Product Name	Caspase-9
Chinese Name	活化半胱胺酸蛋白酶蛋白-9抗体
Alias	Caspase-9 subunit p35; Apaf-3; APAF 3; APAF3; Apoptosis related cysteine peptidase; Apoptotic protease activating factor 3; Apoptotic protease MCH 6; Apoptotic protease MCH6; CASP 9; CASP9; Caspase 9; Caspase 9 apoptosis related cysteine protease; Caspase 9 precursor; Caspase 9c; Caspase9; Caspase9 subunit p10; ICE LAP6; ICE like apoptotic protease 6; RNCASP9; MCH 6; MCH6; OTTHUMP00000044594; CASP9_HUMAN.
literatures	Specific References  (49)     |     SL0049R has been referenced in 49 publications. [IF=4.087]   WB ;  Rat.   PubMed:33300653 [IF=11.508] Qinyu Ma. et al. Osteoclast-derived apoptotic bodies couple bone resorption and formation in bone remodeling. Bone Res. 2021 Jan;9(1):1-12  WB ;  Mouse.   PubMed:33431863 [IF=7.086] Jiangnan Yi. et al. Battery wastewater induces nephrotoxicity via disordering the mitochondrial dynamics. CHEMOSPHERE. 2022 Sep;303:135018  WB ;  Mouse.   PubMed:35605732 [IF=6.551] Wei J et al. Endosulfan induces cardiotoxicity through apoptosis via unbalance of pro-survival and mitochondrial-mediated apoptotic pathways. Sci Total Environ . 2020 Jul 20;727:138790.  WB ;  human.   PubMed:32344260 [IF=6.384] Xiaowei Qin. et al. Neddylation inactivation affects cell cycle and apoptosis in sheep follicular granulosa cells. J CELL PHYSIOL. 2022 May 16  WB ;  Sheep.   PubMed:35578798 [IF=6.304] Xuemei Shen. et al. CircRILPL1 promotes muscle proliferation and differentiation via binding miR-145 to activate IGF1R/PI3K/AKT pathway. Cell Death Dis. 2021 Feb;12(2):1-14  WB ;  Bovine.   PubMed:33542215 [IF=6.291] Peng Zheng. et al. Alleviative effect of melatonin on the decrease of uterine receptivity caused by blood ammonia through ROS/NF-κB pathway in dairy cow. Ecotox Environ Safe. 2022 Feb;231:113166  WB ;  Bovine.   PubMed:35030520 [IF=6.291] Jia Cui. et al. Effects of ammonia on hypothalamic-pituitary-ovarian axis in female rabbits. Ecotox Environ Safe. 2021 Dec;227:112922  IHC ;  Rabbit.   PubMed:34700170 [IF=6.023] Shenli Zhang. et al. Prenatal EGCG exposure-induced heart mass reduction in adult male mice and underlying mechanisms. Food Chem Toxicol. 2021 Nov;157:112588  WB ;  Mouse.   PubMed:10.1016/j.fct.2021.112588 [IF=5.9] Zhao, Jing, et al. "Chronic obstructive sleep apnea causes atrial remodeling in canines: mechanisms and implications." Basic Research in Cardiology 109.5 (2014): 1-13.  WB ;  Dog.   PubMed:25015734 [IF=5.89] Rentao Zhang. et al. Anti-Gastric Cancer Activity of the Cell-free Culture Supernatant of Serofluid Dish and Lactiplantibacillus plantarum YT013. FRONT BIOENG BIOTECH. 2022; 10: 898240  WB ;  Human.   PubMed:35677304 [IF=5.75] Liu P. et al. Scalp Acupuncture Attenuates Brain Damage After Intracerebral Hemorrhage Through Enhanced Mitophagy and Reduced Apoptosis in Rats.. Front Aging Neurosci. 2021 Dec;13:718631-718631  WB ;  Rat.   PubMed:34987374 [IF=5.572] Jiayuan Luo. et al. Effects of saponins isolated from Polygonatum sibiricum on H2O2-induced oxidative damage in RIN-m5F cells and its protective effect on pancreas. FOOD CHEM TOXICOL. 2023 May;175:113724  WB,FCM ;  Rat.   PubMed:36935075 [IF=5.47] Pan, Bo, et al. "c-Abl Tyrosine Kinase Mediates Neurotoxic Prion Peptide-Induced Neuronal Apoptosis via Regulating Mitochondrial Homeostasis." Molecular Neurobiology (2014): 1-15.  Rat.   PubMed:24510275 [IF=5.201] Xuemei Shen. et al. CircINSR Regulates Fetal Bovine Muscle and Fat Development. Front Cell Dev Biol. 2020; 8: 615638  WB ;  Bovine.   