Product Name	GLUT2
Chinese Name	葡萄糖Transporter2抗体
Alias	GTR2_HUMAN; Solute carrier family 2, facilitated glucose transporter member 2; SLC2A2; Glucose transporter type 2, liver; GLUT-2; GLUT 2; solute carrier family 2 member 2;
literatures	Specific References  (14)     |     SL0351R has been referenced in 14 publications. [IF=6.576] Yu-Zhong Chen. et al. Slowly Digestible Carbohydrate Diet Ameliorates Hyperglycemia and Hyperlipidemia in High-Fat Diet/Streptozocin-Induced Diabetic Mice. FRONT NUTR. 2022; 9: 854725  IHC ;  Mouse.   PubMed:35495933 [IF=5.561] Yang Liu. et al. Methylation, Hydroxylation, Glycosylation and Acylation Affect the Transport of Wine Anthocyanins in Caco-2 Cells. FOODS. 2022 Jan;11(23):3793  WB ;  Human.   PubMed:36496602 [IF=5.23] Zheng, Liming, et al. "The Modification of Tet1 in Male Germline Stem Cells and Interact with PCNA, HDAC1 to promote their Self-renewal and Proliferation." Scientific Reports 6 (2016): 37414.  IF(IHC-P) ;  Goat.   PubMed:27857213 [IF=5.075] Qun Cheng. et al. Zearalenone Exposure Affects the Keap1–Nrf2 Signaling Patway and Glucose Nutrient Absorption Related Genes of Porcine Jejunal Epithelial Cells. TOXINS. 2022 Nov;14(11):793  WB ;  Pig.   PubMed:36422967 [IF=4.81] Justice,et al.Inhibition of acid sphingomyelinase disrupts LYNUS signaling and triggers autophagy.(2018) Journal of Lipid Research. 59:596-606.  IF(ICC) ;  Human.   PubMed:29378782 [IF=3.871] Pengli Wang. et al. The AT1 receptor autoantibody causes hypoglycemia in fetal rats via promoting the STT3A-GLUT1-glucose uptake axis in liver. Mol Cell Endocrinol. 2020 Dec;518:111022  WB,IHC ;  Rat.   PubMed:32871226 [IF=3.571] Jiang T et al. Protein-bound Anthocyanin Compounds of Purple Sweet Potato (p-BAC-PSP) Ameliorate Hyperglycemia by Regulating Hepatic Glucose Metabolism in High Fat Diet/Streptozotocin-Induced Diabetic Mice. J Agric Food Chem. 2020 Feb 12;68(6):1596-1608.  WB ;  Mouse.   PubMed:31927925 [IF=3.457] Wang N et al. Fibroblast growth factor 21 improves glucose homeostasis partially via down-regulation of Na+-d-glucose cotransporter SGLT1 in the small intestine.(2019) Biomedicine & Pharmacotherapy, 109, 1070–1077.  WB ;  Mouse.   PubMed:30551357 [IF=3.358] Guttman et al. α1-Antitrypsin modifies general NK cell interactions with dendritic cells and specific interactions with islet β-cells in favor of protection from autoimmune diabetes. (2014) Immunology.  FC/FACS ;  Mouse.   PubMed:25308894 [IF=3.144] Wang,et al.Oat globulin peptides regulate antidiabetic drug targets and glucose transporters in Caco-2 cells.(2018) Journal of Functional Foods. 42:12-20.  WB ;  Human.   PubMed:10.1016/j.jff.2017.12.061 [IF=2.984] Lihuan Zhang. et al. Effects of three probiotics and their interactions on the growth performance of and nutrient absorption in broilers. PEERJ. 2022 May;10:e13308  IHC ;  Chicken.   PubMed:35602903 [IF=2.59] Tian C et al. Short communication: A high-grain diet entails alteration in nutrient chemosensing of the rumen epithelium in goats. Animal Feed Science and Technology. Volume 262, April 2020, 114410.  IHC-P ;   PubMed:doi:10.1016/j.anifeedsci.2020.114410 [IF=1.813] Wang Zhe. et al. Total flavonoids of Astragalus Ameliorated Bile Acid Metabolism Dysfunction in Diabetes Mellitus. Evid-Based Compl Alt. 2021;2021:6675567  WB,IF ;  Human, Mouse.   PubMed:33953787 [IF=0] Song B et al. The effects of emodin on insulin resistance in KKAy mice with diabetes mellitus. Phcog Mag. 2018;14:344-50.  IHC-P ;  Mouse.   PubMed:10.4103/pm.pm_362_17
Research Area	Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat, Goat,  (predicted: Chicken, Dog, Pig, Cow, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	54kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human GLUT2: 431-524/524 <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes an integral plasma membrane glycoprotein of the liver, islet beta cells, intestine, and kidney epithelium. The encoded protein mediates facilitated bidirectional glucose transport. Because of its low affinity for glucose, it has been suggested as a glucose sensor. Mutations in this gene are associated with susceptibility to diseases, including Fanconi-Bickel syndrome and noninsulin-dependent diabetes mellitus (NIDDM). Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013] Function: Facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Liver, insulin-producing beta cell, small intestine and kidney. Post-translational modifications: N-glycosylated; required for stability and retention at the cell surface of pancreatic beta cells. DISEASE: Defects in SLC2A2 are the cause of Fanconi-Bickel syndrome (FBS) [MIM:227810]. FBS is a rare, well-defined clinical entity, inherited in an autosomal recessive mode and characterized by hepatorenal glycogen accumulation, proximal renal tubular dysfunction, and impaired utilization of glucose and galactose. Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. SWISS: P11168 Gene ID: 6514 Database links: Entrez Gene: 6514 Human Entrez Gene: 20526 Mouse Entrez Gene: 25351 Rat Omim: 138160 Human SwissProt: P11168 Human SwissProt: P14246 Mouse SwissProt: P12336 Rat Unigene: 167584 Human Unigene: 18443 Mouse Unigene: 89295 Rat
Product Picture	Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti- GLUT2 (SL0351R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54kD Observed band size: 54kD Sample: Lane 1: Kidney (Mouse) Lysate at 40 ug Lane 2: Pancreas (Mouse) Lysate at 40 ug Primary: Anti-GLUT2 (SL0351R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50-53 kD Observed band size: 50 kD Sample: HT-29 Cell (Human) Lysate at 30 ug Primary: Anti-GLUT2 (SL0351R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 59 kD Sample:Placenta (Mouse)Lysate at 40 ug Primary: Anti-GLUT2 (SL0351R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 54kD Observed band size: 54kD Sample: embyo (Mouse) Lysate at 40 ug Primary: Anti-GLUT2 (SL0351R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD Paraformaldehyde-fixed, paraffin embedded (rat pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glut2) Polyclonal Antibody, Unconjugated (SL0351R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Blank control (blue line): Hep G2(blue). Primary Antibody (green line): Rabbit Anti-GLUT2 antibody (SL0351R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 70% ethanol Overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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