Product Name	GAP43
Chinese Name	神经生长相关蛋白43抗体
Alias	Growth Associated Protein-43; Neuromodulin; Axonal membrane protein GAP 43; B-50; F1; GAP 43; Growth Associated Protein 43; Nerve Growth Related Peptide; Neural phosphoprotein B 50; Neuromodulin; GAP-43; pp46; NEUM_HUMAN; Protein F1; QtrA-11580; QtrA-13071.
literatures	Specific References  (7)     |     SL0154R has been referenced in 7 publications. [IF=15.84] Han Yang. et al. A Novel Targeted and High‐Efficiency Nanosystem for Combinational Therapy for Alzheimer's Disease. Adv Sci. 2020 Oct;7(19):1902906  IF,FC ;  Mouse.   PubMed:33042734 [IF=5.546] Yifan Wang. et al. Positive effect of Astragaloside IV on neurite outgrowth via talin‐dependent integrin signaling and microfilament force. J Cell Physiol. 2021 Mar;236(3):2156-2168  IF ;  Human.   PubMed:32853433 [IF=4.73] Erdal Ayhan Işik. et al. Use of Erythropoietin and Fibrin Glue Mixture for Peripheral Nerve Repair. Plast Reconstr Surg. 2022 Feb;149(2):395-403  IHC ;  Rat.   PubMed:34898529 [IF=4.411] Pingan Chen. et al. The influence of inhibiting renal neural regeneration on the efficacy of renal denervation to chronic heart failure. 2021 Oct 22  WB ;  Rat.   PubMed:34687148 [IF=3.297] Sheng Cao. et al. Ultrasound-targeted microbubble destruction-mediated Ang1 gene transfection improves left ventricular structural and sympathetic nerve remodeling in canines with myocardial infarction. Ann Transl Med. 2021 Feb; 9(3): 221  IHC ;  Dog.   PubMed:33708848 [IF=2.733] Zhai X et al. L-lactate preconditioning promotes plasticity-related proteins expression and reduces neurological deficits by potentiating GPR81 signaling in rat traumatic brain injury model. Brain Res. 2020 Nov 1;1746:146945.  IHF ;  Rat.   PubMed:32531223 [IF=1.931] Jiang et al. Effect of Frankincense Extract on Nerve Recovery in the Rat Sciatic Nerve Damage Model. (2016) Evid.Based.Complement.Alternat.Med. 2016:3617216  In Vitro/IHC ;  Rat.   PubMed:27143985
Research Area	Tumour  immunology  Neurobiology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:200-800 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	25/46kDa
Cellular localization	cytoplasmic The cell membrane Extracellular matrix
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human GAP43: 9-100/238
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Function: This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. Subunit: Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence. Subcellular Location: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Cell projection, filopodium membrane; Peripheral membrane protein. Note=Cytoplasmic surface of growth cone and synaptic plasma membranes. Post-translational modifications: Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2. Similarity: Belongs to the neuromodulin family. Contains 1 IQ domain. SWISS: P06837 Gene ID: 2596 Database links: Entrez Gene: 2596 Human Entrez Gene: 14432 Mouse Entrez Gene: 29423 Rat GenBank: NP_002036 Human Omim: 162060 Human SwissProt: P17677 Human SwissProt: P06837 Mouse SwissProt: P07936 Rat Unigene: 134974 Human Unigene: 1222 Mouse Unigene: 10928 Rat Neurobiology相关蛋白（Neurobiology）；神经Maker GAP43 （Growth associated protein-43）又称作neuromodulin，是一个轴突膜蛋白，是一种神经特异性的蛋白质，参与神经细胞外生长及突触发育形成和神经细胞再生。在神经元发育和再生过程中以高水平表达。能调解轴突延伸作用，改变细胞形态。作为细胞内信号，可大大增强与G蛋白偶联的受体转运作用。 神经生长相关蛋白-GAP-43和神经细胞黏附因子(neural cell adhesion molecule,NCAM)与突触可塑性密切相关.GAP-43是一种神经The cell membrane上的特异性磷蛋白,在神经发育和再生过程中呈现高表达,被作为突触生长的Maker,有称脊髓生长相关蛋白 GAP-43与CaM结合,参与G蛋白相互作用,神经递质的释放,作用于胞吞/胞吐过程,通过小囊溶合或诱导生长锥和突触前末端的胞吞促进膜扩展,与海马长时程增强密切相关. 神经细胞黏附因子是细胞表面glycoprotein大家族的成员之一,促进轴突生长,对长时记忆的保持有重要影响,同时,GAP-43对其具调节作用，广泛而深入地研究GAP-43意义深远。
Product Picture	Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GAP-43 Polyclonal Antibody, Unconjugated(SL0154R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAP43) Polyclonal Antibody, Unconjugated (SL0154R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-FITC) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAP43) Polyclonal Antibody, Unconjugated (SL0154R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-FITC) for 90 minutes, and DAPI for nuclei staining. Blank control (blue line): Hela cells (blue). Primary Antibody (green line): Rabbit Anti-GAP43 antibody (SL0154R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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