TEL: +86 571 56623320 EMAIL: [email protected]
Product Name Anti-ATPIF1/HRP Chinese Name 辣根过氧化物酶标记的ATPIF1蛋白抗体 Alias ATIF1_HUMAN; ATP synthase inhibitor protein; ATPase inhibitor; ATPase inhibitor mitochondrial; ATPase inhibitor protein; ATPase inhibitory factor 1; ATPI; ATPIF 1; ATPIF1; ATPIP; IF(1); IF1; Inhibitor of F(1)F(o)-ATPase; IP; MGC1167; MGC8898; mitochondrial. Research Area Tumour Cell biology Signal transduction The new supersedes the old Mitochondrion Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Dog, Horse, Rabbit, ) Applications ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 10kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human ATPIF1/ATPase Inhibitory Factor 1 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes: F1, the hydrophilic catalytic core; and F0, the membrane-embedded protein channel. F1 consists of three Alpha chains and three Beta chains, which are weakly homologous, as well as one Gamma chain, one Delta chain and one Gamma chain. F0 consists of three subunits: a, b and c. A mitochondrial F1-ATPase inhibitor protein, ATPIF1 (ATPase inhibitory factor 1), also known as IP, IF1, ATPI or ATPIP (ATPase inhibitor protein), binds to the C-terminal region of a Beta subunit of the F1-ATPase at low pH values and, via interference of the Beta and Gamma subunit interaction, ATPIF1 regulates the activity of the F1F0-ATPase. This reversible ATPIF1 binding to F1F0-ATPase also occurs on the surface of endothelial cells.
Function:
Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix.
Subunit:
Homodimer; represents the active form and is present at a pH value below 6.5. Homotetramer; represents the inactive form and is present at a pH value above 7.0 (By similarity).
Subcellular Location:
Mitochondrion.
Post-translational modifications:
Exhibits variability in chain length, mitochondria have distinct pools of protein cleaved after the 24th, 25th, and 26th amino acid.
Similarity:
Belongs to the ATPase inhibitor family.
Database links:Entrez Gene: 93974 Human
Entrez Gene: 11983 Mouse
SwissProt: Q9UII2 Human
SwissProt: O35143 Mouse
Unigene: 59150 Cow
Unigene: 590908 Human
Unigene: 726089 Human
Unigene: 2171 Mouse
Unigene: 1658 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Scan Wechat Qrcode