoglycemia in fetal rats via promoting the STT3A-GLUT1-glucose uptake axis in liver. Mol Cell Endocrinol. 2020 Dec;518:111022  WB,IHC ;  Rat.   PubMed:32871226 [IF=3.69] Meng-fan Peng. et al. Effects of total flavonoids from Eucommia ulmoides Oliv. leaves on polycystic ovary syndrome with insulin resistance model rats induced by letrozole combined with a high-fat diet. J Ethnopharmacol. 2021 Jun;273:113947  WB ;  Rat.   PubMed:33617969 [IF=3.3] Zhang, Shihai, et al. "Effects of isoleucine on glucose uptake through the enhancement of muscular membrane concentrations of GLUT1 and GLUT4 and intestinal membrane concentrations of Na+/glucose co-transporter 1 (SGLT-1) and GLUT2." British Journal of Nutrition (2016): 1-10.  WB ;  Mouse, Pig.   PubMed:27464458 [IF=2.659] Ye X et al. Irisin reverses insulin resistance in C2C12 cells via the p38-MAPK-PGC-1α pathway. Peptides. 2019 Jul 24:170120.  WB ;  Mouse.   PubMed:31351089 [IF=2.65] Sitong Ming. et al. Protective Effect of Shengmaiyin in Myocardial Hypertrophy-Induced Rats: A Genomic Analysis by 16S rDNA. EVID-BASED COMPL ALT. 2022 Sep 07;2022:3188292  WB ;  Rat.   PubMed:36118100 [IF=2.629] Cao Zhanhong. et al. Protective Effects of Huangqi Shengmai Yin on Type 1 Diabetes-Induced Cardiomyopathy by Improving Myocardial Lipid Metabolism. Evid-Based Compl Alt. 2021;2021:5590623  WB ;  Mouse.   PubMed:34249132 [IF=2.302] Li Q et al. All-trans retinoic acid regulates sheep primary myoblasts proliferation and differentiation in vitro. Domestic Animal Endocrinology,2019 106394.  WB ;  Sheep.   PubMed:doi:10.1016/j.domaniend.2019.106394 [IF=2.146] Xiaolan Wang. et al. The total flavonoids from Selaginella tamariscina (beauv.) Spring improve glucose and lipid metabolism in db/db mice. Iran J Basic Med Sci. 2020 Oct; 23(10): 1286–1292  WB ;  Rat.   PubMed:33149860 [IF=1.984] Pan LL et al. Urinary Metabolomics Study of the Intervention Effect of Hypoglycemic Decoction on Type 2 Diabetes Mellitus Rats Model. Evidence-Based Complementary and Alternative Medicine, 2019, 1–17.  WB ;  Rat.   PubMed:doi:10.1155/2019/1394641 [IF=1.88] Shihe Zhang. et al. Astragalus polysaccharide regulates brown adipocytes differentiation by miR-6911 targeting Prdm16. 2021 Nov 05  WB ;  Mouse.   PubMed:34738642 [IF=1.832] Zhang T et al. Dietary Sea Buckthorn Pomace Induces Beige Adipocyte Formation in Inguinal White Adipose Tissue in Lambs. Animals (Basel). 2019 Apr 24;9(4). pii: E193.  WB ;  ram lambs.   PubMed:31022943 [IF=1.58] Gao, Sujie, et al. "Propofol inhibits growth of neurons through regulating insulin receptor and insulin-like growth factor-1 receptor." Int J Clin Exp Pathol 9.7 (2016): 6785-6794.  WB ;  Rat.   PubMed:ISSN:1936-2625/IJCEP0023819 [IF=0] Afolabi Oladele Ayobami. et al. Diet-induced insulin resistance altered cardiac GLUT4 and FATP/CD36 expression in rats. Beni-Suef University Journal of Basic and Applied Sciences. 2022 Dec;11(1):1-8  IHC ;  Rat.   PubMed:10.1186/s43088-022-00312-1 [IF=0] Song B et al. The effects of emodin on insulin resistance in KKAy mice with diabetes mellitus. Phcog Mag. 2018;14:344-50.  IHC-P ;  Mouse.   PubMed:10.4103/pm.pm_362_17
Research Area	Tumour  Cardiovascular  immunology  Signal transduction  Stem cells  Diabetes
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	54kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human GLUT4: 401-509/509 <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	GLUT4 is the facilitated glucose transporter expressed exclusively in adipocytes and muscle cells, and is also known as the "insulin-responsive" glucose transporter. GLUT4 translocates from an ill-defined intracellular compartment to the plasma membrane in response to insulin. The total cellular content of GLUT4 is significantly decreased in adipose cells from many patients with Type II diabetes mellitus, and animals with some types of experimental diabetes. Function: Insulin-regulated facilitative glucose transporter. Subcellular Location: Endomembrane system. Cytoplasm > perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration. Tissue Specificity: Skeletal and cardiac muscles; brown and white fat. Post-translational modifications: Sumoylated. DISEASE: Defects in SLC2A4 may be a cause of noninsulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. Defects in SLC2A4 may be a cause of certain post-receptor defects in NIDDM. The variant in position Ile-383 is found in a small number of NIDDM patients, but seems not to be found in nondiabetic subjects. Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. SWISS: P14672 Gene ID: 6517 Database links: Entrez Gene: 282359 Cow Entrez Gene: 6517 Human Entrez Gene: 20528 Mouse Entrez Gene: 25139 Rat Omim: 138190 Human SwissProt: Q27994 Cow SwissProt: Q29RP5 Cow SwissProt: P14672 Human SwissProt: P14142 Mouse SwissProt: P19357 Rat Unigene: 380691 Human Unigene: 10661 Mouse Unigene: 1314 Rat 葡萄糖Transporter-4是一种十分重要的葡萄糖转运体,与胰岛素抵抗和2型Diabetes密切相关.葡萄糖Transporter4在细胞内部和The cell membrane之间循环流动.实现对葡萄糖的转运需要葡萄糖Transporter4自身的转位和活化.葡萄糖Transporter4与肥胖、Tumour相关联. GLUT-2和GLUT-4蛋白这两个葡萄糖运载体的研究对于Diabetes的胰岛素释放障碍和胰岛素抵抗有重要意义.
Product Picture	Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- GLUT4 (SL0384R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 54 kD Observed band size: 54 kD Sample: Heart(Rat) Cell Lysate at 40 ug Primary: Anti-GLUT4 (SL0384R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD Sample: Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 2: Heart (Mouse) Lysate at 40 ug Lane 3: Heart (Rat) Lysate at 40 ug Lane 4: Muscle (Mouse) Lysate at 40 ug Lane 5: Muscle (Rat) Lysate at 40 ug Primary: Anti-GLUT4 (SL0384R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GLUT4 Polyclonal Antibody, Unconjugated(SL0384R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GLUT4 Polyclonal Antibody, Unconjugated(SL0384R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: NIH/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GLUT4) polyclonal Antibody, Unconjugated (SL0384R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control (blue line): K562 (blue). Primary Antibody (green line): Rabbit Anti-GLUT4 antibody (SL0384R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 70% ethanol (overnight at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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