Product Name	TGF alpha
Chinese Name	转移生长因子α抗体
Alias	EGF like TGF; ETGF; TFGA; TGF A; TGF type 1; TGFA; Transforming growth factor alpha; Transforming growth factor alpha precursor; Wa1; Waved 1; TGFA_HUMAN; TGFα; TGF-α; TGF α.
literatures	Specific References  (1)     |     SL0066R has been referenced in 1 publications. [IF=5.17] Sun et al. The correlation between microRNA490-3p and TGFα in endometrial carcinoma tumorigenesis and progression. (2016) Oncotarge. 7:9236-49  WB ;  Human.   PubMed:26843615
Research Area	Tumour  Cardiovascular  Signal transduction  Growth factors and hormones  cell factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 ELISA=1:5000-10000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	17kDa
Cellular localization	The cell membrane Extracellular matrix Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human TGF-alpha : 40-89/160
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]. Function: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar (By similarity). Inhibitor of acid secretion. Inhibitor of aminopyrine uptake in parietal cells (in vitro). Subunit: Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2. Subcellular Location: Transforming growth factor alpha: Secreted, extracellular space. Protransforming growth factor alpha: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. Similarity: Contains 1 EGF-like domain. SWISS: P01135 Gene ID: 7039 Database links: Entrez Gene: 403431 Dog Entrez Gene: 7039 Human Entrez Gene: 21802 Mouse Entrez Gene: 24827 Rat Omim: 190170 Human SwissProt: P01135 Human SwissProt: P48030 Mouse SwissProt: P01134 Rat Unigene: 170009 Human Unigene: 137222 Mouse Unigene: 9952 Rat Growth factors and hormones TGFα是一个强有力的只有丝分裂多肽,主要由恶性Tumour产生。
Product Picture	Sample: Lane 1: Mouse Kidney tissue lysates Lane 2: Mouse Colon tissue lysates Lane 3: Mouse Spleen tissue lysates Lane 4: Rat Kidney tissue lysates Lane 5: Rat Liver tissue lysates Lane 6: Rat Spleen tissue lysates Lane 7: Human Jurkat cell lysates Lane 8: Human THP-1 cell lysates Lane 9: Human A431 cell lysates Lane 10: Human HepG2 cell lysates Lane 11: Human MCF-7 cell lysates Lane 12: Human U87MG cell lysates Primary: Anti- TGF alpha (SL0066R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kDa Observed band size: 15 kDa Sample: Lane 1: Mouse Kidney tissue lysates Lane 2: Mouse Liver tissue lysates Lane 3: Rat Kidney tissue lysates Lane 4: Rat Liver tissue lysates Primary: Anti-TGF alpha (SL0066R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kDa Observed band size: 17 kDa Sample: Lane 1: Rat Kidney tissue lysates Lane 2: Rat Liver tissue lysates Primary: Anti-TGF alpha (SL0066R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kDa Observed band size: 17 kDa Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TGF alpha) Polyclonal Antibody, Unconjugated (SL0066R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TGF alpha Polyclonal Antibody, Unconjugated(SL0066R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TGF alpha Polyclonal Antibody, Unconjugated(SL0066R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control (blue line): Hela (blue). Primary Antibody (green line): Rabbit Anti-TGF alpha antibody (SL0066R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 70% methanol (Overnight at -20℃) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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