Product Name	Estrogen receptor beta
Chinese Name	雌激素受体β 抗体
Alias	Estrogen receptor-beta; Estrogen receptor beta; ER BETA; ER-BETA; ER-beta; Erb; ESR 2; ESR 2; ESR B; ESR-B; ESR BETA; ESR-BETA; ESR2; ESR2; ESR2_HUMAN; ESRB; ESTRB; estrogen nuclear receptor beta variant a; estrogen nuclear receptor beta variant b; estrogen receptor 2 (ER beta); Estrogen Receptor 2; Estrogen Receptor-2; estrogen receptor beta 4; NR3A2; Nuclear receptor subfamily 3 group A member 2.
literatures	Specific References  (7)     |     SL0116R has been referenced in 7 publications. [IF=5.201] Zhong Yuyi. et al. MIR143 Inhibits Steroidogenesis and Induces Apoptosis Repressed by H3K27me3 in Granulosa Cells. Front Cell Dev Biol. 2020 Oct;8:1159  WB ;  Porcine.   PubMed:33195195 [IF=4.561] Rárová,et al.The novel brassinosteroid analog BR4848 inhibits angiogenesis in human endothelial cells and induces apoptosis in human cancer cells in vitro.(2018) The Journal of Steroid Biochemistry and Molecular Biology. 178:263-271.  WB + IF(ICC) ;  Human.   PubMed:29307714 [IF=4.451] Xiaotian Xu. et al. p-Hydroxybenzoic acid alleviates inflammatory responses and intestinal mucosal damage in DSS-induced colitis by activating ERβ signaling. J Funct Foods. 2021 Dec;87:104835  WB ;  Mouse.   PubMed:10.1016/j.jff.2021.104835 [IF=4.2] Wei, Qing, et al. "Estrogen suppresses hepatocellular carcinoma cells through ERβ-mediated upregulation of the NLRP3 inflammasome." Laboratory Investigation 95.7 (2015): 804-816.  IHC-P ;  Human.   PubMed:26006022 [IF=2.561] Liu R et al. ERβ modulates genistein's cisplatin-enhancing activities in breast cancer MDA-MB-231 cells via P53-independent pathway. Mol Cell Biochem. 2019 Feb 8.  WB ;  Human.   PubMed:30737644 [IF=2.299] Gao XX et al. Effects of l-arginine on endometrial estrogen receptor α/β and progesterone receptor expression in nutrient-restricted sheep. Theriogenology. 2019 Jul 25;138:137-144.  IHC,IF&WB ;  ewe.   PubMed:31352175 [IF=1.91] Fang, Ming, Xin-Chi Wu, and Wenlong Huang. "Raloxifene Upregulated Mesangial Cell MMP-2 Activity via ER-?? Through Transcriptional Regulation."Cell Biochemistry and Biophysics (2013): 1-7..  WB ;  Mouse.   PubMed:23471663
Research Area	Tumour
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Pig, Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2μg/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	58kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ER-beta: 201-300/530
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the family of estrogenreceptors and superfamily of nuclear receptor transcriptionfactors. The gene product contains an N-terminal DNA binding domainand C-terminal ligand binding domain and is localized to thenucleus, cytoplasm, and mitochondria. Upon binding to17beta-estradiol or related ligands, the encoded protein formshomo- or hetero-dimers that interact with specific DNA sequences toactivate transcription. Some isoforms dominantly inhibit theactivity of other estrogen receptor family members. Severalalternatively spliced transcript variants of this gene have beendescribed, but the full-length nature of some of these variants hasnot been fully characterized. [provided by RefSeq, Jul 2008]. Function: Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Subunit: Interacts with SLC30A9 (By similarity). Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NCOA7 in a ligand-inducible manner. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with KDM5A. Interacts with SMARD1. Interacts with HEXIM1 and MAP1S. Interacts with PBXIP1. Interaction with MUC1 is stimulated by 7 beta-estradiol (E2) and enhances ERS1-mediated transcription. Interacts with DNTTIP2, FAM120B and UIMC1. Interacts with isoform 4 of TXNRD1. Interacts with MLL2. Interacts with ATAD2 and this interaction is enhanced by estradiol. Subcellular Location: Nucleus. Post-translational modifications: Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity. Glycosylated; contains N-acetylglucosamine, probably O-linked. Similarity: Belongs to the nuclear hormone receptor family. NR3 subfamily. Contains 1 nuclear receptor DNA-binding domain. SWISS: Q92731 Gene ID: 2100 Database links: Entrez Gene: 2100 Human Entrez Gene: 13983 Mouse Entrez Gene: 25149 Rat Omim: 601663 Human SwissProt: Q92731 Human SwissProt: O08537 Mouse SwissProt: Q62986 Rat Unigene: 660607 Human 类固醇受体（Steroid Receptors）
Product Picture	Sample: Lane 1: Testis (Mouse) Lysate at 40 ug Lane 2: Testis (Rat) Lysate at 40 ug Lane 3: Ovary (Mouse) Lysate at 40 ug Lane 4: Uterus (Mouse) Lysate at 40 ug Lane 5: Uterus (Rat) Lysate at 40 ug Primary: Anti-Estrogen receptor beta (SL0116R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55-59 kD Observed band size: 58-62 kD Sample: Kidney(Mouse) lysate at 30ug; Brain(Rat) lysate at 30ug; Primary: Anti-ER-beta (SL0116R) at 1:200; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000; Predicted band size : 58kD Observed band size : 58kD Positive control: Hela Isotype Control Antibody: Rabbit IgG ; Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 3μg in 100 μL1X PBS containing 0.5% BSA. Blank control: A431. Primary Antibody (green line): Rabbit Anti-Estrogen receptor beta antibody (SL0116R) Dilution: 2ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Cartpieces

• 
• 1

  1Delete

• 
• 5

  5Delete

• 
• S

  SDelete

• 
• R

  RDelete

• 
• +

  +Delete

• 
• 8

  8Delete

• Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 5

  5Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• E

  EDelete

• 
• E

  EDelete

• 

• Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• 
• t

  tDelete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• 
• 5

  5Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• R

  RDelete

• 
• 5

  5Delete

• 
• A

  ADelete

Totalgoods,subtotals:￥Checkout