Product Name	Nestin
Chinese Name	巢蛋白/神经上皮Stem cells蛋白抗体
Alias	ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_HUMAN; Nestin.
literatures	Specific References  (2)     |     SL0006R has been referenced in 2 publications. [IF=1.405] Minyun Chen. et al. Retinoic acid induces differentiation of cochlear neural progenitor cells into hair cells. Braz J Otorhinolar. 2021 Feb;:  IF,ICC ;  Mouse.   PubMed:33707121 [IF=1.29] Fan, Lixing, et al. "Directed differentiation of aged human bone marrow multipotent stem cells effectively generates dopamine neurons." In Vitro Cellular & Developmental Biology-Animal (2013): 1-9.  Human.   PubMed:24163158
Research Area	Tumour  Cell biology  Developmental biology  Neurobiology  Stem cells  TumourCell biologyMaker
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 Flow-Cyt=1ug/Test ICC=1:100 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	178kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Nestin: 801-900/1621
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	There are a certain number of neural stem cells, which can be potentially differentiated into the neurons or neuroglia, in the adult mammalian brain. These neural precursors are located in the subependymal cell layer of the ventricle. Those precursors isolated from embryonic brain can be divided and differentiated into the neurons and glia in vitro and this is regulated by a series of growth-factor actions. Function: Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity). Subunit: Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3 (By similarity). Tissue Specificity: CNS stem cells. Post-translational modifications: Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. Similarity: Belongs to the intermediate filament family. SWISS: P48681 Gene ID: 10763 Database links: Entrez Gene: 10763 Human Entrez Gene: 18008 Mouse Entrez Gene: 25491 Rat Omim: 600915 Human SwissProt: P48681 Human SwissProt: Q6P5H2 Mouse SwissProt: P21263 Rat Unigene: 527971 Human Unigene: 331129 Mouse Unigene: 9701 Rat 结构蛋白（Structural Proteins） Neural Stem Cell Marker (神经Stem cellsMaker) 在动物体早期发育过程中，中枢神经系统发育尤为重要 。Nestin是一种中等纤维蛋白，它在哺乳动物神经前体细胞中表达较高，目前已被广泛用于神经前体细胞的重要标志分子。研究Nestin的表达调控规律，将有助于人们认识神经前体细胞的生物特性。此抗体还表达中枢神经系统Stem cells及神经epithelial cells。
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Human kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nestin) Polyclonal Antibody, Unconjugated (SL0006R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nestin) Polyclonal Antibody, Unconjugated (SL0006R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: The human brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Nestin Polyclonal Antibody, Unconjugated(SL0006R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell:U-251 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Nestin) polyclonal Antibody, Unconjugated (SL0006R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Tissue/cell:U-251 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Nestin) polyclonal Antibody, Unconjugated (SL0006R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Tissue/cell:U-251 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Nestin) polyclonal Antibody, Unconjugated (SL0006R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control (blue line): U251 (fixed with 2% paraformaldehyde (10 min)and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-Nestin antibody (SL0006R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.

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