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Product Name Anti-PABPC4/Biotin Chinese Name 生物素标记的多聚腺苷酸Binding protein4抗体 Alias Activated platelet protein 1; Activated-platelet protein 1; APP 1; APP-1; APP1; FLJ43938; Inducible poly(A) binding protein; Inducible poly(A)-binding protein; IPABP; IPABP4; PABP 4; PABP-4; PABP4; PABP4_HUMAN; PABPC 4; PABPC4; Poly A binding protein cytoplasmic 4; Poly(A) binding protein 4; Poly(A) binding protein cytoplasmic 4 (inducible form); Poly(A) binding protein cytoplasmic 4; Poly(A)-binding protein 4; Polyadenylate binding protein 4; Polyadenylate-binding protein 4; Polyadenylate-binding protein, cytoplasmic, 4; Polyadenylate-binding protein, inducible. Research Area Cell biology Binding protein Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) Applications WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 71kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human PABPC4 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
Function:
Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo.
Subunit:
Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with NFX1.
Subcellular Location:
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Tissue Specificity:
Expressed at low levels in resting normal T cells; following T-cell activation, however, mRNA levels are rapidly up-regulated.
Post-translational modifications:
Arg-518 is dimethylated, probably to asymmetric dimethylarginine.
Similarity:
Belongs to the polyadenylate-binding protein type-1 family.
Contains 1 PABC domain.
Contains 4 RRM (RNA recognition motif) domains.
Database links:Entrez Gene: 8761 Human
Entrez Gene: 230721 Mouse
Omim: 603407 Human
SwissProt: Q13310 Human
Unigene: 169900 Human
Unigene: 277091 Mouse
Unigene: 201330 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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