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Product Name Anti-MED30/Biotin Chinese Name 生物素标记的MED30蛋白抗体 Alias TRAP25; MED30; MED30_HUMAN; Mediator complex subunit 30; Mediator of RNA polymerase II transcription subunit 30; THRAP6; Thyroid hormone receptor-associated protein 6; Thyroid hormone receptor-associated protein complex 25 kDa component; TRAP/Mediator complex component TRAP25; Trap25. Research Area Cell biology transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Dog, Pig, Horse, Rabbit, Sheep, ) Applications WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 20kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human MED30 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
The multiprotein TRAP/Mediator complex facilitates gene expression through a wide variety of transcriptional activators. MED30 is a component of this complex that appears to be metazoan specific (Baek et al., 2002 [PubMed 11909976]).[supplied by OMIM, Nov 2010]
Function:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Subcellular Location:
Nucleus.
Tissue Specificity:
Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
Similarity:
Belongs to the Mediator complex subunit 30 family.
Database links:Entrez Gene: 90390 Human
Entrez Gene: 69790 Mouse
Omim: 610237 Human
SwissProt: Q96HR3 Human
SwissProt: Q9CQI9 Mouse
Unigene: 492612 Human
Unigene: 195562 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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