Product Name	NKB
Chinese Name	神经激肽B抗体
Alias	Neurokinin B; Gamma tachykinin 3; Neurokinin B like protein; Neurokinin B protein; Neurokinin beta; NeurokininB; Neuromedin K; NeuromedinK; NKB; NKNB; Preprotachykinin B; PreprotachykininB; PRO 1155; PRO1155; TAC 3; TAC3; Tachykinin 3; Tachykinin3; ZNEUROK 1; ZNEUROK1.
Research Area	Neurobiology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Pig, Cow, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	1.3/13kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human NKB: 81-90/121
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles. [subcellular location] secreted. It is belongs to the tachykinin family. Function: Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles. Subcellular Location: Secreted. Similarity: Belongs to the tachykinin family. SWISS: Q9UHF0 Gene ID: 6866 Database links: Entrez Gene: 281513 Cow Entrez Gene: 6866 Human Entrez Gene: 21334 Mouse Entrez Gene: 492314 Pig Entrez Gene: 29191 Rat Omim: 162330 Human SwissProt: Q9UHF0 Human SwissProt: P55099 Mouse SwissProt: P08435 Rat Unigene: 9730 Human Unigene: 2374 Mouse Unigene: 9708 Rat 此抗体识别Neurokinins A，NKA没有React Species。
Product Picture	Paraformaldehyde-fixed, paraffin embedded (MOUSE spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NKB ) Polyclonal Antibody, Unconjugated (SL0070R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NKB ) Polyclonal Antibody, Unconjugated (SL0070R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NKB ) Polyclonal Antibody, Unconjugated (SL0070R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NKB Polyclonal Antibody, Unconjugated(SL0070R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NKB Polyclonal Antibody, Unconjugated(SL0070R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei

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