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Product Name Anti-Transferrin receptor/CD71/RBITC Chinese Name 罗丹明(RBITC)标记的转铁蛋白受体抗体 Alias CD 71; CD71; CD71 antigen; p90; T9; TFR 1; TFR; TFR1; TFRC; TR; Transferrin receptor (p90 CD71); Transferrin Receptor Protein 1; OTTHUMP00000208523; OTTHUMP00000208524; OTTHUMP00000208525; Transferrin receptor protein 1, serum form; TRFR. Research Area Tumour Cardiovascular Cell biology immunology Neurobiology Stem cells Diabetes Cell Surface Molecule Cell type markers lymphocyte t-lymphocyte b-lymphocyte Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, ) Applications
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 74/85kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human CD71 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro.
Function:
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferring for an overlapping C-terminal binding site.
Subunit:
Homodimer; disulfide-linked. Binds one transferrin or HFE molecule per subunit. Binds the HLA class II histocompatibility antigen, DR1. Interacts with SH3BP3. Interacts with Machupo arenavirus GPC.
Subcellular Location:
Cell membrane; Single-pass type II membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Transferrin receptor protein 1, serum form: Secreted
Post-translational modifications:
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Similarity:
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.
Database links:Entrez Gene: 7037 Human
Entrez Gene: 22042 Mouse
Omim: 190010 Human
SwissProt: P02786 Human
SwissProt: Q62351 Mouse
Unigene: 529618 Human
Unigene: 28683 Mouse
Unigene: 98672 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
转铁蛋白及转铁蛋白受体(TfnrR)是机体铁代谢中的重要因子,铁是细胞生长增殖、代谢的必需元素,转铁蛋白受体(TfR)介导的胞吞作用是细胞摄取铁的主要途径。Tumour细胞因增殖生长旺盛,对铁的需求高,所以Tumour细胞有比正常细胞更多的转铁蛋白受体的表达。
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