Product Name	Bax
Chinese Name	Bax抗体
Alias	apoptosis regulator BAX; Apoptosis regulator BAX cytoplasmic isoform beta; Apoptosis regulator BAX membrane isoform alpha; Bax isoform psi; BAX protein cytoplasmic isoform delta; Bax protein cytoplasmic isoform delta. antibody Bax protein cytoplasmic isoform gamma; Bax zeta; Bax-protein; Bcl-2-like protein 4; BCL2 associated X protein; BCL2L4; BAX_HUMAN; Bcl2-L-4.
literatures	Specific References  (186)     |     SL0127R has been referenced in 186 publications. [IF=10.75] Tian Xiangdong. et al. Autophagy Inhibition Contributes to Apoptosis of PLK4 Downregulation-induced Dormant Cells in Colorectal Cancer. INT J BIOL SCI. 2023 May;19(9):2817-2834  WB ;  Human.   PubMed:37324947 [IF=8.724] Yonggang Lv. et al. Three-dimensional decellularized tumor extracellular matrices with different stiffness as bioengineered tumor scaffolds. Bioact Mater. 2021 Sep;6:2767  WB ;  Human.   PubMed:33665508 [IF=7.419] Ziyang Huang. et al. Protective effect of ZYMT, a traditional Chinese patent medicine in a mouse model of retinitis pigmentosa. BIOMED PHARMACOTHER. 2023 Jun;162:114580  IF ;  Mouse.   PubMed:36989720 [IF=7.2] Q.T. Wei. et al. Exosome-mediated transfer of MIF confers temozolomide resistance by regulating TIMP3/PI3K/AKT axis in gliomas. Mol Ther-Oncolytics. 2021 Sep;22:114  WB ;  Human.   PubMed:34514093 [IF=6.992] Qingyue Yang. et al. The link between deacetylation and hepatotoxicity induced by exposure to hexavalent chromium. J Adv Res. 2021 Apr;:  WB ;  Rat.   PubMed:10.1016/j.jare.2021.04.002 [IF=6.854] Goreczny,et al.Hic-5 regulates fibrillar adhesion formation to control tumor extracellular matrix remodeling through interaction with tensin1.(2018) Oncogene. 37:1699-1713.  IF(ICC) ;  Human.   PubMed:29348458 [IF=6.78] Haibo Tang. et al. Tumor-targeting engineered probiotic Escherichia coli Nissle 1917 inhibits colorectal tumorigenesis and modulates gut microbiota homeostasis in mice. LIFE SCI. 2023 Jul;324:121709  IHC ;  Mouse.   PubMed:37100380 [IF=6.656] Ning Han. et al. Dihydroartemisinin elicits immunogenic death through ferroptosis-triggered ER stress and DNA damage for lung cancer immunotherapy. PHYTOMEDICINE. 2023 Jan;:154682  WB ;  Mouse.   PubMed:36739636 [IF=6.639] Amelia J. Hessheimer. et al. Somatostatin Therapy Improves Stellate Cell Activation and Early Fibrogenesis in a Preclinical Model of Extended Major Hepatectomy. Cancers. 2021 Jan;13(16):3989  IF ;  Pig.   PubMed:34439143 [IF=6.388] Weiyi Zhang. et al. Cinnamaldehyde induces apoptosis and enhances anti-colorectal cancer activity via covalent binding to HSPD1. PHYTOTHER RES. 2023 Apr;:  WB ;  Human.   PubMed:37086182 [IF=6.375] Zhou,et al.CXCR4 antagonist AMD3100 enhances the response of MDA-MB-231 triple-negative breast cancer cells to ionizing radiation.(2018) Cancer Letters. 418:196-203.  IHC-P + WB ;  Mouse.   PubMed:29317253 [IF=6.304] Zhiqiang Han. et al. PNO1 regulates autophagy and apoptosis of hepatocellular carcinoma via the MAPK signaling pathway. Cell Death Dis. 2021 May;12(6):1-14  WB ;  Human.   PubMed:34050137 [IF=6.291] Peng Zheng. et al. Alleviative effect of melatonin on the decrease of uterine receptivity caused by blood ammonia through ROS/NF-κB pathway in dairy cow. Ecotox Environ Safe. 2022 Feb;231:113166  WB ;  Bovine.   PubMed:35030520 [IF=6.291] Jia Cui. et al. Effects of ammonia on hypothalamic-pituitary-ovarian axis in female rabbits. Ecotox Environ Safe. 2021 Dec;227:112922  IHC ;  Rabbit.   PubMed:34700170 [IF=6.217] Wang L et al. Zoledronic acid inhibits the growth of cancer stem cell derived from cervical cancer cell by attenuating their stemness phenotype and inducing apoptosis and cell cycle arrest through the Erk1/2 and Akt pathways. J Exp Clin Cancer Res. 2019 Feb 21;38(1):93.  WB ;  Mouse.   PubMed:30791957 [IF=5.988] Dongfang Lv. et al. Mailuoshutong pill for varicocele-associated male infertility-Phytochemical characterisation and multitarget mechanism.. FRONT PHARMACOL. 2022 Sep;13:961011-961011  IF ;  Rat.   