PubMed:33490079 [IF=5.201] Cui Z. et al. High Expression of miR-204 in Chicken Atrophic Ovaries Promotes Granulosa Cell Apoptosis and Inhibits Autophagy.. Front Cell Dev Biol. 2020 Nov;8:580072-580072  WB ;  Chicken.   PubMed:33251211 [IF=5.123] Rentao Zhang. et al. Exopolysaccharide from Lactiplantibacillus plantarum YT013 and Its Apoptotic Activity on Gastric Cancer AGS Cells. Fermentation-Basel. 2023 Jun;9(6):539  WB ;  Human.   PubMed:10.3390/fermentation9060539 [IF=5.016] Min-Lin He. et al. Screening and characterization of a novel efficient tumor cell-targeting peptide derived from insulin-like growth factor binding proteins. J DRUG TARGET. 2023 Mar 28  WB ;  Human.   PubMed:36974745 [IF=4.927] Xiao-Jiao Chen. et al. Extracts of Knoxia roxburghii (Spreng.) M. A. Rau Induce Apoptosis in Human MCF-7 Breast Cancer Cells via Mitochondrial Pathways. MOLECULES. 2022 Jan;27(19):6435  WB ;  Human.   PubMed:36234972 [IF=4.784] Zhu B et al. The hepatoprotective effect of polysaccharides from Pleurotus ostreatus on carbon tetrachloride-induced acute liver injury rats.Int J Biol Macromol. 2019 Jun 15;131:1-9.  IHF ;  Rat.   PubMed:30851331 [IF=4.571] Zhihui Liu. et al. Curcumin alleviates aristolochic acid nephropathy based on SIRT1/Nrf2/HO-1 signaling pathway. TOXICOLOGY. 2022 Sep;479:153297  WB ;  Rat.   PubMed:10.1016/j.tox.2022.153297 [IF=4.571] Zhihui Liu. et al. Curcumin alleviates aristolochic acid nephropathy based on SIRT1/Nrf2/HO-1 signaling pathway. TOXICOLOGY. 2022 Sep;479:153297  WB ;  Rat.   PubMed:10.1016/j.tox.2022.153297 [IF=4.522] Han S et al. FHL1 regulates myoblast differentiation and autophagy through its interaction with LC3. J Cell Physiol. 2019 Oct 21.  WB ;  Chicken.   PubMed:31637727 [IF=4.087] Zhang Y et al. Gentiopicroside prevents alcoholic liver damage by improving mitochondrial dysfunction in the rat modelPhytother Res.2020 Dec 10.  IHC ;  Rat.   PubMed:33300653 [IF=3.95] Wang, Gang, et al. "Inhibition of hydrogen sulfide synthesis provides protection for severe acute pancreatitis rats via apoptosis pathway." Apoptosis (2013): 1-15.  IHC-P ;  Rat.   PubMed:23054084 [IF=3.743] Li Z et al. Zinc oxide nanoparticles induce human multiple myeloma cell death via reactive oxygen species and Cyt-C/Apaf-1/Caspase-9/Caspase-3 signaling pathway in vitro. Biomed Pharmacother. 2020 Feb;122:109712.  WB ;  Human.   PubMed:31918281 [IF=3.708] Jing Xiong. et al. Neuroprotective Effect of Sub-lethal Hyperthermia Preconditioning in a Rat Model of Repeated Closed Head Injury. NEUROSCIENCE. 2023 May;:  IF ;  Rat.   PubMed:37164305 [IF=3.687] Khalkar et al. Novel Methylselenoesters Induce Programed Cell Death via Entosis in Pancreatic Cancer Cells. (2018) Int.J.Mol.Sci. 19  WB ;   PubMed:30241340 [IF=3.367] Lijin Guo. et al. Whole Transcriptome Analysis of Chicken Bursa Reveals Candidate Gene That Enhances the Host’s Immune Response to Coccidiosis. Front Physiol. 2020; 11: 573676  WB ;  Chicken.   PubMed:33192575 [IF=3.23] Wang, Yu, et al. "Ibutilide treatment protects against ER stress induced apoptosis by regulating calumenin expression in tunicamycin treated cardiomyocytes." PloS one 12.4 (2017): e0173469.  WB ;  Rat.   PubMed:28399139
Research Area	Tumour  Cell biology  Neurobiology  Signal transduction  Apoptosis
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat, Dog,  (predicted: Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/test ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	35/50kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Caspase-9 subunit p35: 11-120/416
