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Product Name Anti-IL18R Beta/AF647 Chinese Name AF647标记的白细胞介素-18受体β链抗体 Alias IL-18R Beta; Interleukin-18 receptor beta; Accessory protein like; IL18R Beta; AcPL; IL 18RacP; IL 1R accessor protein like; IL 1R7; IL 1RacPL; IL18Rbeta; Interleukin 18 receptor accessory protein; IL18RAP; Interleukin-18 receptor accessory protein-like; IL-18R-beta; IL-18Rbeta; Accessory protein-like; AcPL; Interleukin-1 receptor 7; IL-1R-7; IL-1R7; CD218 antigen-like family member B; CD218b; I18RA_HUMAN. JournalPMIDIFApplication[IF=5.51] Kobori et al. Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis. (2018) Front.Immunol. 9:334 FC ; Mouse.Research Area Cell biology immunology Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Dog, Horse, ) Applications IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 68kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human IL-18R beta Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
IL18R Beta is an accessory subunit of the heterodimeric receptor for IL18. This protein enhances the IL18 binding activity of IL18R1 (IL1RRP), a ligand binding subunit of IL18 receptor. The coexpression of IL18R1 and IL18R Beta is required for the activation of NF-kappaB and MAPK8 (JNK) in response to IL18.
Function:
Required for the high affinity binding of interleukin 18 (IL-18) to its receptor complex. Together with IL18R1 mediates IL-18-dependent activation of NF-kappa-B and JNK.
Subunit:
Together with IL18R1, probably belongs to the IL-18 receptor complex. Seems not to interact directly with IL-18.
Subcellular Location:
Membrane; Single-pass type I membrane protein.
Tissue Specificity:
Strongly expressed in peripheral blood leukocytes, spleen, lung, and, to a lesser extent, in colon, but not in any other tissue tested. Strongly expressed in T-cells polarized with IL-12. Specifically coexpressed with IL18R1 in Th1 cells.
Similarity:
Belongs to the interleukin-1 receptor family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain.
Database links:Entrez Gene: 8807 Human
Entrez Gene: 16174 Mouse
Omim: 604509 Human
SwissProt: O95256 Human
SwissProt: Q9Z2B1 Mouse
Unigene: 158315 Human
Unigene: 20466 Mouse
Unigene: 392521 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.Product Picture Blank control: K562.
Primary Antibody (green line): Rabbit Anti-IL18R Beta antibody (SL2616R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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