PubMed:36160417 [IF=5.81] Yu TT. et al. Chlorin e6-Induced Photodynamic Effect Polarizes the Macrophage Into an M1 Phenotype Through Oxidative DNA Damage and Activation of STING.. Front Pharmacol. 2022 Mar;13:837784-837784  WB ;  Mouse.   PubMed:35308251 [IF=5.714] Ting-Ting Yu. et al. Chlorin e6-induced photodynamic effect facilitates immunogenic cell death of lung cancer as a result of oxidative endoplasmic reticulum stress and DNA damage. INT IMMUNOPHARMACOL. 2023 Feb;115:109661  WB,IF ;  Mouse,Human.   PubMed:36608440 [IF=5.62] Qiu, Shi, et al. "A small peptide derived from p53 linker region can resume the apoptotic activity of p53 by sequestering iASPP with p53." Cancer Letters 356.2 (2015): 910-917.  IP ;  Human.   PubMed:25444901 [IF=5.586] Shi-Yi Xu. et al. Long-circulating doxorubicin and Schizandrin A liposome with drug-resistant liver cancer activity: in vitro and in vivo evaluation. J LIPOSOME RES. 2023 Mar 28  IHC ;  Mouse.   PubMed:36974767 [IF=5.572] Shaohua Hou. et al. Design, synthesis and biological evaluation of 1H-indazole derivatives as novel ASK1 inhibitors. Eur J Med Chem. 2021 Aug;220:113482  WB ;  Human.   PubMed:33906048 [IF=5.572] Jiayuan Luo. et al. Effects of saponins isolated from Polygonatum sibiricum on H2O2-induced oxidative damage in RIN-m5F cells and its protective effect on pancreas. FOOD CHEM TOXICOL. 2023 May;175:113724  WB,FCM ;  Rat.   PubMed:36935075 [IF=5.29] Huang, Chuen-Lin, et al. "Paraquat Induces Cell Death Through Impairing Mitochondrial Membrane Permeability." Molecular Neurobiology (2015): 1-20.  Rat.   PubMed:25947082 [IF=5.195] Fang Cao. et al. Ginkgo biloba L. extract prevents steroid-induced necrosis of the femoral head by rescuing apoptosis and dysfunction in vascular endothelial cells via the PI3K/AKT/eNOS pathway. J ETHNOPHARMACOL. 2022 Jun;:115476  WB ;  Mouse.   PubMed:35724747 [IF=5.195] Yangyang Li. et al. ASIC1a induces mitochondrial apoptotic responses in acute lung injury. EUR J PHARMACOL. 2022 Nov;934:175296  IHC, WB ;  Rat.   PubMed:36162458 [IF=5.123] Rentao Zhang. et al. Exopolysaccharide from Lactiplantibacillus plantarum YT013 and Its Apoptotic Activity on Gastric Cancer AGS Cells. Fermentation-Basel. 2023 Jun;9(6):539  WB ;  Human.   PubMed:10.3390/fermentation9060539 [IF=5.115] Yuan L et al. La2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice. Int J Nanomedicine. 2020 May 14;15:3415-3431.  IHC ;  Mouse.   PubMed:32523341 [IF=5.091] Shanyu Fang. et al. Lanthanum Chloride Sensitizes Cisplatin Resistance of Ovarian Cancer Cells via PI3K/Akt Pathway. Front Med-Lausanne. 2021; 8: 776876  WB ;  Human.   PubMed:34977076 [IF=5.051] Kangjia Du. et al. Design, synthesis, and cytotoxic activities of isaindigotone derivatives as potential anti-gastric cancer agents. J ENZYM INHIB MED CH. 2022;37(1):1212-1226  WB ;  Human.   PubMed:35450499 [IF=5.016] Min-Lin He. et al. Screening and characterization of a novel efficient tumor cell-targeting peptide derived from insulin-like growth factor binding proteins. J DRUG TARGET. 2023 Mar 28  WB ;  Human.   PubMed:36974745
Research Area	Cell biology  Signal transduction  Apoptosis  Mitochondrion
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat, Rabbit,  (predicted: Dog, Pig, Cow, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	21kDa
Cellular localization	cytoplasmic The cell membrane Mitochondrion
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Bax: 84-175/192
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]. Function: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. Subunit: Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X(L). Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Interacts with FAIM2/LFG2. Subcellular Location: Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Note=Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane. Isoform Beta: Cytoplasm. Isoform Gamma: Cytoplasm. Isoform Delta: Cytoplasm (Potential). Tissue Specificity: Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. Similarity: Belongs to the Bcl-2 family. SWISS: Q07812 Gene ID: 581 Database links: Entrez Gene: 581 Human Entrez Gene: 12028 Mouse Entrez Gene: 24887 Rat Omim: 600040 Human SwissProt: Q07812 Human SwissProt: Q07813 Mouse SwissProt: Q63690 Rat Unigene: 624291 Human Unigene: 19904 Mouse Unigene: 10668 Rat 可识别分子量为21KDa的Bax蛋白，此抗体与Bax有较高特异性，且与Bc1-2及Bc1-X蛋白无React Species，Bax、Bc1-2和Bc1-X蛋白是凋亡调节蛋白家庭成员。与Bc1-2和Bc1-X相反，Bax蛋白的过量表达加速Apoptosis。Bax在组织中广泛表达。 Bax与Bc1-2比值的高低可用于判断恶性Tumour耐药及复发。此抗体用于Tumour及Apoptosis等方面的研究。最新的研究表明：Bax可能具有Tumour抑制作用。
