TEL: +86 571 56623320 EMAIL: [email protected]
Product Name Anti-NF-M/PE-Cy5.5 Chinese Name PE-Cy5.5标记的中分子量神经丝蛋白抗体 Alias Neurofilament medium polypeptide; 160 kDa neurofilament protein; neurofilament 3; Nefm; 150kDa medium; NEF 3; NEF3; NEFM; Neurofilament 3; Neurofilament medium polypeptide; Neurofilament protein medium; Neurofilament triplet M protein; Neurofilament3; NF M; NF160; Neurofilament-M; Neurofilament M; NFM; NFM_HUMAN. Research Area Cell biology immunology Neurobiology Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Pig, Cow, ) Applications Flow-Cyt=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 102kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human NF-M Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
Function:
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Post-translational modifications:
There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
Similarity:
Belongs to the intermediate filament family.
Database links:Entrez Gene: 4741 Human
Entrez Gene: 18040 Mouse
Omim: 162250 Human
SwissProt: P07197 Human
SwissProt: P08553 Mouse
Unigene: 458657 Human
Unigene: 10971 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Scan Wechat Qrcode