Product Name	VEGFA
Chinese Name	血管内皮生长因子抗体
Alias	Vascuoar endothelial growth factor A; VEGF A; vascular endothelial growth factor A isoform 2 precursor; MGC70609; MVCD1; VEGF; VPF; VEGFA_HUMAN; VEGF-A; Vascular permeability factor (VPF);
literatures	Specific References  (40)     |     SL0279R has been referenced in 40 publications. [IF=16.744] Xiaoyu Ma. et al. Multifunctional injectable hydrogel promotes functional recovery after stroke by modulating microglial polarization, angiogenesis and neuroplasticity. CHEM ENG J. 2023 May;464:142520  IF ;  Mouse.   PubMed:10.1016/j.cej.2023.142520 [IF=10.383] Peiyang Gu. et al. Tailorable 3DP Flexible Scaffolds with Porosification of Filaments Facilitate Cell Ingrowth and Biomineralized Deposition. ACS APPL MATER INTER. 2022;XXXX(XXX):XXX-XXX  WB ;  Mouse.   PubMed:35829709 [IF=7.786] Shiou-Ling Lu. et al. VEGF-Mediated Augmentation of Autophagic and Lysosomal Activity in Endothelial Cells Defends against Intracellular Streptococcus pyogenes | mBio. MBIO. 2022 Jul;:  WB ;  Escherichia coli.   PubMed:35862783 [IF=6.78] Cho-Won Kim. et al. The role of KiSS1 gene on the growth and migration of prostate cancer and the underlying molecular mechanisms. LIFE SCI. 2022 Sep;:121009  IHC ;  Mouse.   PubMed:36181862 [IF=6.208] Feng Zhao. et al. Mechanism Repositioning Based on Integrative Pharmacology: Anti-Inflammatory Effect of Safflower in Myocardial Ischemia–Reperfusion Injury. INT J MOL SCI. 2023 Jan;24(6):5313  WB ;  Mouse.   PubMed:36982389 [IF=6.064] Shimaa Abdelsattar. et al. The Potential Utility of Circulating Oncofetal H19 Derived miR-675 Expression versus Tissue lncRNA-H19 Expression in Diagnosis and Prognosis of HCC in Egyptian Patients. BIOMOLECULES. 2023 Jan;13(1):3  IHC ;  Human.   PubMed:36671388 [IF=5.646] Ye T et al. MicroRNA-7 as a potential therapeutic target for aberrant NF-κB-driven distant metastasis of gastric cancer. J Exp Clin Cancer Res. 2019 Feb 6;38(1):55.  FCM&IHC ;  Mouse&Human.   PubMed:30728051 [IF=5.572] Siddharth J. Modi. et al. Discovery of VEGFR-2 inhibitors exerting significant anticancer activity against CD44+ and CD133+ cancer stem cells (CSCs): Reversal of TGF-β induced epithelial-mesenchymal transition (EMT) in hepatocellular carcinoma. Eur J Med Chem. 2020 Dec;207:112851   PubMed:33002846 [IF=5.256] Wang J et al. A Novel RNA Aptamer-modified Riboswitch as Chemical sensor. Analytica Chimica Acta. (2019).  Other ;   PubMed:doi:10.1016/j.aca.2019.11.071 [IF=5.19] Zhu, Huaisen. et al. Ginsenoside Rg1 regulates thiram-induced chondrocytes’ apoptosis and angiogenesis in broiler chickens. ENVIRON SCI POLLUT R. 2022 Dec;:1-15  WB ;  Chicken.   PubMed:36508105 [IF=4.938] Chen et al. Circulating fibrocyte mobilization in negative pressure wound therapy. (2017) J.Cell.Mol.Me. 21:1513-1522  WB ;  Rat.   PubMed:28211211 [IF=4.755] Bae Ju-Eun. et al. Effects of erythropoietin on osteoblast in the tooth extraction socket in mice periodontitis model. FRONT PHYSIOL. 2022 Oct;0:2117  IHC ;  Mouse.   PubMed:36277197 [IF=4.26] Bi et al. Peripheral serotonin regulates postoperative intra-abdominal adhesion formation in mice. (2017) Sci.Rep. 7:10001  IHC ;  Mouse.   PubMed:28855642 [IF=3.923] Yang Y et al. Inhibition of microRNA‐129‐5p expression ameliorates ultraviolet ray‐induced corneal epithelial cell injury via upregulation of EGFR. (2018) J Cell Physiol.  WB ;  Mouse.   PubMed:30515795 [IF=3.89] Ryzhov, Sergey, et al. "Role of a2b adenosine receptors in regulation of paracrine functions of stem cell antigen 1-positive cardiac stromal cells." Journal of Pharmacology and Experimental Therapeutics 341.3 (2012): 764-774.  Mouse.   PubMed:22431204 [IF=3.719] Tingting Guo. et al. Bone regeneration of mouse critical-sized calvarial defects with human mesenchymal stem cell sheets co-expressing BMP2 and VEGF. J DENT SCI. 2022 Jul;:  WB ;  Human.   PubMed:10.1016/j.jds.2022.06.020 [IF=3.571] Mei F et al. Collagen peptides isolated from Salmon salar and Tilapia nilotica skin accelerate wound healing by altering cutaneous microbiome colonization via up-regulated NOD2 and BD14. Agric. Food Chem. 2020, 68, 6, 1621-1633.  IHC-P ;  Rat.   PubMed:doi:10.1021/acs.jafc.9b08002 [IF=3.423] Qi Rao. et al. Alcohol extracts from Anemone flaccida Fr. Schmidt treat rheumatoid arthritis via inhibition of synovial hyperplasia and angiogenesis. MOL MED REP. 2023 Apr;27(4):1-12  IHC,WB ;  Rat，Human.   PubMed:36896766 [IF=3.41] Qi, Yuanyuan, et al. "Inhaled NO contributes to lung repair in piglets with acute respiratory distress syndrome via increasing circulating endothelial progenitor cells." PloS one 7.3 (2012): e33859.  