TEL: +86 571 56623320 EMAIL: [email protected]
Product Name Anti-NEDL2/APC Chinese Name APC标记的E3Ubiquitin蛋白连接酶HECW2抗体 Alias C2 and WW domain-containing protein 2; E3 ubiquitin protein ligase HECW2; E3 ubiquitin-protein ligase HECW2; HECT; HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2; Hecw2; HECW2_HUMAN; KIAA130; KIAA1301; NEDD4 like E3 ubiquitin protein ligase 2; NEDD4 Related E3 Ubiquitin Ligase; NEDD4-like E3 ubiquitin-protein ligase 2. Research Area Cell biology Epigenetics Ubiquitin Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) Applications ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 176kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human NEDL2 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 癈 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20癈. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 癈. Product Detail background:
NEDL2 is a 1,572 amino acid cytoplasmic protein that is involved in protein modification and ubiquitination and exists as two alternatively spliced isoforms. NEDL2 acts as an E3 ubiquitin-protein ligase that enhances p73 transcription activation, ubiquitination and stabilization, and is highly expressed in adult heart, lung and brain. Containing one C2 domain, two WW domains and a single HECT (E6AP-type E3 ubiquitin-protein ligase) domain, NEDL2 is encoded by a gene that maps to human chromosome 2, which consists of 237 million bases, encodes over 1,400 genes and makes up approximately 8% of the human genome. A number of genetic diseases are linked to genes on chromosome 2 including Harlequin icthyosis, sitosterolemia and Alstr鰉 syndrome.
Function:
E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Predominantly expressed in adult brain, lung and heart.
Similarity:
Contains 1 C2 domain.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 2 WW domains.
Database links:Entrez Gene: 57520 Human
Entrez Gene: 329152 Mouse
SwissProt: Q9P2P5 Human
SwissProt: Q6I6G8 Mouse
Unigene: 654742 Human
Unigene: 132150 Mouse
Unigene: 447527 Mouse
Unigene: 198842 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Scan Wechat Qrcode