Product Name	Neurokinin 1 Receptor
Chinese Name	P物质受体抗体
Alias	Neurokinin 1; Neurokinin 1 Receptor; NK 1 receptor; NK 1R; NK1 receptor; NK-1R; NK1R; NKIR; SPR; Substance P receptor; TAC 1R; TAC1R; Tachykinin 1 receptor; Tachykinin receptor 1; Tachykinin receptor 1; TACR1.  NK1-R
literatures	Specific References  (6)     |     SL0064R has been referenced in 6 publications. [IF=7.727] Jinbo Li. et al. Low dose shikonin and anthracyclines coloaded liposomes induce robust immunogenetic cell death for synergistic chemo-immunotherapy. J Control Release. 2021 Jul;335:306  IHC ;  Mouse.   PubMed:34081995 [IF=3.889] Jimei Zhang. et al. Stigmasterol alleviates allergic airway inflammation and airway hyperresponsiveness in asthma mice through inhibiting substance-P receptor. PHARM BIOL. 2023;61(1):449-458  IF ;  Human.   PubMed:36788676 [IF=3.877] Zhang et al. Effects of Neuropeptide Substance P on Proliferation and β-Cell Differentiation of Adult Pancreatic Ductal Cells. (2018) Front.Neurosci. 12:807  IHC ;  Rat.   PubMed:30455626 [IF=1.966] Tian L et al. Gingerol inhibits cisplatin-induced acute and delayed emesis in rats and minks by regulating the central and peripheral 5-HT, SP, and DA systems. J Nat Med. 2019 Nov 25.  IHC-P ;  rat&mink.   PubMed:31768887 [IF=0] 任绪莹, et al. "小半夏汤对水貂呕吐模型作用机制的研究." Acta Neuropharmacologica 1.3 (2011).  WB ;  Other Species.   PubMed:文章编号：2095-1396（2011）03-0023-004 [IF=0] 杨玉玲, et al. "姜辣素辅助昂丹司琼对顺铂所致大鼠异嗜行为的抑制作用." 世界华人消化杂志 19.10 (2011): 1034-1039.  IHC-F ;  Rat.   PubMed:doi:10.11569/wcjd.v19.i10.1034
Research Area	Neurobiology  The new supersedes the old
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat, Guinea Pig,  (predicted: Mouse, Dog, Pig, Cow, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	45kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human SPR: 2-50/407 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene belongs to a gene family of tachykinin receptors. These tachykinin receptors are characterized by interactions with G proteins and contain seven hydrophobic transmembrane regions. This gene encodes the receptor for the tachykinin substance P, also referred to as neurokinin 1. The encoded protein is also involved in the mediation of phosphatidylinositol metabolism of substance P. [provided by RefSeq, Sep 2008] Function: This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin-K. Subunit: Interacts with ARRB1 Subcellular Location: Cell membrane; Multi-pass membrane protein. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P25103 Gene ID: 6869 Database links: Entrez Gene: 6869 Human Entrez Gene: 21336 Mouse Entrez Gene: 24807 Rat Omim: 162323 Human SwissProt: P25103 Human SwissProt: P30548 Mouse SwissProt: P14600 Rat Unigene: 633301 Human Unigene: 8055 Mouse Unigene: 89609 Rat 此抗体与大、小鼠、及豚鼠均有React Species。P物质是由11个氨基酸组成的多肽，主要分布于感觉神经纤维、后根神经节和脊髓后角，作为重要的神经递质或调制因子参与伤害性刺激的传递，该递质与突触后膜上P物质受体结合，将痛冲动传入中枢，产生疼痛反应。 P物质与神经源性炎症、瘙痒及疼痛的产生有关，通过刺激肥大细胞释放组织胺、缓激肽及前列腺素等致炎、致敏、致痛物质，引起一系列病理反应，P物质除作为伤害性信息的信使外，本身又为炎症介质。
Product Picture	Sample: U251 Cell Lysate at 40 ug Primary: Anti-Neurokinin1 Receport (SL0064R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 48 kD Sample: Hela Cell Lysate at 40 ug Primary: Anti-Neurokinin1 Receport (SL0064R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 45 kD Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-SPR Polyclonal Antibody, Unconjugated(SL0064R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-SPR Polyclonal Antibody, Unconjugated(SL0064R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-SPR Polyclonal Antibody, Unconjugated(SL0064R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(SL0295G-PE)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Neurokinin 1 Receptor antibody (SL0064R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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