Product Name	Substance P
Chinese Name	P物质抗体
Alias	Hs.2563; Neurokinin 1; Neurokinin 2; Neurokinin A; Neurokinin alpha; Neuromedin L; Neuropeptide gamma; NKA; NKNA; PPT; Protachykinin 1 precursor; Substance K; Substance P; TAC1; TAC2; Tachykinin 1; Tachykinin 2; Tachykinin precursor 1; Tachykinin1; TACI; TKN1_HUMAN.
literatures	Specific References  (12)     |     SL0065R has been referenced in 12 publications. [IF=5.81] Shengyan Xi. et al. The Ciji-Hua’ai-Baosheng II Formula Attenuates Chemotherapy-Induced Anorexia in Mice With H22 Hepatocellular Carcinoma. Front Pharmacol. 2021; 12: 715824  IHC ;  Mouse.   PubMed:34489705 [IF=5.195] Qian Yu. et al. Yuhong ointment ameliorates inflammatory responses and wound healing in scalded mice. J ETHNOPHARMACOL. 2023 Apr;306:116118  IHC ;  Mouse.   PubMed:36642153 [IF=4.85] Daschil, Nina, et al. "L-Type calcium channel blockers and substance P induce angiogenesis of cortical vessels associated with beta-amyloid plaques in an Alzheimer mouse model." Neurobiology of Aging (2015).  IHC-F ;  Mouse.   PubMed:25619662 [IF=4.048] Jungen C et al. Increased arrhythmia susceptibility in type 2 diabetic mice related to dysregulation of ventricular sympathetic innervation. Am J Physiol Heart Circ Physiol. 2019 Oct 18.  WB ;  Mouse.   PubMed:31625779 [IF=3.411] Liu Dan. et al. RSV Promotes Epithelial Neuroendocrine Phenotype Differentiation through NODAL Signaling Pathway. Biomed Res Int. 2021;2021:9956078  IF ;  human.   PubMed:34541002 [IF=2.94] Tu, Guihua, et al. "P2X< sub> 7 inhibition in stellate ganglia prevents the increased sympathoexcitatory reflex via sensory-sympathetic coupling induced by myocardial ischemic injury." Brain research bulletin (2013).  Rat.   PubMed:23688519 [IF=2.86] Bin Feng. et al. Li-ESWT treatment reduces inflammation, oxidative stress, and pain via the PI3K/AKT/FOXO1 pathway in autoimmune prostatitis rat models. 2021 May 07  IHC ;  Rat.   PubMed:33960707 [IF=2.63] Liu, Jun, et al. "Sensory–sympathetic coupling in superior cervical ganglia after myocardial ischemic injury facilitates sympathoexcitatory action via P2X7 receptor." Purinergic Signalling (2013): 1-17.  Rat.   PubMed:23754120 [IF=1.966] Tian L et al. Gingerol inhibits cisplatin-induced acute and delayed emesis in rats and minks by regulating the central and peripheral 5-HT, SP, and DA systems. J Nat Med. 2019 Nov 25.  IHC-P ;  rat&mink.   PubMed:31768887 [IF=1.89] Yu, Xi-Jiao, et al. "Expression of substance P in rat periodontal alveolar bone after denervation." International Journal of Clinical and Experimental Pathology 9.2 (2016): 1384-1391.  IHC-P ;  Rat.   PubMed:notpostedyet [IF=0.78] Liu, Zhi-ming, et al. "Mutual Effect between Neuropeptides and Inflammatory Cytokines in Neurogenic SMSCs of Human Temporomandibular Joint." J Huazhong Univ Sci Technol [Med Sci] 34.4 (2014): 602-607.  WB ;  Human.   PubMed:25135735 [IF=0] Xiang M et al. Biyuanling suppresses the toluene-2, 4-diisocyanate induced allergic rhinitis in guinea pigs. Oncotarget. 2017 Dec 8;9(16):12620-12629.  WB&IHC-P ;  Guinea pigs.   PubMed:29560095
Research Area	Neurobiology  The new supersedes the old
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat, Guinea Pig,  (predicted: Pig, Cow, Horse, Rabbit, Sheep, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	1.4/13kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Substance P (RPKPQQFFGLM): 58-68/129
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes four products of the tachykinin peptide hormone family, substance P and neurokinin A, as well as the related peptides, neuropeptide K and neuropeptide gamma. These hormones are thought to function as neurotransmitters which interact with nerve receptors and smooth muscle cells. They are known to induce behavioral responses and function as vasodilators and secretagogues. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Function: Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles. Subcellular Location: Secreted. Similarity: Belongs to the tachykinin family. SWISS: P20366 Gene ID: 6863 Database links: Entrez Gene: 6863 Human Entrez Gene: 21333 Mouse Entrez Gene: 24806 Rat Omim: 162320 Human SwissProt: P20366 Human SwissProt: P41539 Mouse SwissProt: P06767 Rat Unigene: 2563 Human P物质（Substance P）是脑组织及消化道中发现的一种神经递质肽。由130个氨基酸残基组成，胃肠道中肌肉收缩的有力促进剂，亦为强血管扩张剂。 SP是一种脑肠肽，亦是最早发现的一种神经肽，属于速激肽家族成员，它广泛分布于中枢及外周神经系统，作为一种神经递质或调质参与疼痛、免疫、Cardiovascular等多种生理功能的调节。SP与其它的递质或调质类似，能作用于多种类型的受体，速激肽家族有NK1、NK2、NK3三种受体，都能与SP结合，其中NK1与SP的结合能力最强而被称为SP受体。
Product Picture	Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ( Substance P) Polyclonal Antibody, Unconjugated (SL0065R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. U-2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Substance P) polyclonal Antibody, Unconjugated (SL0065R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control: Mouse brain cells (blue). Primary Antibody:Rabbit Anti- Substance P antibody(SL0065R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (SL0065R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

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