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Rabbit Anti-MED30/AP Conjugated antibody
Rabbit Anti-MED30/AP Conjugated antibody
TRAP25;MED30; MED30_HUMAN; Mediator complex subunit 30; Mediator of RNA polymerase II transcription subunit 30; THRAP6; Thyroid hormone receptor-associated protein 6; Thyroid hormone receptor-associated protein complex 25 kDa component; TRAP/Mediator comp
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  • NO.:SL18769R-AP
    Clonality:Polyclonal
    Immunogen Species:Rabbit
    React Species:(predicted: Human,Mouse,Dog,Pig,Horse,Rabbit,Sheep,)
    Applications:WB IHC-P IHC-F ICC
    concentration:1mg/ml
  • Goods click count:50
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Product Name Anti-MED30/AP
Chinese Name 碱性磷酸酶(AP)标记的MED30蛋白抗体
Alias TRAP25; MED30; MED30_HUMAN; Mediator complex subunit 30; Mediator of RNA polymerase II transcription subunit 30; THRAP6; Thyroid hormone receptor-associated protein 6; Thyroid hormone receptor-associated protein complex 25 kDa component; TRAP/Mediator complex component TRAP25; Trap25.  
Research Area Cell biology  transcriptional regulatory factor  Epigenetics  
Immunogen Species Rabbit
Clonality Polyclonal
React Species (predicted: Human, Mouse, Dog, Pig, Horse, Rabbit, Sheep, )
Applications WB=1:50-200 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Molecular weight 20kDa
Form Lyophilized or Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthetic peptide derived from human MED30
Lsotype IgG
Purification affinity purified by Protein A
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Product Detail background:
The multiprotein TRAP/Mediator complex facilitates gene expression through a wide variety of transcriptional activators. MED30 is a component of this complex that appears to be metazoan specific (Baek et al., 2002 [PubMed 11909976]).[supplied by OMIM, Nov 2010]

Function:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Subcellular Location:
Nucleus.

Tissue Specificity:
Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.

Similarity:
Belongs to the Mediator complex subunit 30 family.

Database links:

Entrez Gene: 90390 Human

Entrez Gene: 69790 Mouse

Entrez Gene: 299905 Rat

Omim: 610237 Human

SwissProt: Q96HR3 Human

SwissProt: Q9CQI9 Mouse

Unigene: 492612 Human

Unigene: 195562 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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