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Product Name Anti-EPX/Biotin Chinese Name 生物素标记的嗜酸性粒细胞过氧化物酶抗体 Alias Eosinophil peroxidase; Eosinophil peroxidase heavy chain; EPER; EPO; EPP; EPX PEN; PERE; PERE_HUMAN. Research Area Tumour immunology Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, ) Applications WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 79kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Eosinophil peroxidase heavy chain Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
EPX, eosinophil peroxidase, is an antimycobacterial protein that localizes to cytoplasmic granules of eosinophils and recruits bromide to generate a halogenating oxidant. EPX dependent generation of hypobromous acid causes damage to tissue during inflammatory conditions that include asthma, allergies,cancer and parasitic/helminthic infections. EPX is a major enzyme present in eosinophils and upon degranulation, becomes released into the airways of asthmatics. As a result of its cationic nature and its ability to catalyze the formation of highly toxic oxidants, EPX can induce lung injury in a JNK dependent manner.
Function:
Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis.
Subunit:
Tetramer of two light chains and two heavy chains.
Subcellular Location:
Cytoplasmic granule. Note=Cytoplasmic granules of eosinophils.
DISEASE:
Defects in EPX are the cause of eosinophil peroxidase deficiency (EPD) [MIM:261500]. EPD is an autosomal recessive defect where anomalous eosinophils are characterized by nuclear hypersegmentation, hypogranulation, and negative peroxidase and phospholipid staining.
Similarity:
Belongs to the peroxidase family. XPO subfamily.
Database links:
UniProtKB/Swiss-Prot: P11678.2
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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