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Product Name Anti-SMYD3/Biotin Chinese Name 生物素标记的组蛋白甲基转移酶SMYD3抗体 Alias SET and MYND domain containing 3; SET and MYND domain containing protein 3; SMYD 3; SMYD3 protein; Zinc finger MYND domain containing 1; Zinc finger MYND domain containing protein 1; Zinc finger protein subfamily 3A MYND domain containing 1; ZMYND 1; ZMYND1; ZNFN3A1; SMYD3_HUMAN. Research Area Tumour immunology Chromatin and nuclear signals transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Cow, Horse, Rabbit, Sheep, ) Applications WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 49kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SMYD3 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
SMYD3 is a gene that is over-expressed in the majority of colorectal carcinomas and hepatocellular carcinomas. SMYD3 forms a complex with RNA polymerase II through an interaction with the RNA helicase HELZ and transactivates a set of genes that included oncogenes, homeobox genes and genes associated with cell-cycle regulation. SMYD3 binds to a motif, 5'-CCCTCC-3', present in the promoter region of downstream genes such as Nkx2.8. The SET domain of SMYD3 shows histone H3-lysine 4 (H3-K4)-specific methyltransferase activity, which is enhanced in the presence of the heat-shock protein HSP90A.
Function:
Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences.
Subunit:
Interacts with HSPCA. Interacts with HELZ. Interacts with POLR2A; the interaction may be indirect and may be mediated by HELZ.
Subcellular Location:
Cytoplasm. Nucleus. Note=Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M.
Tissue Specificity:
Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.
Similarity:
Belongs to the histone-lysine methyltransferase family.
Contains 1 MYND-type zinc finger.
Contains 1 SET domain.
Database links:Entrez Gene: 64754 Human
Entrez Gene: 69726 Mouse
Omim: 608783 Human
SwissProt: Q9H7B4 Human
SwissProt: Q9CWR2 Mouse
Unigene: 567571 Human
Unigene: 222338 Mouse
Unigene: 28284 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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