Product Name	CXCR3
Chinese Name	细胞表面Chemokine受体3（CD183）抗体
Alias	CXCR3_HUMAN; Interferon-inducible protein 10 receptor; IP-10 receptor; C-X-C chemokine receptor type 3; CD 183; CD183; CD183 antigen; G protein-coupled receptor 9; G Protein Coupled Receptor 9; Chemokine (C X C motif) receptor 3; Chemokine (C X C) receptor 3; C-X-C Chemokine receptor; CXC Motif Receptor 3; Chemokine CXC Motif Receptor 3; CKRL2; CKR L2; CKR-L2; GPR9; CXC-R3; CXCR-3; IP10 receptor.
literatures	Specific References  (3)     |     SL0341R has been referenced in 3 publications. [IF=3.647] Hu Sun. et al. Study on the relationship between CXCR3 and its ligands and tubal tuberculosis. Life Sci. 2021 May;272:119047  IHC ;  Human.   PubMed:33454369 [IF=2.495] Xin Hu. et al. The role of CXCR3 and its ligands expression in Brucellar spondylitis. Bmc Immunol. 2020 Dec;21(1):1-8  IHC ;  Human.   PubMed:33208100 [IF=1.785] Xiaoqian Shang. et al. Diagnostic value of CXCR3 and its ligands in spinal tuberculosis. Exp Ther Med. 2021 Jan;21(1):1-1  IHC ;  Human.   PubMed:33365073
Research Area	Cardiovascular  Cell biology  immunology  Signal transduction  Cyclin  The cell membrane受体  G protein-coupled receptor  Cell differentiation  G protein signal
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	41kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CXCR3: 1-100/415 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4 (PMID:12782716). [provided by RefSeq, Jun 2011]. Function: Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC). Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC). Interaction with CXCL4 or CXCL10 leads to activation of the p38MAPK pathway and contributes to inhibition of angiogenesis. Overexpression in renal cancer cells down-regulates expression of the anti-apoptotic protein HMOX1 and promotes apoptosis. Isoform 3 mediates the activity of CXCL11. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta. Post-translational modifications: Sulfation on Tyr-27 and Tyr-29 is essential for CXCL10 binding and subsequent signal transduction induction. N-glycosylated. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P49682 Gene ID: 2833 Database links: Entrez Gene: 2833 Human Omim: 300574 Human SwissProt: P49682 Human Unigene: 198252 Human
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CXCR3) Polyclonal Antibody, Unconjugated (SL0341R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining. Blank control: U937 (blue). Primary Antibody:Rabbit Anti- CXCR3 antibody(SL0341R), Dilution: 1ug in 100 uL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (SL0341R, 1ug /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

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