WB ;  Pig.   PubMed:22448277 [IF=3.375] Zheng Chen. et al. Glycine attenuates cerebrovascular remodeling via glycine receptor alpha 2 and vascular endothelial growth factor receptor 2 after stroke. Am J Transl Res. 2020; 12(10): 6895–6907  IF,IHC ;  Rat.   PubMed:33194080 [IF=3.361] Varghese S et al. Immunostimulatory plant polysaccharides impede cancer progression and metastasis by avoiding off-target effects. Int Immunopharmacol. 2019 May 21;73:280-292.  WB ;  Human.   PubMed:31125927 [IF=3.332] Sun Y et al. The function of Piezo1 in colon cancer metastasis and its potential regulatory mechanism. J Cancer Res Clin Oncol. 2020 May;146(5):1139-1152.  IHC,WB ;  human.   PubMed:32152662 [IF=3.17] Li, Junqin, et al. "Fatty Acid Synthase Mediates the Epithelial-Mesenchymal Transition of Breast Cancer Cells." International Journal of Biological Sciences 10.2 (2014): 171-180.  WB ;  Human.   PubMed:24520215 [IF=3.14] Varghese, Sheeja, et al. "The inhibitory effect of anti-tumor polysaccharide from Punica granatum on metastasis." International Journal of Biological Macromolecules (2017).  WB ;  Human.   PubMed:28552725 [IF=2.842] Guangrui Chai. et al. NLRP3 Blockade Suppresses Pro-Inflammatory and Pro-Angiogenic Cytokine Secretion in Diabetic Retinopathy. Diabet Metab Synd Ob. 2020; 13: 3047–3058  WB ;  Human.   PubMed:32904641 [IF=2.72] Jiebin Li. et al. Targeted Temperature Management Suppresses Hypoxia-Inducible Factor-1α and Vascular Endothelial Growth Factor Expression in a Pig Model of Cardiac Arrest. 2021 Jan 05  WB ;  Pig.   PubMed:33403582 [IF=2.63] Jin Yang. et al. Protective Effect and Potential Mechanism of the Traditional Chinese Medicine Shaoyao-Gancao Decoction on Ethanol-Induced Gastric Ulcers in Rats. EVID-BASED COMPL ALT. 2022;2022:3069089  IHC ;  Rat.   PubMed:35449820 [IF=2.59] Zeng, K.-W., et al., Sprengerinin C exerts anti-tumorigenic effects in hepatocellular carcinoma via inhibition of proliferation and angiogenesis and induction of apoptosis. Eur J Pharmacol (2013)  Mouse.   PubMed:23684542 [IF=2.467] Zheng W et al. An injectable thermosensitive hydrogel for sustained release of apelin-13 to enhance flap survival in rat random skin flap. J Mater Sci Mater Med. 2019 Sep 9;30(9):106.  IHC&WB ;  Rat.   PubMed:31502009 [IF=2.219] Fernandez-Bueno I et al. Reliability and reproducibility of a rodent model of choroidal neovascularization based on the subretinal injection of polyethylene glycol.Mol Vis. 2019 Mar 18;25:194-203.  IHC-P ;  Mouse.   PubMed:30996588
Research Area	Tumour  Cardiovascular  Growth factors and hormones  Diabetes
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	24kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human VEGF: 27-120/232
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms. [provided by RefSeq]. Function: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Subunit: Homodimer; disulfide-linked. Also found as heterodimer with PGF. Subcellular Location: Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. Tissue Specificity: Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. DISEASE: Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Similarity: Belongs to the PDGF/VEGF growth factor family. SWISS: P15692 Gene ID: 7422 Database links: Entrez Gene: 7422 Human Entrez Gene: 22339 Mouse Entrez Gene: 83785 Rat Omim: 192240 Human SwissProt: P15692 Human SwissProt: Q00731 Mouse SwissProt: P16612 Rat Unigene: 73793 Human Unigene: 282184 Mouse Unigene: 1923 Rat Growth factors and hormones(Growth Factor and Hormones) vascular endothelial cell生长因子(VEGF)是一种特异作用于vascular endothelial cell的多功能cell factor,它能引起血管通透性增加,引起Extracellular matrix成分改变,诱导血管形成.在炎症、创伤愈合、心脏缺血、动脉粥样硬化、Diabetes性视网膜病变及Tumour形成等与血管生成和病变有关的诸多病理过程中起重要作用. VEGF与血管生成有关，从而也与Tumour生长也有很大关系,近年来受到很多关注.
Product Picture	Sample: Kidney(Mouse) Lysate at 30 ug Primary: Anti-VEGF (SL0279R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 25 kD Sample: Heart(Mouse) Lysate at 30 ug Primary: Anti-VEGF (SL0279R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 25 kD Sample: heart (Mouse) Lysate at 40 ug A549 Cell (Human) Lysate at 30 ug Primary: Anti- VEGF (SL0279R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 24 kD Tissue/cell: human ovary carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-VEGF Polyclonal Antibody, Unconjugated(SL0279R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Cell: Hela Concentration:1:100 Host/Isotype:Rabbit/IgG Flow cytometric analysis of Rabbit antibody (Cat#: SL0279R) on Hela(green) compared with isotype control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .

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