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Caspase 9 is inhibited by the dominant negative isoform, BclXL, cIAP1, cIAP2, XIAP, and Livin. This caspase becomes activated when recruited to Apaf1/cytochrome c complex, and following cleavage by Apaf1, granzyme B, Caspase 3, possibly Caspase 8 and Caspase 10 into large p37 and small p10 subunits. Caspase 9 intereacts with BIRC7 and has been shown to cleave PARP and vimentin. Function: Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. Subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C and ATP. Interacts (inactive form) with EFHD2. Interacts with HAX1. Interacts with BIRC2/c-IAP1, XIAP/BIRC4, BIRC5/survivin, BIRC6/bruce and BIRC7/livin. Tissue Specificity: Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes. Post-translational modifications: Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Similarity: Belongs to the peptidase C14A family. Contains 1 CARD domain. SWISS: P55211 Gene ID: 842 Database links: Entrez Gene: 842 Human Entrez Gene: 12371 Mouse Entrez Gene: 58918 Rat Omim: 602234 Human SwissProt: P55211 Human SwissProt: Q4FJK5 Mouse SwissProt: Q920G4 Rat Unigene: 329502 Human Unigene: 88829 Mouse Unigene: 32199 Rat Caspase-9半胱氨酸蛋白酶家族成员之一，又称ICE-Lap6（ICE Like apoptotease 6）参与Apoptosis过程和cell factor的加工过程，在许多胚胎和成人组织中都有分布。此抗体主要用于Tumour研究。
Product Picture	Sample: Lane 1: Mouse Stomach tissue lysates Lane 2: Mouse Spleen tissue lysates Primary: Anti-Caspase-9 (SL0049R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35/50 kDa Observed band size: 52 kDa Sample: 293T-UV Cell (Human) Lysate at 30 ug Primary: Anti-Caspase-9 (Bs- 0049R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35/50 kD Observed band size: 35/50 kD Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-9) Polyclonal Antibody, Unconjugated (SL0049R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Insulin like growth factor 1 ) Polyclonal Antibody, Unconjugated (SL0014R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Caspase-9 Polyclonal Antibody, Unconjugated(SL0049R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Caspase-9 Polyclonal Antibody, Unconjugated(SL0049R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: MCF-7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Caspase-9) Polyclonal Antibody, Unconjugated (SL0049R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-Cy3) at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Tissue/cell: HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Caspase-9) polyclonal Antibody, Unconjugated (SL0049R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Caspase-9) polyclonal Antibody, Unconjugated (SL0049R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Caspase-9) polyclonal Antibody, Unconjugated (SL0049R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control: K562 (blue). Primary Antibody:Rabbit Anti-caspase-9 antibody (SL0049R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 80% methanol (5 min) and and then permeabilized with 0.01M PBS-Tween for 20 min . Primary antibody (SL0049R, 1μg /1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (30min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min at room temperature. Acquisition of 20,000 events was performed. Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).

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