Product Picture	Sample: Brain(Rat)lysate 30ug; Liver(Mouse) lysates, 30ug; Primary: Anti-Bax (SL0127R) at 1:200; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 21kD Observed band size : 21kD Sample: Cerebral cortex (Rat) Lysate at 40 ug Primary: Anti-Bax (SL0127R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 21 kD Sample: Lane 1: Testis (Mouse) Lysate at 40 ug Lane 2: Kidney (Rat) Lysate at 40 ug Lane 3: Testis (Rat) Lysate at 40 ug Primary: Anti-Bax (SL0127R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 21 kD Sample: Hela-UV(Human) Cell Lysate at 30 ug Primary: Anti-Bax (SL0127R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 23 kD Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-Bax (SL0127R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 23 kD Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Polyclonal Antibody, Unconjugated (SL0127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Polyclonal Antibody, Unconjugated (SL0127R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Polyclonal Antibody, Unconjugated (SL0127R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Polyclonal Antibody, Unconjugated (SL0127R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Polyclonal Antibody, Unconjugated (SL0127R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Bax Polyclonal Antibody, Unconjugated(SL0127R) 1:200, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Bax Polyclonal Antibody, Unconjugated(SL0127R) 1:200, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Bax Polyclonal Antibody, Unconjugated(SL0127R) 1:800, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Polyclonal Antibody, Unconjugated (SL0127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Polyclonal Antibody, Unconjugated (SL0127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Polyclonal Antibody, Unconjugated (SL0127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell:Sh-sy5y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bax) polyclonal Antibody, Unconjugated (SL0127R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Tissue/cell:Sh-sy5y cell; 4% Paraformaldehyde Cartpieces 1 1Delete 5 5Delete S SDelete R RDelete + +Delete 9 9Delete Delete Delete 1 1Delete 1 1Delete $ $Delete 0 0Delete 5 5Delete $ $Delete 0 0Delete 0 0Delete 1 1Delete a aDelete a aDelete Delete i iDelete i iDelete Delete 1 1Delete Delete 1 1Delete 1 1Delete 0 0Delete Delete 2 2Delete 1 1Delete 0 0Delete 0 0Delete 0 0Delete 0 0Delete 0 0Delete 0 0Delete 1 1Delete 1 1Delete Delete - -Delete - -Delete 0 0Delete Delete t tDelete 0 0Delete 0 0Delete 0 0Delete f fDelete Delete Delete Delete Delete Delete Delete Delete Delete Delete Delete Delete Delete Delete Delete 5 5Delete $ $Delete Delete 0 0Delete Delete Delete Delete R RDelete 5 5Delete A ADelete Totalgoods,subtotals:￥Checkout Bought notes(bought amounts latest0) No one bought this product Total 0 records, divided into1 pages First Prev Next Last User Comment(Total0User Comment Num) No comment Total 0 records, divided into1 pages First Prev Next Last Username： Anonymous user E-mail： Rank： Content： Verification code： Call us +86 571 56623320 Email [email protected] Address Room 1-315, Kongle Changqing Building, No. 160 Guangye Road,Gongshu District, Hangzhou City, Zhejiang Province, China Join Us with Support Share About Us Contact Us Privacy Policy Shipping Policy Payment Policy Quality Guarantee Return & Refund policy Download Product list Leave a message Please select message type Products inquiry (price, lead time, order, etc.) Products inquiry (technical inquiry) Suggestions on website optimization Other suggestions * To protect against spam, please pass the CAPTCHA test below. +86 571 56623320 [email protected] +86 18668110335 Scan Wechat Qrcode