TEL: +86 571 56623320 EMAIL: [email protected]
Product Name TGF Beta 1 Chinese Name 转化生长因子β1抗体 Alias CED; DPD1; LAP; Latency-associated peptide; TGFβ-1; TGFβ1; TGF beta; TGF-beta 1; TGF beta 1 protein; TGF-beta 1 protein; TGF-beta-1; TGF-beta-5; TGF-beta1; TGFB; Tgfb-1; tgfb1; TGFB1_HUMAN; TGFbeta; TGFbeta1; Transforming Growth Factor b1; Transforming Growth Factor beta 1; Transforming growth factor beta 1a; transforming growth factor beta-1; transforming growth factor, beta 1. TGFβ1 literatures Specific References (22) | SL0103R has been referenced in 22 publications.Research Area Cell biology Neurobiology Signal transduction Growth factors and hormones transcriptional regulatory factor cell factor The new supersedes the old Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 44kDa Detection molecular weight 48-50, 25, 12.5, 30-278, 35 kDa, 70 kDa. Cellular localization Extracellular matrix Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human TGF beta 1: 101-150/390 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease. [provided by RefSeq, Aug 2016]
Function:
Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
Subunit:
Homodimer; disulfide-linked, or heterodimer with TGFB2. Secreted and stored as a biologically inactive form in the extracellular matrix in a 290 kDa complex (large latent TGF-beta1 complex) containing the TGFB1 homodimer, the latency-associated peptide (LAP), and the latent TGFB1 binding protein-1 (LTBP1). The complex without LTBP1 is known as the'small latent TGF-beta1 complex'. Dissociation of the TGFB1 from LAP is required for growth factor activation and biological activity. Release of the large latent TGF-beta1 complex from the extracellular matrix is carried out by the matrix metalloproteinase MMP3 (By similarity). May interact with THSD4; this interaction may lead to sequestration by FBN1 microfibril assembly and attenuation of TGFB signaling. Interacts with the serine proteases, HTRA1 and HTRA3: the interaction with either inhibits TGFB1-mediated signaling. The HTRA protease activity is required for this inhibition (By similarity). Interacts with CD109, DPT and ASPN.
Subcellular Location:
Secreted, extracellular space, extracellular matrix.
Tissue Specificity:
Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
Post-translational modifications:
Glycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
DISEASE:
Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
Similarity:
Belongs to the TGF-beta family.
SWISS:
P01137
Gene ID:
7040
Database links:Entrez Gene: 7040 Human
Entrez Gene: 21803 Mouse
Omim: 190180 Human
SwissProt: P01137 Human
SwissProt: P04202 Mouse
Unigene: 645227 Human
Unigene: 248380 Mouse
Unigene: 40136 Rat
Growth factors and hormones( Growth Factor and Hormones) TGF是一种多效生长因子,对epithelial cells增值有潜在抑制作用,可抑制Tumour生长用于许多恶性Tumour如:胃癌、肺癌、膀胱癌、肾癌、前列腺癌、结肠癌等多种恶性Tumour的研究。 TGFβ超级家族由为数众多的生长的分化因子组成,包括转移生长因子β1,2和3(TGF β1,TGF β2,TGF β3);胎盘生长因子(PTGF-β);生长/分化因子(GDFs);缪氏抑制物(MIS);骨形态形成蛋白(BMPs);交织细胞元神经生长因子(GDNF);抑制素和活化素(α, β-A,和β-C),Lefty和Nodal。 TGF超级家族成员参与胚胎发育和成体组织体内平衡。TGF-β1抗体与TGF-β2和TGF-β3没有React Species。与豚鼠有大部分交叉.Product Picture Sample: K562 Cell Lysate at 40 ug
Primary: Anti- TGF Beta 1(SL0103R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 60 kD
Sample: Lymph node(Mouse) Lysate at 30 ug
Primary: Anti- TGF Beta 1(SL0103R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 54 kD
Sample: Bones(Mouse) Lysate at 30 ug
Primary: Anti- TGF Beta 1(SL0103R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 55 kD
Sample:
Lane 1: K562 (Human) Cell Lysate at 30 ug
Lane 2: U2os (Human) Cell Lysate at 30 ug
Primary:
Anti-TGF Beta 1 (SL0103R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50/35 kD
Observed band size: 50/35 kD
Tissue/cell: human Pituitary tumor; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TGF-beta1 Polyclonal Antibody, Unconjugated(SL0103R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Oral squamous cell carcinoma;4% Paraformaldehyde-fixed;
Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TGF-beta1 Polyclonal Antibody, Unconjugated(SL0103R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE-Cy3 conjugated(SL0295G-PE-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
Excitation wavelength: 530nm; Emission wavelength:578nm
Scan Wechat Qrcode