TEL: +86 571 56623320 EMAIL: [email protected]
Product Name AQP2 Chinese Name 水Channel protein-2抗体 Alias ADH water channel; AQP 2; AQP-2; AQP CD; AQPCD; Aquaporin 2 collecting duct; Aquaporin CD; Aquaporin2; Aquaporine 2; Collecting duct water channel protein; MGC34501; Water channel protein for renal collecting duct; WCH CD; WCHCD. literatures Specific References (1) | SL0261R has been referenced in 1 publications.[IF=2.386] Kai Zhang. et al. Investigation of Neuroprotective Effects of Erythropoietin on Chronic Neuropathic Pain in a Chronic Constriction Injury Rat Model. J Pain Res. 2020; 13: 3147–3155 WB ; Rat.Research Area immunology Signal transduction Channel protein Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 30kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human AQP2: 101-200/271 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant, and recessive forms of nephrogenic diabetes insipidus.
Function:
Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Subcellular Location:
Apical cell membrane. Cytoplasmic vesicle membrane. Shuttles from vesicles to the apical membrane.
Tissue Specificity:
Expressed in renal collecting tubules.
Post-translational modifications:
Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.
DISEASE:
Defects in AQP2 are the cause of diabetes insipidus nephrogenic autosomal (ANDI) [MIM:125800]; also known as diabetes insipidus nephrogenic type 2. ANDI is caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. It is characterized by excessive water drinking (polydypsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia. Inheritance can be autosomal dominant or recessive.
Similarity:
Belongs to the MIP/aquaporin (TC 1.A.8) family.
SWISS:
P41181
Gene ID:
359
Database links:Entrez Gene: 359 Human
Entrez Gene: 11827 Mouse
Omim: 107777 Human
SwissProt: P41181 Human
SwissProt: P56402 Mouse
Unigene: 130730 Human
Unigene: 20206 Mouse
Unigene: 90076 Rat
Channel protein(Channel Protein)
水Channel protein-2特异地存在于肾脏集合管,受血管加压素调节。有研究证明,AQP2(水Channel protein-2)在Diabetes肾脏集合管表达增强.
在1型Diabetes动物模型的水转运中尤其有意义。研究认为:AQP-2 奠定了Diabetes大鼠肾脏对水重吸收的基础,而且发现1型Diabetes病人的 AQP-2随血糖控制而减少。Product Picture Sample: Heart (Mouse) Lysate at 40 ug
Primary: Anti-Aquaporin 2 (SL0261R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 35 kD
Sample:
Kidney (Mouse) Lysate at 40 ug
293T(Human) Cell Lysate at 30 ug
Primary: Anti- Aquaporin 2 (SL0261R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 32 kD
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AQP-2 Polyclonal Antibody, Unconjugated(SL0261R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Scan Wechat Qrcode