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Product Name Neurokinin A Chinese Name 神经激肽A抗体 Alias Neurokinin alpha; Neurokinin K; NeurokininA; NeurokininK; Neuromedin L; NeuromedinL; Neuropeptide gamma; Neuropeptide K; NeuropeptideK; NK A; NKA; NKN A; NKNA; NPK; PPT; Preprotachykinin 1; Preprotachykinin1; Protachykinin 1 precursor; Protachykinin1 precursor; Substance K; SubstanceK; TAC 1; TAC I; TAC1; TACI; TKN 1; TKN1; neurokinin A/neuropeptide K. Research Area Neurobiology The new supersedes the old Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Chicken, Dog, Cow, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 11/13kDa Cellular localization Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human neurokinin A/neuropeptide K: 98-107/129 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes four products of the tachykinin peptide hormone family, substance P and neurokinin A, as well as the related peptides, neuropeptide K and neuropeptide gamma. These hormones are thought to function as neurotransmitters which interact with nerve receptors and smooth muscle cells. They are known to induce behavioral responses and function as vasodilators and secretagogues. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] This anitbody is crose activity with Neurokinin B.
Function:
Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.
Subcellular Location:
Secreted.
Similarity:
Belongs to the tachykinin family.
SWISS:
P20366
Gene ID:
6863
Database links:Entrez Gene: 6863 Human
Entrez Gene: 21333 Mouse
Omim: 162320 Human
SwissProt: P20366 Human
SwissProt: P41539 Mouse
Unigene: 2563 Human
Unigene: 1920 Rat
Product Picture Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Mouse Stomach tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Lane 4: Human U-2 OS cell lysates
Lane 5: Human SH-SY5Y cell lysates
Lane 6: Human u251 cell lysates
Primary: Anti-Neurokinin A (SL0069R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 11/13 kDa
Observed band size: 11 kDa
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NKA/Neurokinin A Polyclonal Antibody, Unconjugated(SL0069R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NKA/Neurokinin A Polyclonal Antibody, Unconjugated(SL0069R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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